The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran
Background & Aims: The inappropriate use of antibiotics has led to antibiotic resistance in microorganisms of Enterobacteriaceae family, especially in carbapenems. The aim of this study was to identify the carbapenemase producing Citrobacter frundii and Citrobacter koseri isolated from clinical...
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Kerman University of Medical Sciences
2015-12-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_16066_9b4f5b6d4ed9cfb9f8c91b9a971fa416.pdf |
| _version_ | 1827911845891014656 |
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| author | Alisha Akya Somayeh Jafari Kamal Ahmadi Azam Elahi |
| author_facet | Alisha Akya Somayeh Jafari Kamal Ahmadi Azam Elahi |
| author_sort | Alisha Akya |
| collection | DOAJ |
| description | Background & Aims: The inappropriate use of antibiotics has led to antibiotic resistance in microorganisms of Enterobacteriaceae family, especially in carbapenems. The aim of this study was to identify the carbapenemase producing Citrobacter frundii and Citrobacter koseri isolated from clinical specimens. Methods: One hundred Citrobacter isolates from various patient samples in Imam Reza Hospital, Kermanshah, Iran, were identified using the microbiologic differential tests and API-E20 Kit. After antibiotic susceptibility testing with disc, the isolates resisted to carbapenems were screened using MHT (Modified Hodge Test) for the presence of carbapenemases. Then, carbapenemase genes coded Verona integrinencoded metallo-beta-lactamase (VIM), Klebsiella pneumoniae carbapenemase (KPC), Imipenemase (IMP), New Delhi metallo-beta-lactamase-1 (NDM) were tested using specific primers via polymerase chain reaction (PCR) method. Results: From 100 isolates, 11 cases were carbapenem resistant. In the phenotypic screening test, 2 isolates were positive. PCR on isolates resistant to carbapenams confirmed VIM gene in 5 isolates: 3 (3.9%) of Citrobacter frundii and 2 (15.4%) of Citrobacter koseri. But the genes of blaKPC, blaVIM, blaIMP and blaNDM were not found in isolates. The highest and lowest antibiotic susceptibility were for meropenem (93%) and cefazolin (1%), respectively. Conclusion: It seems that the prevalence of carbapenemase genes in Citrobacter kosari and Citrobacter frundii was low in Kermanshah; however, VIM gene in these two species is probably more prevalent. This may suggest that most genes have not been high prevalent in this area yet. But, there may be other genes for resistance to carbapenems in our area which need further investigations. The results indicate that carbapenems are still effective antibiotics against Citrobacter species |
| first_indexed | 2024-03-13T02:08:08Z |
| format | Article |
| id | doaj.art-6031b662a9e64b1aa3336d00f22390d2 |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:08:08Z |
| publishDate | 2015-12-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-6031b662a9e64b1aa3336d00f22390d22023-07-01T05:24:52ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432015-12-0122662963816066The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, IranAlisha Akya0Somayeh Jafari1Kamal Ahmadi2Azam Elahi3Associate Professor, Department of Medical Microbiology, Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, IranMSc Student, Department of Microbiology, Imam Reaza Hospital, Kermanshah University of Medical Sciences, Kermanshah, IranMSc Student, Department of Medical Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranMSc Student, Department of Medical Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranBackground & Aims: The inappropriate use of antibiotics has led to antibiotic resistance in microorganisms of Enterobacteriaceae family, especially in carbapenems. The aim of this study was to identify the carbapenemase producing Citrobacter frundii and Citrobacter koseri isolated from clinical specimens. Methods: One hundred Citrobacter isolates from various patient samples in Imam Reza Hospital, Kermanshah, Iran, were identified using the microbiologic differential tests and API-E20 Kit. After antibiotic susceptibility testing with disc, the isolates resisted to carbapenems were screened using MHT (Modified Hodge Test) for the presence of carbapenemases. Then, carbapenemase genes coded Verona integrinencoded metallo-beta-lactamase (VIM), Klebsiella pneumoniae carbapenemase (KPC), Imipenemase (IMP), New Delhi metallo-beta-lactamase-1 (NDM) were tested using specific primers via polymerase chain reaction (PCR) method. Results: From 100 isolates, 11 cases were carbapenem resistant. In the phenotypic screening test, 2 isolates were positive. PCR on isolates resistant to carbapenams confirmed VIM gene in 5 isolates: 3 (3.9%) of Citrobacter frundii and 2 (15.4%) of Citrobacter koseri. But the genes of blaKPC, blaVIM, blaIMP and blaNDM were not found in isolates. The highest and lowest antibiotic susceptibility were for meropenem (93%) and cefazolin (1%), respectively. Conclusion: It seems that the prevalence of carbapenemase genes in Citrobacter kosari and Citrobacter frundii was low in Kermanshah; however, VIM gene in these two species is probably more prevalent. This may suggest that most genes have not been high prevalent in this area yet. But, there may be other genes for resistance to carbapenems in our area which need further investigations. The results indicate that carbapenems are still effective antibiotics against Citrobacter specieshttps://jkmu.kmu.ac.ir/article_16066_9b4f5b6d4ed9cfb9f8c91b9a971fa416.pdfcarbapenemasescitrobacter frundiicitrobacter koseri |
| spellingShingle | Alisha Akya Somayeh Jafari Kamal Ahmadi Azam Elahi The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran Journal of Kerman University of Medical Sciences carbapenemases citrobacter frundii citrobacter koseri |
| title | The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran |
| title_full | The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran |
| title_fullStr | The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran |
| title_full_unstemmed | The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran |
| title_short | The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran |
| title_sort | frequency of carbapenemase genes in citrobacter frundii and citrobacter koseri isolated from clinical specimens in imam reza hospital kermanshah iran |
| topic | carbapenemases citrobacter frundii citrobacter koseri |
| url | https://jkmu.kmu.ac.ir/article_16066_9b4f5b6d4ed9cfb9f8c91b9a971fa416.pdf |
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