ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L)
Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive...
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Universitas Syiah Kuala, Faculty of Mathematics and Natural Science
2019-06-01
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| Series: | Jurnal Natural |
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| Online Access: | http://jurnal.unsyiah.ac.id/natural/article/view/12568 |
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| author | Binawati Ginting Ilham Maulana Nurdin Saidi Syarifah Yanti Astryna |
| author_facet | Binawati Ginting Ilham Maulana Nurdin Saidi Syarifah Yanti Astryna |
| author_sort | Binawati Ginting |
| collection | DOAJ |
| description | Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class. |
| first_indexed | 2024-04-24T14:41:38Z |
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| id | doaj.art-603ad3862e084b58a1a2f363ca6879d4 |
| institution | Directory Open Access Journal |
| issn | 1411-8513 2541-4062 |
| language | English |
| last_indexed | 2024-04-24T14:41:38Z |
| publishDate | 2019-06-01 |
| publisher | Universitas Syiah Kuala, Faculty of Mathematics and Natural Science |
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| series | Jurnal Natural |
| spelling | doaj.art-603ad3862e084b58a1a2f363ca6879d42024-04-02T21:43:24ZengUniversitas Syiah Kuala, Faculty of Mathematics and Natural ScienceJurnal Natural1411-85132541-40622019-06-01192495310.24815/jn.v19i2.125689968ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L)Binawati Ginting0Ilham Maulana1Nurdin Saidi2Syarifah Yanti Astryna3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Banda Aceh, 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Banda Aceh, 23111, IndonesiaIsolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class.http://jurnal.unsyiah.ac.id/natural/article/view/12568Cacao pod husk (Theobroma cacao L), Ethyl acetate extract, Antioxidant and 1,1-diphenyl-2-Pycrilhidrazil (DPPH). |
| spellingShingle | Binawati Ginting Ilham Maulana Nurdin Saidi Syarifah Yanti Astryna ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) Jurnal Natural Cacao pod husk (Theobroma cacao L), Ethyl acetate extract, Antioxidant and 1,1-diphenyl-2-Pycrilhidrazil (DPPH). |
| title | ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) |
| title_full | ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) |
| title_fullStr | ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) |
| title_full_unstemmed | ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) |
| title_short | ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) |
| title_sort | isolation and activity antioxidant test of cocoa pod husk ethyl asetat extracts theobroma cacao l |
| topic | Cacao pod husk (Theobroma cacao L), Ethyl acetate extract, Antioxidant and 1,1-diphenyl-2-Pycrilhidrazil (DPPH). |
| url | http://jurnal.unsyiah.ac.id/natural/article/view/12568 |
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