A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates
Cerebrospinal fluid (CSF) is an important sample source for diagnosing diseases in the central nervous system (CNS), but collecting and injecting CSF in small animals is technically challenging and often results in high mortality rates. Here, we present a cost-effective and efficient method for acce...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1609 |
| _version_ | 1797595958476800000 |
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| author | Jingrong Regina Han Yu Yang Tianshu William Wu Tao-Tao Shi Wenlu Li Yilong Zou |
| author_facet | Jingrong Regina Han Yu Yang Tianshu William Wu Tao-Tao Shi Wenlu Li Yilong Zou |
| author_sort | Jingrong Regina Han |
| collection | DOAJ |
| description | Cerebrospinal fluid (CSF) is an important sample source for diagnosing diseases in the central nervous system (CNS), but collecting and injecting CSF in small animals is technically challenging and often results in high mortality rates. Here, we present a cost-effective and efficient method for accessing the CSF in live rodents for fluid collection and infusion purposes. The key element of this protocol is a metal needle tool bent at a unique angle and length, allowing the successful access of the CSF through the foramen magnum. With this method, we can collect 5–10 µL of the CSF from mice and 70–100 µL from rats for downstream analyses, including mass spectrometry. Moreover, our minimally-invasive procedure enables iterative CSF collection from the same animal every few days, representing a significant improvement over prior protocols. Additionally, our method can be used to inject solutions into mice cisterna magna with high success rates and high postoperative recovery rates. In summary, we provide an efficient and minimally-invasive protocol for collecting and infusing reagents into the CSF in live rodents. We envision this protocol will facilitate biomarker discovery and drug development for diseases in the central nervous system. |
| first_indexed | 2024-03-11T02:44:43Z |
| format | Article |
| id | doaj.art-60458891f2b5431f8d0a130bf9dca699 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:44:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-60458891f2b5431f8d0a130bf9dca6992023-11-18T09:25:43ZengMDPI AGBiomedicines2227-90592023-06-01116160910.3390/biomedicines11061609A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival RatesJingrong Regina Han0Yu Yang1Tianshu William Wu2Tao-Tao Shi3Wenlu Li4Yilong Zou5School of Life Sciences, Fudan University, Shanghai 200438, ChinaLaboratory Animal Resources Center, Westlake University, Hangzhou 310024, ChinaWestlake Four-Dimensional Dynamic Metabolomics (Meta4D) Laboratory, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, ChinaWestlake Four-Dimensional Dynamic Metabolomics (Meta4D) Laboratory, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, ChinaNeuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USAWestlake Four-Dimensional Dynamic Metabolomics (Meta4D) Laboratory, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, ChinaCerebrospinal fluid (CSF) is an important sample source for diagnosing diseases in the central nervous system (CNS), but collecting and injecting CSF in small animals is technically challenging and often results in high mortality rates. Here, we present a cost-effective and efficient method for accessing the CSF in live rodents for fluid collection and infusion purposes. The key element of this protocol is a metal needle tool bent at a unique angle and length, allowing the successful access of the CSF through the foramen magnum. With this method, we can collect 5–10 µL of the CSF from mice and 70–100 µL from rats for downstream analyses, including mass spectrometry. Moreover, our minimally-invasive procedure enables iterative CSF collection from the same animal every few days, representing a significant improvement over prior protocols. Additionally, our method can be used to inject solutions into mice cisterna magna with high success rates and high postoperative recovery rates. In summary, we provide an efficient and minimally-invasive protocol for collecting and infusing reagents into the CSF in live rodents. We envision this protocol will facilitate biomarker discovery and drug development for diseases in the central nervous system.https://www.mdpi.com/2227-9059/11/6/1609cerebrospinal fluid (CSF)central nervous system (CNS) diseasesmouse modelsCSF biomarkerCSF collectionCSF injection |
| spellingShingle | Jingrong Regina Han Yu Yang Tianshu William Wu Tao-Tao Shi Wenlu Li Yilong Zou A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates Biomedicines cerebrospinal fluid (CSF) central nervous system (CNS) diseases mouse models CSF biomarker CSF collection CSF injection |
| title | A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates |
| title_full | A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates |
| title_fullStr | A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates |
| title_full_unstemmed | A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates |
| title_short | A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival Rates |
| title_sort | minimally invasive method for serial cerebrospinal fluid collection and injection in rodents with high survival rates |
| topic | cerebrospinal fluid (CSF) central nervous system (CNS) diseases mouse models CSF biomarker CSF collection CSF injection |
| url | https://www.mdpi.com/2227-9059/11/6/1609 |
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