BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum
Abstract The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrysanthemum plant remain unclear. In this study, the chrysanthemum BBX7 homolog CmBBX...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Molecular Horticulture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43897-023-00055-2 |
| _version_ | 1797853583647965184 |
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| author | Yiwen Zhai Yuqing Zhu Qi Wang Guohui Wang Yao Yu Lijun Wang Tao Liu Shenhui Liu Qian Hu Sumei Chen Fadi Chen Jiafu Jiang |
| author_facet | Yiwen Zhai Yuqing Zhu Qi Wang Guohui Wang Yao Yu Lijun Wang Tao Liu Shenhui Liu Qian Hu Sumei Chen Fadi Chen Jiafu Jiang |
| author_sort | Yiwen Zhai |
| collection | DOAJ |
| description | Abstract The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrysanthemum plant remain unclear. In this study, the chrysanthemum BBX7 homolog CmBBX7 was isolated and its flowering function was identified. The expression of CmBBX7 showed a diurnal rhythm and CmBBX7 exhibited higher expression levels than CmBBX8. Overexpression of CmBBX7 in transgenic chrysanthemum accelerated flowering, whereas lines transfected with a chimeric repressor (pSRDX-CmBBX7) exhibited delayed flowering. Yeast single hybridization, luciferase, electrophoretic mobility shift, and chromatin immunoprecipitation assays showed that CmBBX7 directly targets CmFTL1. In addition, we found that CmBBX7 and CmBBX8 interact to positively regulate the expression of CmFTL1 through binding to its promoter. Collectively, these results highlight CmBBX7 as a key cooperator in the BBX8–FT module to control chrysanthemum flowering. Graphical Abstract |
| first_indexed | 2024-04-09T19:51:53Z |
| format | Article |
| id | doaj.art-6030a1d969df491aa6e6949c58eab4a6 |
| institution | Directory Open Access Journal |
| issn | 2730-9401 |
| language | English |
| last_indexed | 2024-04-09T19:51:53Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Horticulture |
| spelling | doaj.art-6030a1d969df491aa6e6949c58eab4a62023-04-03T05:40:43ZengBMCMolecular Horticulture2730-94012023-04-013111310.1186/s43897-023-00055-2BBX7 interacts with BBX8 to accelerate flowering in chrysanthemumYiwen Zhai0Yuqing Zhu1Qi Wang2Guohui Wang3Yao Yu4Lijun Wang5Tao Liu6Shenhui Liu7Qian Hu8Sumei Chen9Fadi Chen10Jiafu Jiang11National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityNational Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural UniversityAbstract The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrysanthemum plant remain unclear. In this study, the chrysanthemum BBX7 homolog CmBBX7 was isolated and its flowering function was identified. The expression of CmBBX7 showed a diurnal rhythm and CmBBX7 exhibited higher expression levels than CmBBX8. Overexpression of CmBBX7 in transgenic chrysanthemum accelerated flowering, whereas lines transfected with a chimeric repressor (pSRDX-CmBBX7) exhibited delayed flowering. Yeast single hybridization, luciferase, electrophoretic mobility shift, and chromatin immunoprecipitation assays showed that CmBBX7 directly targets CmFTL1. In addition, we found that CmBBX7 and CmBBX8 interact to positively regulate the expression of CmFTL1 through binding to its promoter. Collectively, these results highlight CmBBX7 as a key cooperator in the BBX8–FT module to control chrysanthemum flowering. Graphical Abstracthttps://doi.org/10.1186/s43897-023-00055-2BBX familyDiurnal rhythmCmFTL1PhotoperiodFlowering transition |
| spellingShingle | Yiwen Zhai Yuqing Zhu Qi Wang Guohui Wang Yao Yu Lijun Wang Tao Liu Shenhui Liu Qian Hu Sumei Chen Fadi Chen Jiafu Jiang BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum Molecular Horticulture BBX family Diurnal rhythm CmFTL1 Photoperiod Flowering transition |
| title | BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum |
| title_full | BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum |
| title_fullStr | BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum |
| title_full_unstemmed | BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum |
| title_short | BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum |
| title_sort | bbx7 interacts with bbx8 to accelerate flowering in chrysanthemum |
| topic | BBX family Diurnal rhythm CmFTL1 Photoperiod Flowering transition |
| url | https://doi.org/10.1186/s43897-023-00055-2 |
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