Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-05-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00251/full |
| _version_ | 1819227206142918656 |
|---|---|
| author | Marco Kremp Elena Bittner Domenica Martorana Alexander Klingenberger Thorsten Stehlik Michael Bölker Michael Bölker Johannes Freitag |
| author_facet | Marco Kremp Elena Bittner Domenica Martorana Alexander Klingenberger Thorsten Stehlik Michael Bölker Michael Bölker Johannes Freitag |
| author_sort | Marco Kremp |
| collection | DOAJ |
| description | Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type 2 peroxisomal targeting signal encoded in the 5′ untranslated region of fungal genes coding for 6-phosphogluconate dehydrogenase (PGD), a key enzyme of the oxidative pentose phosphate pathway. The conservation of the cryptic PTS2 motif suggests a biological function. We observed that translation from a non-AUG start codon generates an N-terminally extended peroxisomal isoform of Ustilago maydis PGD. Non-canonical initiation occurred at the sequence AGG AUU, consisting of two near-cognate start codons in tandem. Taken together, our data reveal non-AUG translation initiation as an additional mechanism to achieve the dual localization of a protein required both in the cytosol and the peroxisomes. |
| first_indexed | 2024-12-23T10:37:42Z |
| format | Article |
| id | doaj.art-6042441c79a843a5a1b9312408bec361 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-23T10:37:42Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-6042441c79a843a5a1b9312408bec3612022-12-21T17:50:15ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-05-01810.3389/fcell.2020.00251515949Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in FungiMarco Kremp0Elena Bittner1Domenica Martorana2Alexander Klingenberger3Thorsten Stehlik4Michael Bölker5Michael Bölker6Johannes Freitag7Department of Biology, Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyLOEWE Center for Synthetic Microbiology, Marburg, GermanyDepartment of Biology, Philipps-University Marburg, Marburg, GermanyProteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type 2 peroxisomal targeting signal encoded in the 5′ untranslated region of fungal genes coding for 6-phosphogluconate dehydrogenase (PGD), a key enzyme of the oxidative pentose phosphate pathway. The conservation of the cryptic PTS2 motif suggests a biological function. We observed that translation from a non-AUG start codon generates an N-terminally extended peroxisomal isoform of Ustilago maydis PGD. Non-canonical initiation occurred at the sequence AGG AUU, consisting of two near-cognate start codons in tandem. Taken together, our data reveal non-AUG translation initiation as an additional mechanism to achieve the dual localization of a protein required both in the cytosol and the peroxisomes.https://www.frontiersin.org/article/10.3389/fcell.2020.00251/fulldual targetingpentose-phosphate pathwaynon-AUG translationredox-shuttleperoxisome |
| spellingShingle | Marco Kremp Elena Bittner Domenica Martorana Alexander Klingenberger Thorsten Stehlik Michael Bölker Michael Bölker Johannes Freitag Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi Frontiers in Cell and Developmental Biology dual targeting pentose-phosphate pathway non-AUG translation redox-shuttle peroxisome |
| title | Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi |
| title_full | Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi |
| title_fullStr | Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi |
| title_full_unstemmed | Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi |
| title_short | Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi |
| title_sort | non aug translation initiation generates peroxisomal isoforms of 6 phosphogluconate dehydrogenase in fungi |
| topic | dual targeting pentose-phosphate pathway non-AUG translation redox-shuttle peroxisome |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00251/full |
| work_keys_str_mv | AT marcokremp nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT elenabittner nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT domenicamartorana nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT alexanderklingenberger nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT thorstenstehlik nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT michaelbolker nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT michaelbolker nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi AT johannesfreitag nonaugtranslationinitiationgeneratesperoxisomalisoformsof6phosphogluconatedehydrogenaseinfungi |