Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares
After breeding or artificial insemination, especially with frozen/thawed semen, mares often develop a persistent uterine inflammation, which is diagnosed by intra-uterine fluid accumulation. Here, we explored whether intra-uterine fluid accumulation affects corpus luteum function and tested the hypo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/67 |
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| author | Francesca Freccero Beatrice Mislei Diego Bucci Francesco Dondi Gaetano Mari |
| author_facet | Francesca Freccero Beatrice Mislei Diego Bucci Francesco Dondi Gaetano Mari |
| author_sort | Francesca Freccero |
| collection | DOAJ |
| description | After breeding or artificial insemination, especially with frozen/thawed semen, mares often develop a persistent uterine inflammation, which is diagnosed by intra-uterine fluid accumulation. Here, we explored whether intra-uterine fluid accumulation affects corpus luteum function and tested the hypothesis that intra-uterine fluid accumulation after artificial insemination alters blood flow in the corpus luteum and plasma progesterone concentrations. A total of 40 Standardbred mares were artificially inseminated with frozen-thawed semen 30 to 36 h after induction of ovulation, and cases with or without intra-uterine fluid accumulation were detected by ultrasound 12 h after insemination. Luteal blood flow was measured by Power Doppler ultrasonography 3 and 6 days after ovulation, progesterone concentration was measured in peripheral plasma by ELISA 6 days after ovulation, and pregnancy was diagnosed by ultrasonography 14 days after ovulation. Luteal blood flow increased between 3 and 6 days after ovulation, but blood flow did not differ significantly between cases with (n = 28) and without (n = 25) intra-uterine fluid accumulation after insemination. Surprisingly, progesterone concentrations were higher in cases of intra-uterine fluid accumulation than cases without (9.3 ± 1.1 vs. 6.6 ± 0.5 ng/mL, <i>p</i> = 0.048). Pregnancy was less likely in cases with intra-uterine fluid accumulation than in cases without (10/28 vs. 17/25, <i>p</i> = 0.019), and there was a negative correlation between the severity of intra-uterine fluid accumulation and per cycle pregnancy rate. These data suggest that although intra-uterine fluid accumulation increases the secretion of progesterone, pregnancy is more dependent on uterine health than ovarian function. |
| first_indexed | 2024-03-11T10:12:46Z |
| format | Article |
| id | doaj.art-6034aba51bce4f969094ed3131a25e0d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:12:46Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6034aba51bce4f969094ed3131a25e0d2023-11-16T14:18:40ZengMDPI AGAnimals2076-26152022-12-011316710.3390/ani13010067Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in MaresFrancesca Freccero0Beatrice Mislei1Diego Bucci2Francesco Dondi3Gaetano Mari4DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyAUB INFA National Institute of Artificial Insemination, University of Bologna, Via Gandolfi 16, 40057 Cadriano, ItalyDIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyDIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyDIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyAfter breeding or artificial insemination, especially with frozen/thawed semen, mares often develop a persistent uterine inflammation, which is diagnosed by intra-uterine fluid accumulation. Here, we explored whether intra-uterine fluid accumulation affects corpus luteum function and tested the hypothesis that intra-uterine fluid accumulation after artificial insemination alters blood flow in the corpus luteum and plasma progesterone concentrations. A total of 40 Standardbred mares were artificially inseminated with frozen-thawed semen 30 to 36 h after induction of ovulation, and cases with or without intra-uterine fluid accumulation were detected by ultrasound 12 h after insemination. Luteal blood flow was measured by Power Doppler ultrasonography 3 and 6 days after ovulation, progesterone concentration was measured in peripheral plasma by ELISA 6 days after ovulation, and pregnancy was diagnosed by ultrasonography 14 days after ovulation. Luteal blood flow increased between 3 and 6 days after ovulation, but blood flow did not differ significantly between cases with (n = 28) and without (n = 25) intra-uterine fluid accumulation after insemination. Surprisingly, progesterone concentrations were higher in cases of intra-uterine fluid accumulation than cases without (9.3 ± 1.1 vs. 6.6 ± 0.5 ng/mL, <i>p</i> = 0.048). Pregnancy was less likely in cases with intra-uterine fluid accumulation than in cases without (10/28 vs. 17/25, <i>p</i> = 0.019), and there was a negative correlation between the severity of intra-uterine fluid accumulation and per cycle pregnancy rate. These data suggest that although intra-uterine fluid accumulation increases the secretion of progesterone, pregnancy is more dependent on uterine health than ovarian function.https://www.mdpi.com/2076-2615/13/1/67corpus luteumblood flowprogesteroneintra-uterine fluid accumulationmares |
| spellingShingle | Francesca Freccero Beatrice Mislei Diego Bucci Francesco Dondi Gaetano Mari Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares Animals corpus luteum blood flow progesterone intra-uterine fluid accumulation mares |
| title | Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares |
| title_full | Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares |
| title_fullStr | Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares |
| title_full_unstemmed | Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares |
| title_short | Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares |
| title_sort | effects of intra uterine fluid accumulation after artificial insemination on luteal function in mares |
| topic | corpus luteum blood flow progesterone intra-uterine fluid accumulation mares |
| url | https://www.mdpi.com/2076-2615/13/1/67 |
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