A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions
<i>Helichrysum arenarium</i> (L.) Moench (abbrev. as HA) has a long tradition in European ethnomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, <i>Helichrysum italicum</i> (Roth) G. Don (abbrev. as HI) is more co...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/3/380 |
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| author | Katja Kramberger Zala Jenko Pražnikar Alenka Baruca Arbeiter Ana Petelin Dunja Bandelj Saša Kenig |
| author_facet | Katja Kramberger Zala Jenko Pražnikar Alenka Baruca Arbeiter Ana Petelin Dunja Bandelj Saša Kenig |
| author_sort | Katja Kramberger |
| collection | DOAJ |
| description | <i>Helichrysum arenarium</i> (L.) Moench (abbrev. as HA) has a long tradition in European ethnomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, <i>Helichrysum italicum</i> (Roth) G. Don (abbrev. as HI) is more common. Since infusions from both plants are traditionally used, we aimed to compare their antioxidative potential using in vitro assays. Two morphologically distinct HI plants, HIa and HIb, were compared to a commercially available HA product. Genetic analysis using microsatellites confirmed a clear differentiation between HI and HA and suggested that HIb was a hybrid resulting from spontaneous hybridization from unknown HI subspecies. High-performance liquid chromatography–mass spectrometry analysis showed the highest amounts of hydroxycinnamic acids and total arzanol derivatives in HIa, whereas HIb was richest in monohydroxybenzoic acids, caffeic acids, and coumarins, and HA contained the highest amounts of flavonoids, especially flavanones. HIa exhibited the highest radical scavenging activity; it was more efficient in protecting different cell lines from induced oxidative stress and in inducing oxidative stress-related genes superoxide dismutase 1, catalase, and glutathione reductase 1. The antioxidative potential of HI was not only dependent on the morphological type of the plant but also on the harvest date, revealing important information for obtaining the best possible product. Considering the superior properties of HI compared to HA, the evaluation of HI as a medicinal plant could be recommended. |
| first_indexed | 2024-03-09T05:42:10Z |
| format | Article |
| id | doaj.art-60475500968a4e88b3f8d088c465f0f3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T05:42:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-60475500968a4e88b3f8d088c465f0f32023-12-03T12:24:03ZengMDPI AGAntioxidants2076-39212021-03-0110338010.3390/antiox10030380A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> InfusionsKatja Kramberger0Zala Jenko Pražnikar1Alenka Baruca Arbeiter2Ana Petelin3Dunja Bandelj4Saša Kenig5Faculty of Health Sciences, University of Primorska, 6310 Izola, SloveniaFaculty of Health Sciences, University of Primorska, 6310 Izola, SloveniaFaculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, SloveniaFaculty of Health Sciences, University of Primorska, 6310 Izola, SloveniaFaculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, SloveniaFaculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia<i>Helichrysum arenarium</i> (L.) Moench (abbrev. as HA) has a long tradition in European ethnomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, <i>Helichrysum italicum</i> (Roth) G. Don (abbrev. as HI) is more common. Since infusions from both plants are traditionally used, we aimed to compare their antioxidative potential using in vitro assays. Two morphologically distinct HI plants, HIa and HIb, were compared to a commercially available HA product. Genetic analysis using microsatellites confirmed a clear differentiation between HI and HA and suggested that HIb was a hybrid resulting from spontaneous hybridization from unknown HI subspecies. High-performance liquid chromatography–mass spectrometry analysis showed the highest amounts of hydroxycinnamic acids and total arzanol derivatives in HIa, whereas HIb was richest in monohydroxybenzoic acids, caffeic acids, and coumarins, and HA contained the highest amounts of flavonoids, especially flavanones. HIa exhibited the highest radical scavenging activity; it was more efficient in protecting different cell lines from induced oxidative stress and in inducing oxidative stress-related genes superoxide dismutase 1, catalase, and glutathione reductase 1. The antioxidative potential of HI was not only dependent on the morphological type of the plant but also on the harvest date, revealing important information for obtaining the best possible product. Considering the superior properties of HI compared to HA, the evaluation of HI as a medicinal plant could be recommended.https://www.mdpi.com/2076-3921/10/3/380<i>Helichrysum</i>medicinal plantsinfusionsphenolic compoundsantioxidative potential |
| spellingShingle | Katja Kramberger Zala Jenko Pražnikar Alenka Baruca Arbeiter Ana Petelin Dunja Bandelj Saša Kenig A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions Antioxidants <i>Helichrysum</i> medicinal plants infusions phenolic compounds antioxidative potential |
| title | A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions |
| title_full | A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions |
| title_fullStr | A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions |
| title_full_unstemmed | A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions |
| title_short | A Comparative Study of the Antioxidative Effects of <i>Helichrysum italicum</i> and <i>Helichrysum arenarium</i> Infusions |
| title_sort | comparative study of the antioxidative effects of i helichrysum italicum i and i helichrysum arenarium i infusions |
| topic | <i>Helichrysum</i> medicinal plants infusions phenolic compounds antioxidative potential |
| url | https://www.mdpi.com/2076-3921/10/3/380 |
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