Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins
Abstract Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-03-01
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| Series: | ChemistryOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/open.202200259 |
| _version_ | 1797859217099456512 |
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| author | Yulin Zhou Jicheng Niu Dr. Yan Zhou Prof. Dr. Fei Li |
| author_facet | Yulin Zhou Jicheng Niu Dr. Yan Zhou Prof. Dr. Fei Li |
| author_sort | Yulin Zhou |
| collection | DOAJ |
| description | Abstract Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO2 nanoparticles and encapsulated aqueous solution, we developed a LP‐based field‐amplified sample stacking (FASS) system for protein enrichment. The system was composed of a LP container, a sample solution and a Tris‐HCl solution containing hydroxyethyl cellulose (HEC). The system design, mechanism investigation, optimization of experimental parameters and characterization of LP‐FASS performance for protein enrichment were well studied. Under the optimized experimental conditions of 1 % HEC, 100 mm Tris‐HCl and 100 V in the LP‐FASS system, a 40–80 times enrichment of proteins was obtained in 40 min using bovine hemoglobin (BHb) as the model protein using the constructed LP‐FASS system. The simultaneous enrichment of multiple proteins (phycocyanin, BHb and cytochrome C) was also realized using the system. The LP‐FASS system can serve as a new platform for protein enrichment which is easy to be combined with online and offline detections. |
| first_indexed | 2024-04-09T21:25:53Z |
| format | Article |
| id | doaj.art-603468dc5e4c4ef5870b499fcc2ea418 |
| institution | Directory Open Access Journal |
| issn | 2191-1363 |
| language | English |
| last_indexed | 2024-04-09T21:25:53Z |
| publishDate | 2023-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj.art-603468dc5e4c4ef5870b499fcc2ea4182023-03-27T12:44:29ZengWiley-VCHChemistryOpen2191-13632023-03-01123n/an/a10.1002/open.202200259Liquid Plasticine‐Based Electrokinetic Enrichment of ProteinsYulin Zhou0Jicheng Niu1Dr. Yan Zhou2Prof. Dr. Fei Li3The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University 710049 Xi'an P. R. ChinaThe Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University 710049 Xi'an P. R. ChinaThe Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University 710049 Xi'an P. R. ChinaThe Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi'an Jiaotong University 710049 Xi'an P. R. ChinaAbstract Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO2 nanoparticles and encapsulated aqueous solution, we developed a LP‐based field‐amplified sample stacking (FASS) system for protein enrichment. The system was composed of a LP container, a sample solution and a Tris‐HCl solution containing hydroxyethyl cellulose (HEC). The system design, mechanism investigation, optimization of experimental parameters and characterization of LP‐FASS performance for protein enrichment were well studied. Under the optimized experimental conditions of 1 % HEC, 100 mm Tris‐HCl and 100 V in the LP‐FASS system, a 40–80 times enrichment of proteins was obtained in 40 min using bovine hemoglobin (BHb) as the model protein using the constructed LP‐FASS system. The simultaneous enrichment of multiple proteins (phycocyanin, BHb and cytochrome C) was also realized using the system. The LP‐FASS system can serve as a new platform for protein enrichment which is easy to be combined with online and offline detections.https://doi.org/10.1002/open.202200259electrophoresisfield amplification sample stackingliquid plasticineprotein enrichment |
| spellingShingle | Yulin Zhou Jicheng Niu Dr. Yan Zhou Prof. Dr. Fei Li Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins ChemistryOpen electrophoresis field amplification sample stacking liquid plasticine protein enrichment |
| title | Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins |
| title_full | Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins |
| title_fullStr | Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins |
| title_full_unstemmed | Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins |
| title_short | Liquid Plasticine‐Based Electrokinetic Enrichment of Proteins |
| title_sort | liquid plasticine based electrokinetic enrichment of proteins |
| topic | electrophoresis field amplification sample stacking liquid plasticine protein enrichment |
| url | https://doi.org/10.1002/open.202200259 |
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