A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species
Summary: Metabarcoding techniques are revolutionizing studies of marine biodiversity. They can be used for monitoring non-indigenous species (NIS) in ports and harbors. However, they are often biased by inconsistent sampling methods and incomplete reference databases. Logistic constraints in ports p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223026652 |
| _version_ | 1827587936521027584 |
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| author | Jesús Zarcero Adrià Antich Marc Rius Owen S. Wangensteen Xavier Turon |
| author_facet | Jesús Zarcero Adrià Antich Marc Rius Owen S. Wangensteen Xavier Turon |
| author_sort | Jesús Zarcero |
| collection | DOAJ |
| description | Summary: Metabarcoding techniques are revolutionizing studies of marine biodiversity. They can be used for monitoring non-indigenous species (NIS) in ports and harbors. However, they are often biased by inconsistent sampling methods and incomplete reference databases. Logistic constraints in ports prompt the development of simple, easy-to-deploy samplers. We tested a new device called polyamide mesh for ports organismal monitoring (POMPOM) with a high surface-to-volume ratio. POMPOMS were deployed inside a fishing and recreational port in the Mediterranean alongside conventional settlement plates. We also compiled a curated database with cytochrome oxidase (COI) sequences of Mediterranean NIS. COI metabarcoding of the communities settled in the POMPOMs captured a similar biodiversity than settlement plates, with shared molecular operational units (MOTUs) representing ca. 99% of reads. 38 NIS were detected in the port accounting for ca. 26% of reads. POMPOMs were easy to deploy and handle and provide an efficient method for NIS surveillance. |
| first_indexed | 2024-03-09T00:25:14Z |
| format | Article |
| id | doaj.art-6050029db02247a3b8d14662a5d656ce |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-09T00:25:14Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6050029db02247a3b8d14662a5d656ce2023-12-12T04:35:22ZengElsevieriScience2589-00422024-01-01271108588A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous speciesJesús Zarcero0Adrià Antich1Marc Rius2Owen S. Wangensteen3Xavier Turon4Department of Marine Ecology, Centre for Advanced Studies of Blanes (CEAB), CSIC, 17300 Blanes, Catalonia, Spain; Department of Evolutionary Biology, Ecology and Environmental Sciences and Biodiversity Research Institute (IRBio), University of Barcelona, 08028 Barcelona, Catalonia, SpainDepartment of Marine Ecology, Centre for Advanced Studies of Blanes (CEAB), CSIC, 17300 Blanes, Catalonia, SpainDepartment of Marine Ecology, Centre for Advanced Studies of Blanes (CEAB), CSIC, 17300 Blanes, Catalonia, Spain; Department of Zoology, Centre for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park Johannesburg 2006, South AfricaDepartment of Evolutionary Biology, Ecology and Environmental Sciences and Biodiversity Research Institute (IRBio), University of Barcelona, 08028 Barcelona, Catalonia, SpainDepartment of Marine Ecology, Centre for Advanced Studies of Blanes (CEAB), CSIC, 17300 Blanes, Catalonia, Spain; Corresponding authorSummary: Metabarcoding techniques are revolutionizing studies of marine biodiversity. They can be used for monitoring non-indigenous species (NIS) in ports and harbors. However, they are often biased by inconsistent sampling methods and incomplete reference databases. Logistic constraints in ports prompt the development of simple, easy-to-deploy samplers. We tested a new device called polyamide mesh for ports organismal monitoring (POMPOM) with a high surface-to-volume ratio. POMPOMS were deployed inside a fishing and recreational port in the Mediterranean alongside conventional settlement plates. We also compiled a curated database with cytochrome oxidase (COI) sequences of Mediterranean NIS. COI metabarcoding of the communities settled in the POMPOMs captured a similar biodiversity than settlement plates, with shared molecular operational units (MOTUs) representing ca. 99% of reads. 38 NIS were detected in the port accounting for ca. 26% of reads. POMPOMs were easy to deploy and handle and provide an efficient method for NIS surveillance.http://www.sciencedirect.com/science/article/pii/S2589004223026652EcologyBiological sciencesEvolutionary biology |
| spellingShingle | Jesús Zarcero Adrià Antich Marc Rius Owen S. Wangensteen Xavier Turon A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species iScience Ecology Biological sciences Evolutionary biology |
| title | A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species |
| title_full | A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species |
| title_fullStr | A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species |
| title_full_unstemmed | A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species |
| title_short | A new sampling device for metabarcoding surveillance of port communities and detection of non-indigenous species |
| title_sort | new sampling device for metabarcoding surveillance of port communities and detection of non indigenous species |
| topic | Ecology Biological sciences Evolutionary biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223026652 |
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