Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108
Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 20...
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/2/237 |
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| author | Maksym Myronovskyi Birgit Rosenkränzer Marc Stierhof Lutz Petzke Tobias Seiser Andriy Luzhetskyy |
| author_facet | Maksym Myronovskyi Birgit Rosenkränzer Marc Stierhof Lutz Petzke Tobias Seiser Andriy Luzhetskyy |
| author_sort | Maksym Myronovskyi |
| collection | DOAJ |
| description | Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of <i>Streptomyces albus</i> subsp. <i>chlorinus</i> NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies. |
| first_indexed | 2024-12-23T20:57:53Z |
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| id | doaj.art-604d3dd2870f4837bdcc79ec4860843c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-23T20:57:53Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-604d3dd2870f4837bdcc79ec4860843c2022-12-21T17:31:28ZengMDPI AGMicroorganisms2076-26072020-02-018223710.3390/microorganisms8020237microorganisms8020237Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108Maksym Myronovskyi0Birgit Rosenkränzer1Marc Stierhof2Lutz Petzke3Tobias Seiser4Andriy Luzhetskyy5Pharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, GermanyPharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, GermanyPharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, GermanyBASF SE, 67056 Ludwigshafen, GermanyBASF SE, 67056 Ludwigshafen, GermanyPharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, GermanyHerbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of <i>Streptomyces albus</i> subsp. <i>chlorinus</i> NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies.https://www.mdpi.com/2076-2607/8/2/237albucidinherbicidenucleosidebiosynthetic gene clusterheterologous expression<i>streptomyces albus</i> del14 |
| spellingShingle | Maksym Myronovskyi Birgit Rosenkränzer Marc Stierhof Lutz Petzke Tobias Seiser Andriy Luzhetskyy Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 Microorganisms albucidin herbicide nucleoside biosynthetic gene cluster heterologous expression <i>streptomyces albus</i> del14 |
| title | Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 |
| title_full | Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 |
| title_fullStr | Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 |
| title_full_unstemmed | Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 |
| title_short | Identification and Heterologous Expression of the Albucidin Gene Cluster from the Marine Strain <i>Streptomyces Albus</i> Subsp. <i>Chlorinus</i> NRRL B-24108 |
| title_sort | identification and heterologous expression of the albucidin gene cluster from the marine strain i streptomyces albus i subsp i chlorinus i nrrl b 24108 |
| topic | albucidin herbicide nucleoside biosynthetic gene cluster heterologous expression <i>streptomyces albus</i> del14 |
| url | https://www.mdpi.com/2076-2607/8/2/237 |
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