Modeling blood diseases with human induced pluripotent stem cells
Induced pluripotent stem cells (iPSCs) are derived from somatic cells through a reprogramming process, which converts them to a pluripotent state, akin to that of embryonic stem cells. Over the past decade, iPSC models have found increasing applications in the study of human diseases, with blood dis...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2019-06-01
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| Series: | Disease Models & Mechanisms |
| Subjects: | |
| Online Access: | http://dmm.biologists.org/content/12/6/dmm039321 |
| _version_ | 1819102171071774720 |
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| author | Maria Georgomanoli Eirini P. Papapetrou |
| author_facet | Maria Georgomanoli Eirini P. Papapetrou |
| author_sort | Maria Georgomanoli |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) are derived from somatic cells through a reprogramming process, which converts them to a pluripotent state, akin to that of embryonic stem cells. Over the past decade, iPSC models have found increasing applications in the study of human diseases, with blood disorders featuring prominently. Here, we discuss methodological aspects pertaining to iPSC generation, hematopoietic differentiation and gene editing, and provide an overview of uses of iPSCs in modeling the cell and gene therapy of inherited genetic blood disorders, as well as their more recent use as models of myeloid malignancies. We also discuss the strengths and limitations of iPSCs compared to model organisms and other cellular systems commonly used in hematology research. |
| first_indexed | 2024-12-22T01:30:19Z |
| format | Article |
| id | doaj.art-60467ee7f84045dcb64cbcb710761c54 |
| institution | Directory Open Access Journal |
| issn | 1754-8403 1754-8411 |
| language | English |
| last_indexed | 2024-12-22T01:30:19Z |
| publishDate | 2019-06-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Disease Models & Mechanisms |
| spelling | doaj.art-60467ee7f84045dcb64cbcb710761c542022-12-21T18:43:31ZengThe Company of BiologistsDisease Models & Mechanisms1754-84031754-84112019-06-0112610.1242/dmm.039321039321Modeling blood diseases with human induced pluripotent stem cellsMaria Georgomanoli0Eirini P. Papapetrou1 Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Induced pluripotent stem cells (iPSCs) are derived from somatic cells through a reprogramming process, which converts them to a pluripotent state, akin to that of embryonic stem cells. Over the past decade, iPSC models have found increasing applications in the study of human diseases, with blood disorders featuring prominently. Here, we discuss methodological aspects pertaining to iPSC generation, hematopoietic differentiation and gene editing, and provide an overview of uses of iPSCs in modeling the cell and gene therapy of inherited genetic blood disorders, as well as their more recent use as models of myeloid malignancies. We also discuss the strengths and limitations of iPSCs compared to model organisms and other cellular systems commonly used in hematology research.http://dmm.biologists.org/content/12/6/dmm039321iPSC generationHematopoietic differentiationGenetic blood disordersMonogenic disordersMyeloid malignancies |
| spellingShingle | Maria Georgomanoli Eirini P. Papapetrou Modeling blood diseases with human induced pluripotent stem cells Disease Models & Mechanisms iPSC generation Hematopoietic differentiation Genetic blood disorders Monogenic disorders Myeloid malignancies |
| title | Modeling blood diseases with human induced pluripotent stem cells |
| title_full | Modeling blood diseases with human induced pluripotent stem cells |
| title_fullStr | Modeling blood diseases with human induced pluripotent stem cells |
| title_full_unstemmed | Modeling blood diseases with human induced pluripotent stem cells |
| title_short | Modeling blood diseases with human induced pluripotent stem cells |
| title_sort | modeling blood diseases with human induced pluripotent stem cells |
| topic | iPSC generation Hematopoietic differentiation Genetic blood disorders Monogenic disorders Myeloid malignancies |
| url | http://dmm.biologists.org/content/12/6/dmm039321 |
| work_keys_str_mv | AT mariageorgomanoli modelingblooddiseaseswithhumaninducedpluripotentstemcells AT eirinippapapetrou modelingblooddiseaseswithhumaninducedpluripotentstemcells |