LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA
Abstract Background Bladder cancer is a prevalent malignancy of the urinary system, in which long non-coding RNAs (lncRNAs) are highly associated. We aimed to elucidate the role of LINC00958 in bladder cancer. Methods LINC00958 expression levels were measured using qRT-PCR. The interaction of LINC00...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-10-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-021-08882-6 |
| _version_ | 1818896944912662528 |
|---|---|
| author | Hongtao Zhen Peng Du Qiang Yi Xiaolong Tang Tongqing Wang |
| author_facet | Hongtao Zhen Peng Du Qiang Yi Xiaolong Tang Tongqing Wang |
| author_sort | Hongtao Zhen |
| collection | DOAJ |
| description | Abstract Background Bladder cancer is a prevalent malignancy of the urinary system, in which long non-coding RNAs (lncRNAs) are highly associated. We aimed to elucidate the role of LINC00958 in bladder cancer. Methods LINC00958 expression levels were measured using qRT-PCR. The interaction of LINC00958-miR-490-3p-AURKA was analyzed by luciferase, RIP, and RNA pull-down assays. The biological roles of LINC00958, miR-490-3p, and AURKA in bladder cancer cells were analyzed using CCK8, BrdU, and transwell assays. Results Increased expression of LINC00958 and AURKA was observed in bladder cancer tissues and cell lines. Decreased LINC00958 expression repressed bladder cancer progression and downregulation of miR-490-3p accelerated bladder cancer cell progression. Moreover, LINC00958 sponges miR-490-3p to upregulate AURKA expression, thereby promoting carcinogenesis in bladder cancer cells. Conclusions Our study revealed that LINC00958 facilitated cell proliferation and invasion, and suppressed cell apoptosis by sponging miR-490-3p and upregulating AURKA, thus inspiring a new treatment method for bladder cancer. |
| first_indexed | 2024-12-19T19:08:20Z |
| format | Article |
| id | doaj.art-604426a93d6f44a0932a0b273e9abc81 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-19T19:08:20Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-604426a93d6f44a0932a0b273e9abc812022-12-21T20:09:23ZengBMCBMC Cancer1471-24072021-10-0121111410.1186/s12885-021-08882-6LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKAHongtao Zhen0Peng Du1Qiang Yi2Xiaolong Tang3Tongqing Wang4Department of Urology Ward 1, Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityDepartment of Urology, Peking University Cancer HospitalDepartment of Urology Ward 1, Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityDepartment of Urology Ward 1, Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityDepartment of Urology Ward 1, Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityAbstract Background Bladder cancer is a prevalent malignancy of the urinary system, in which long non-coding RNAs (lncRNAs) are highly associated. We aimed to elucidate the role of LINC00958 in bladder cancer. Methods LINC00958 expression levels were measured using qRT-PCR. The interaction of LINC00958-miR-490-3p-AURKA was analyzed by luciferase, RIP, and RNA pull-down assays. The biological roles of LINC00958, miR-490-3p, and AURKA in bladder cancer cells were analyzed using CCK8, BrdU, and transwell assays. Results Increased expression of LINC00958 and AURKA was observed in bladder cancer tissues and cell lines. Decreased LINC00958 expression repressed bladder cancer progression and downregulation of miR-490-3p accelerated bladder cancer cell progression. Moreover, LINC00958 sponges miR-490-3p to upregulate AURKA expression, thereby promoting carcinogenesis in bladder cancer cells. Conclusions Our study revealed that LINC00958 facilitated cell proliferation and invasion, and suppressed cell apoptosis by sponging miR-490-3p and upregulating AURKA, thus inspiring a new treatment method for bladder cancer.https://doi.org/10.1186/s12885-021-08882-6LINC00958miR-490-3pAURKABladder cancer |
| spellingShingle | Hongtao Zhen Peng Du Qiang Yi Xiaolong Tang Tongqing Wang LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA BMC Cancer LINC00958 miR-490-3p AURKA Bladder cancer |
| title | LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA |
| title_full | LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA |
| title_fullStr | LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA |
| title_full_unstemmed | LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA |
| title_short | LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA |
| title_sort | linc00958 promotes bladder cancer carcinogenesis by targeting mir 490 3p and aurka |
| topic | LINC00958 miR-490-3p AURKA Bladder cancer |
| url | https://doi.org/10.1186/s12885-021-08882-6 |
| work_keys_str_mv | AT hongtaozhen linc00958promotesbladdercancercarcinogenesisbytargetingmir4903pandaurka AT pengdu linc00958promotesbladdercancercarcinogenesisbytargetingmir4903pandaurka AT qiangyi linc00958promotesbladdercancercarcinogenesisbytargetingmir4903pandaurka AT xiaolongtang linc00958promotesbladdercancercarcinogenesisbytargetingmir4903pandaurka AT tongqingwang linc00958promotesbladdercancercarcinogenesisbytargetingmir4903pandaurka |