Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA
<i>Lobelia chinensis</i> Lour. (<i>L. chinensis</i>) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. <i>L. chinensis<...
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MDPI AG
2021-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/24/12080 |
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| author | Beom-Geun Jo Young-Hun Park Ki Hyun Kim Su-Nam Kim Min Hye Yang |
| author_facet | Beom-Geun Jo Young-Hun Park Ki Hyun Kim Su-Nam Kim Min Hye Yang |
| author_sort | Beom-Geun Jo |
| collection | DOAJ |
| description | <i>Lobelia chinensis</i> Lour. (<i>L. chinensis</i>) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. <i>L. chinensis</i> contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in <i>L. chinensis</i> for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-glucuronopyranoside, clerodendrin, chrysoeriol-7-<i>O</i>-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (<i>r</i><sup>2</sup> > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in <i>L. chinensis</i>. |
| first_indexed | 2024-03-10T04:36:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:36:36Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-60364b36d9a3431ead31917f65a5cccc2023-11-23T03:42:55ZengMDPI AGApplied Sciences2076-34172021-12-0111241208010.3390/app112412080Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDABeom-Geun Jo0Young-Hun Park1Ki Hyun Kim2Su-Nam Kim3Min Hye Yang4Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, KoreaNatural Product Research Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaNatural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, KoreaDepartment of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea<i>Lobelia chinensis</i> Lour. (<i>L. chinensis</i>) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. <i>L. chinensis</i> contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in <i>L. chinensis</i> for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-glucuronopyranoside, clerodendrin, chrysoeriol-7-<i>O</i>-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (<i>r</i><sup>2</sup> > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in <i>L. chinensis</i>.https://www.mdpi.com/2076-3417/11/24/12080<i>Lobelia chinensis</i> Lour.luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranosideclerodendrinchrysoeriol-7-<i>O</i>-diglucuronidediosminHPLC-PDA |
| spellingShingle | Beom-Geun Jo Young-Hun Park Ki Hyun Kim Su-Nam Kim Min Hye Yang Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA Applied Sciences <i>Lobelia chinensis</i> Lour. luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranoside clerodendrin chrysoeriol-7-<i>O</i>-diglucuronide diosmin HPLC-PDA |
| title | Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA |
| title_full | Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA |
| title_fullStr | Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA |
| title_full_unstemmed | Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA |
| title_short | Simultaneous Determination of Four Marker Compounds in <i>Lobelia chinensis</i> Lour. Extract by HPLC-PDA |
| title_sort | simultaneous determination of four marker compounds in i lobelia chinensis i lour extract by hplc pda |
| topic | <i>Lobelia chinensis</i> Lour. luteolin-7-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranosyl (1→2)-<i>O</i>-β-<span style="font-variant: small-caps">d</span>-glucuronopyranoside clerodendrin chrysoeriol-7-<i>O</i>-diglucuronide diosmin HPLC-PDA |
| url | https://www.mdpi.com/2076-3417/11/24/12080 |
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