Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study
Recent findings indicate a significant association between sedentary (SED)-time and type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether different levels of SED-time could impact on biochemical and physiological processes occurring in sedentary and physically inactive T2...
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| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/5/205 |
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| author | Elisa Benetti Erica Liberto Davide Bressanello Valentina Bordano Arianna C. Rosa Gianluca Miglio Jonida Haxhi Giuseppe Pugliese Stefano Balducci Chiara Cordero |
| author_facet | Elisa Benetti Erica Liberto Davide Bressanello Valentina Bordano Arianna C. Rosa Gianluca Miglio Jonida Haxhi Giuseppe Pugliese Stefano Balducci Chiara Cordero |
| author_sort | Elisa Benetti |
| collection | DOAJ |
| description | Recent findings indicate a significant association between sedentary (SED)-time and type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether different levels of SED-time could impact on biochemical and physiological processes occurring in sedentary and physically inactive T2DM patients. In particular, patients from the “Italian Diabetes and Exercise Study (IDES)_2 trial belonging to the first and fourth quartile of SED-time were compared. Urine samples were analyzed by comprehensive two-dimensional gas chromatography (GC × GC) with parallel detection by mass spectrometry and flame ionization detection (GC × 2GC-MS/FID). This platform enables accurate profiling and fingerprinting of urinary metabolites while maximizing the overall information capacity, quantitation reliability, and response linearity. Moreover, using advanced pattern recognition, the fingerprinting process was extended to untargeted and targeted features, revealing diagnostic urinary fingerprints between groups. Quantitative metabolomics was then applied to analytes of relevance for robust comparisons. Increased levels of glycine, L-valine, L-threonine, L-phenylalanine, L-leucine, L-alanine, succinic acid, 2-ketoglutaric acid, xylitol, and ribitol were revealed in samples from less sedentary women. In conclusion, SED-time is associated with changes in urine metabolome signatures. These preliminary results suggest that reducing SED-time could be a strategy to improve the health status of a large proportion of diabetic patients. |
| first_indexed | 2024-03-10T19:45:43Z |
| format | Article |
| id | doaj.art-604fed1da42b436ba0d69637d21e2483 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T19:45:43Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-604fed1da42b436ba0d69637d21e24832023-11-20T00:51:07ZengMDPI AGMetabolites2218-19892020-05-0110520510.3390/metabo10050205Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional StudyElisa Benetti0Erica Liberto1Davide Bressanello2Valentina Bordano3Arianna C. Rosa4Gianluca Miglio5Jonida Haxhi6Giuseppe Pugliese7Stefano Balducci8Chiara Cordero9Department of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyDepartment of Clinical and Molecular Medicine, ‘‘La Sapienza” University, 00183 Rome, ItalyDepartment of Clinical and Molecular Medicine, ‘‘La Sapienza” University, 00183 Rome, ItalyDepartment of Clinical and Molecular Medicine, ‘‘La Sapienza” University, 00183 Rome, ItalyDepartment of Drug Science and Technology University of Turin, 10125 Turin, ItalyRecent findings indicate a significant association between sedentary (SED)-time and type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether different levels of SED-time could impact on biochemical and physiological processes occurring in sedentary and physically inactive T2DM patients. In particular, patients from the “Italian Diabetes and Exercise Study (IDES)_2 trial belonging to the first and fourth quartile of SED-time were compared. Urine samples were analyzed by comprehensive two-dimensional gas chromatography (GC × GC) with parallel detection by mass spectrometry and flame ionization detection (GC × 2GC-MS/FID). This platform enables accurate profiling and fingerprinting of urinary metabolites while maximizing the overall information capacity, quantitation reliability, and response linearity. Moreover, using advanced pattern recognition, the fingerprinting process was extended to untargeted and targeted features, revealing diagnostic urinary fingerprints between groups. Quantitative metabolomics was then applied to analytes of relevance for robust comparisons. Increased levels of glycine, L-valine, L-threonine, L-phenylalanine, L-leucine, L-alanine, succinic acid, 2-ketoglutaric acid, xylitol, and ribitol were revealed in samples from less sedentary women. In conclusion, SED-time is associated with changes in urine metabolome signatures. These preliminary results suggest that reducing SED-time could be a strategy to improve the health status of a large proportion of diabetic patients.https://www.mdpi.com/2218-1989/10/5/205sedentarinessurine metabolometype 2 diabetesobservational studycomprehensive two-dimensional gas chromatographydual parallel detection by mass spectrometry and flame ionization detector |
| spellingShingle | Elisa Benetti Erica Liberto Davide Bressanello Valentina Bordano Arianna C. Rosa Gianluca Miglio Jonida Haxhi Giuseppe Pugliese Stefano Balducci Chiara Cordero Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study Metabolites sedentariness urine metabolome type 2 diabetes observational study comprehensive two-dimensional gas chromatography dual parallel detection by mass spectrometry and flame ionization detector |
| title | Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study |
| title_full | Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study |
| title_fullStr | Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study |
| title_full_unstemmed | Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study |
| title_short | Sedentariness and Urinary Metabolite Profile in Type 2 Diabetic Patients, a Cross-Sectional Study |
| title_sort | sedentariness and urinary metabolite profile in type 2 diabetic patients a cross sectional study |
| topic | sedentariness urine metabolome type 2 diabetes observational study comprehensive two-dimensional gas chromatography dual parallel detection by mass spectrometry and flame ionization detector |
| url | https://www.mdpi.com/2218-1989/10/5/205 |
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