Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane
Endotoxemia is thought to induce severe changes in coagulation status. In this study, blood samples from six beagle dogs receiving 1 mg/kg <i>E. coli</i> lipopolysaccharide (LPS) intravenously were analyzed to describe the concurrent changes in platelet count, platelet function assessed...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/19/2997 |
| _version_ | 1797576317689921536 |
|---|---|
| author | Annette P. N. Kutter Fabiola B. Joerger Barbara Riond Barbara Steblaj |
| author_facet | Annette P. N. Kutter Fabiola B. Joerger Barbara Riond Barbara Steblaj |
| author_sort | Annette P. N. Kutter |
| collection | DOAJ |
| description | Endotoxemia is thought to induce severe changes in coagulation status. In this study, blood samples from six beagle dogs receiving 1 mg/kg <i>E. coli</i> lipopolysaccharide (LPS) intravenously were analyzed to describe the concurrent changes in platelet count, platelet function assessed with impedance thromboaggregometry, thromboelastometry and d-dimers during artificially induced endotoxemia and its therapy with fluids and vasopressors at five timepoints (baseline, after LPS and 30 mL/kg Ringer’s acetate, during noradrenaline ± dexmedetomidine infusion, after a second fluid bolus and a second time after vasopressors). Results were analyzed for changes over time with the Friedman test, and statistical significance was set at <i>p</i> < 0.05. We found decreased platelet count and function and changes in all platelet-associated rotational thromboelastometry (ROTEM) variables indicating hypocoagulability, as well as increases in d-dimers indicating fibrinolysis within one hour of intravenous administration of LPS, with partial recovery of values after treatment and over time. The fast changes in platelet count, platelet function and ROTEM variables reflect the large impact of endotoxemia on the coagulation system and support repeated evaluation during the progress of endotoxemic diseases. The partial recovery of the variables after initiation of fluid and vasopressor therapy may reflect the positive impact of the currently suggested therapeutic interventions during septic shock in dogs. |
| first_indexed | 2024-03-10T21:50:32Z |
| format | Article |
| id | doaj.art-6037359c1d8f46a7ad1d2a0fbcf95c22 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T21:50:32Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6037359c1d8f46a7ad1d2a0fbcf95c222023-11-19T13:58:48ZengMDPI AGAnimals2076-26152023-09-011319299710.3390/ani13192997Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with SevofluraneAnnette P. N. Kutter0Fabiola B. Joerger1Barbara Riond2Barbara Steblaj3Section of Anaesthesiology, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandSection of Anaesthesiology, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandClinical Laboratory, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandSection of Anaesthesiology, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandEndotoxemia is thought to induce severe changes in coagulation status. In this study, blood samples from six beagle dogs receiving 1 mg/kg <i>E. coli</i> lipopolysaccharide (LPS) intravenously were analyzed to describe the concurrent changes in platelet count, platelet function assessed with impedance thromboaggregometry, thromboelastometry and d-dimers during artificially induced endotoxemia and its therapy with fluids and vasopressors at five timepoints (baseline, after LPS and 30 mL/kg Ringer’s acetate, during noradrenaline ± dexmedetomidine infusion, after a second fluid bolus and a second time after vasopressors). Results were analyzed for changes over time with the Friedman test, and statistical significance was set at <i>p</i> < 0.05. We found decreased platelet count and function and changes in all platelet-associated rotational thromboelastometry (ROTEM) variables indicating hypocoagulability, as well as increases in d-dimers indicating fibrinolysis within one hour of intravenous administration of LPS, with partial recovery of values after treatment and over time. The fast changes in platelet count, platelet function and ROTEM variables reflect the large impact of endotoxemia on the coagulation system and support repeated evaluation during the progress of endotoxemic diseases. The partial recovery of the variables after initiation of fluid and vasopressor therapy may reflect the positive impact of the currently suggested therapeutic interventions during septic shock in dogs.https://www.mdpi.com/2076-2615/13/19/2997canined-dimershemostasisplateletssepsisthromboaggregometry |
| spellingShingle | Annette P. N. Kutter Fabiola B. Joerger Barbara Riond Barbara Steblaj Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane Animals canine d-dimers hemostasis platelets sepsis thromboaggregometry |
| title | Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane |
| title_full | Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane |
| title_fullStr | Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane |
| title_full_unstemmed | Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane |
| title_short | Evaluation of the Effect of Induced Endotoxemia on ROTEM S<sup>®</sup> and Platelet Parameters in Beagle Dogs Anaesthetized with Sevoflurane |
| title_sort | evaluation of the effect of induced endotoxemia on rotem s sup r sup and platelet parameters in beagle dogs anaesthetized with sevoflurane |
| topic | canine d-dimers hemostasis platelets sepsis thromboaggregometry |
| url | https://www.mdpi.com/2076-2615/13/19/2997 |
| work_keys_str_mv | AT annettepnkutter evaluationoftheeffectofinducedendotoxemiaonrotemssupsupandplateletparametersinbeagledogsanaesthetizedwithsevoflurane AT fabiolabjoerger evaluationoftheeffectofinducedendotoxemiaonrotemssupsupandplateletparametersinbeagledogsanaesthetizedwithsevoflurane AT barbarariond evaluationoftheeffectofinducedendotoxemiaonrotemssupsupandplateletparametersinbeagledogsanaesthetizedwithsevoflurane AT barbarasteblaj evaluationoftheeffectofinducedendotoxemiaonrotemssupsupandplateletparametersinbeagledogsanaesthetizedwithsevoflurane |