Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii
Abstract Background The hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. Howe...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-6042-1 |
| _version_ | 1818258023725924352 |
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| author | Chaima Bensaoud Hajer Aounallah Juliana Mozer Sciani Fernanda Faria Ana Marisa Chudzinski-Tavassi Ali Bouattour Youmna M’ghirbi |
| author_facet | Chaima Bensaoud Hajer Aounallah Juliana Mozer Sciani Fernanda Faria Ana Marisa Chudzinski-Tavassi Ali Bouattour Youmna M’ghirbi |
| author_sort | Chaima Bensaoud |
| collection | DOAJ |
| description | Abstract Background The hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. Results We reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. Conclusion This investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully. |
| first_indexed | 2024-12-12T17:52:57Z |
| format | Article |
| id | doaj.art-60362dc7bd274cf2bcb01daa356d240a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T17:52:57Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-60362dc7bd274cf2bcb01daa356d240a2022-12-22T00:16:47ZengBMCBMC Genomics1471-21642019-08-0120111210.1186/s12864-019-6042-1Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedariiChaima Bensaoud0Hajer Aounallah1Juliana Mozer Sciani2Fernanda Faria3Ana Marisa Chudzinski-Tavassi4Ali Bouattour5Youmna M’ghirbi6Université de Tunis El Manar, Institut Pasteur de Tunis, LR11IPT03, Service d’entomologie médicaleUniversité de Tunis El Manar, Institut Pasteur de Tunis, LR11IPT03, Service d’entomologie médicaleLaboratório de Biologia Molecular, Instituto ButantanLaboratório de Biologia Molecular, Instituto ButantanLaboratório de Biologia Molecular, Instituto ButantanUniversité de Tunis El Manar, Institut Pasteur de Tunis, LR11IPT03, Service d’entomologie médicaleUniversité de Tunis El Manar, Institut Pasteur de Tunis, LR11IPT03, Service d’entomologie médicaleAbstract Background The hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. Results We reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. Conclusion This investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully.http://link.springer.com/article/10.1186/s12864-019-6042-1Hyalomma dromedariiSalivary glandsProteomeLC–MS/MSPIT |
| spellingShingle | Chaima Bensaoud Hajer Aounallah Juliana Mozer Sciani Fernanda Faria Ana Marisa Chudzinski-Tavassi Ali Bouattour Youmna M’ghirbi Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii BMC Genomics Hyalomma dromedarii Salivary glands Proteome LC–MS/MS PIT |
| title | Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii |
| title_full | Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii |
| title_fullStr | Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii |
| title_full_unstemmed | Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii |
| title_short | Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii |
| title_sort | proteomic informed by transcriptomic for salivary glands components of the camel tick hyalomma dromedarii |
| topic | Hyalomma dromedarii Salivary glands Proteome LC–MS/MS PIT |
| url | http://link.springer.com/article/10.1186/s12864-019-6042-1 |
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