Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology
Phenolic compounds have shown to have a high bioactive potential against various pathologies, postulating as an interesting alternative to manage some diseases. In this sense, both <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> are two botanical sources with a demons...
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MDPI AG
2020-11-01
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| Seria: | Antioxidants |
| Hasła przedmiotowe: | |
| Dostęp online: | https://www.mdpi.com/2076-3921/9/12/1175 |
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| author | María del Carmen Villegas-Aguilar Francisco Javier Leyva-Jiménez María de la Luz Cádiz-Gurrea Antonio Segura-Carretero David Arráez-Román |
| author_facet | María del Carmen Villegas-Aguilar Francisco Javier Leyva-Jiménez María de la Luz Cádiz-Gurrea Antonio Segura-Carretero David Arráez-Román |
| author_sort | María del Carmen Villegas-Aguilar |
| collection | DOAJ |
| description | Phenolic compounds have shown to have a high bioactive potential against various pathologies, postulating as an interesting alternative to manage some diseases. In this sense, both <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> are two botanical sources with a demonstrated high bioactive potential, in which their antioxidant capacity stands out. In this work, the optimization of the extraction conditions for the recovery of phytochemicals from <i>L. citriodora</i> leaves and <i>H. sabdariffa</i> calyces has been carried out using Response Surface Methodologies (RSM) considering their total polar compounds measured by HPLC-ESI-TOF/MS and Folin-Ciocalteu assay, and its antioxidant capacity evaluated by Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays. The results showed that to maximize the antioxidant capacity in <i>H. sabdariffa</i>, a moderate temperature and high ethanol percentage are needed, while a low temperature and a high percentage of ethanol are needed in <i>L. citriodora</i>. In addition, with the results obtained in the multiple response analysis, it is possible to affirm the importance of this type of analysis to develop functional ingredients, taking into account both total content of phenolic compounds and their bioactivity. Furthermore, as confirmed in this study, these analyses can be extrapolated in different techniques and in different matrices, with phenolic compounds from different families being important to develop new high added value products for food, pharmaceutical or cosmetic industries. |
| first_indexed | 2024-03-10T14:34:58Z |
| format | Article |
| id | doaj.art-60304334e3a945d99ccfb587d62e522f |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T14:34:58Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-60304334e3a945d99ccfb587d62e522f2023-11-20T22:15:34ZengMDPI AGAntioxidants2076-39212020-11-01912117510.3390/antiox9121175Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface MethodologyMaría del Carmen Villegas-Aguilar0Francisco Javier Leyva-Jiménez1María de la Luz Cádiz-Gurrea2Antonio Segura-Carretero3David Arráez-Román4Department of Analytical Chemistry, University of Granada, 18071 Granada, SpainResearch and Development of Functional Food Centre (CIDAF), 18016 Granada, SpainDepartment of Analytical Chemistry, University of Granada, 18071 Granada, SpainDepartment of Analytical Chemistry, University of Granada, 18071 Granada, SpainDepartment of Analytical Chemistry, University of Granada, 18071 Granada, SpainPhenolic compounds have shown to have a high bioactive potential against various pathologies, postulating as an interesting alternative to manage some diseases. In this sense, both <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> are two botanical sources with a demonstrated high bioactive potential, in which their antioxidant capacity stands out. In this work, the optimization of the extraction conditions for the recovery of phytochemicals from <i>L. citriodora</i> leaves and <i>H. sabdariffa</i> calyces has been carried out using Response Surface Methodologies (RSM) considering their total polar compounds measured by HPLC-ESI-TOF/MS and Folin-Ciocalteu assay, and its antioxidant capacity evaluated by Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays. The results showed that to maximize the antioxidant capacity in <i>H. sabdariffa</i>, a moderate temperature and high ethanol percentage are needed, while a low temperature and a high percentage of ethanol are needed in <i>L. citriodora</i>. In addition, with the results obtained in the multiple response analysis, it is possible to affirm the importance of this type of analysis to develop functional ingredients, taking into account both total content of phenolic compounds and their bioactivity. Furthermore, as confirmed in this study, these analyses can be extrapolated in different techniques and in different matrices, with phenolic compounds from different families being important to develop new high added value products for food, pharmaceutical or cosmetic industries.https://www.mdpi.com/2076-3921/9/12/1175phenolic compoundsresponse surface methodology<i>Lippia citriodora</i><i>Hibiscus sabdariffa</i>antioxidantMicrowave-Assisted Extraction (MAE) |
| spellingShingle | María del Carmen Villegas-Aguilar Francisco Javier Leyva-Jiménez María de la Luz Cádiz-Gurrea Antonio Segura-Carretero David Arráez-Román Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology Antioxidants phenolic compounds response surface methodology <i>Lippia citriodora</i> <i>Hibiscus sabdariffa</i> antioxidant Microwave-Assisted Extraction (MAE) |
| title | Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology |
| title_full | Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology |
| title_fullStr | Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology |
| title_full_unstemmed | Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology |
| title_short | Comprehensive Analysis of Antioxidant Compounds from <i>Lippia citriodora</i> and <i>Hibiscus sabdariffa</i> Green Extracts Attained by Response Surface Methodology |
| title_sort | comprehensive analysis of antioxidant compounds from i lippia citriodora i and i hibiscus sabdariffa i green extracts attained by response surface methodology |
| topic | phenolic compounds response surface methodology <i>Lippia citriodora</i> <i>Hibiscus sabdariffa</i> antioxidant Microwave-Assisted Extraction (MAE) |
| url | https://www.mdpi.com/2076-3921/9/12/1175 |
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