<i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production
In vitro shoot culture of the endangered medicinal plant <i>Gentianella lutescens</i> was established from epicotyl explants cultured on MS basal medium with 0.2 mg L<sup>−1</sup> 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot line...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1651 |
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| author | Dijana Krstić-Milošević Nevena Banjac Teodora Janković Dragan Vinterhalter Branka Vinterhalter |
| author_facet | Dijana Krstić-Milošević Nevena Banjac Teodora Janković Dragan Vinterhalter Branka Vinterhalter |
| author_sort | Dijana Krstić-Milošević |
| collection | DOAJ |
| description | In vitro shoot culture of the endangered medicinal plant <i>Gentianella lutescens</i> was established from epicotyl explants cultured on MS basal medium with 0.2 mg L<sup>−1</sup> 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L<sup>−1</sup> indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-<i>O</i>-glucoside and bellidifolin-8-<i>O</i>-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-<i>O</i>-glucoside, and bellidifolin-8-<i>O</i>-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of <i>G. lutescens</i> and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T08:27:18Z |
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| spelling | doaj.art-6042a3471a0f4d0b89b2aa1e4b6d46762023-11-22T09:19:35ZengMDPI AGPlants2223-77472021-08-01108165110.3390/plants10081651<i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones ProductionDijana Krstić-Milošević0Nevena Banjac1Teodora Janković2Dragan Vinterhalter3Branka Vinterhalter4Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, SerbiaInsitute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, SerbiaIn vitro shoot culture of the endangered medicinal plant <i>Gentianella lutescens</i> was established from epicotyl explants cultured on MS basal medium with 0.2 mg L<sup>−1</sup> 6-benzylaminopurine (BA) and evaluated for xanthones content for the first time. Five shoot lines were obtained and no significant variations in multiplication rate, shoot elongation, and xanthones profile were found among them. The highest rooting rate (33.3%) was achieved by shoots treated for 2 days with 5 mg L<sup>−1</sup> indole-3-butyric acid (IBA) followed by cultivation in liquid PGR-free ½ MS medium for 60 days. HPLC analysis revealed the lower content of xanthones—mangiferin, bellidifolin, demethylbellidifolin, demethylbellidifolin-8-<i>O</i>-glucoside and bellidifolin-8-<i>O</i>-glucoside—in in vitro cultured shoots compared to wild growing plants. The increasing concentration of sucrose, sorbitol and abiotic elicitors salicylic acid (SA), jasmonic acid (JA) and methyl jasmonate (MeJA) altered shoot growth and xanthone production. Sucrose and sorbitol applied at the highest concentration of 233.6 mM increased dry matter percentage, while SA at 100 μM promoted shoot growth 2-fold. The increased sucrose concentration enhanced accumulation of xanthones in shoot cultures 2–3-fold compared to the control shoots. Elicitors at 100–300 μM increased the accumulation of mangiferin, demethylbellidifolin-8-<i>O</i>-glucoside, and bellidifolin-8-<i>O</i>-glucoside almost equally, while MeJA at the highest concentration of 500 μM enhanced amount of aglycones demethylbellidifolin and bellidifolin 7-fold compared to the control. The obtained results facilitate conservation of <i>G. lutescens</i> and pave the way for further research on large-scale shoot propagation and production of pharmacologically active xanthones.https://www.mdpi.com/2223-7747/10/8/1651shoot culturesecondary metabolitesHPLCbellidifolinosmotic stress |
| spellingShingle | Dijana Krstić-Milošević Nevena Banjac Teodora Janković Dragan Vinterhalter Branka Vinterhalter <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production Plants shoot culture secondary metabolites HPLC bellidifolin osmotic stress |
| title | <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production |
| title_full | <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production |
| title_fullStr | <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production |
| title_full_unstemmed | <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production |
| title_short | <i>Gentianella lutescens</i> subsp. <i>carpatica</i> J. Holub.: Shoot Propagation In Vitro and Effect of Sucrose and Elicitors on Xanthones Production |
| title_sort | i gentianella lutescens i subsp i carpatica i j holub shoot propagation in vitro and effect of sucrose and elicitors on xanthones production |
| topic | shoot culture secondary metabolites HPLC bellidifolin osmotic stress |
| url | https://www.mdpi.com/2223-7747/10/8/1651 |
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