Wool Keratin-Based Nanofibres—In Vitro Validation
Protein-based nanofibres are commonly used in the biomedical field to support cell growth. For this study, the cell viability of wool keratin-based nanofibres was tested. Membranes were obtained by electrospinning using formic acid, hexafluoroisopropanol, and water as solvents. For aqueous solutions...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/12/224 |
| _version_ | 1797506588264628224 |
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| author | Diego Omar Sanchez Ramirez Iriczalli Cruz-Maya Claudia Vineis Vincenzo Guarino Cinzia Tonetti Alessio Varesano |
| author_facet | Diego Omar Sanchez Ramirez Iriczalli Cruz-Maya Claudia Vineis Vincenzo Guarino Cinzia Tonetti Alessio Varesano |
| author_sort | Diego Omar Sanchez Ramirez |
| collection | DOAJ |
| description | Protein-based nanofibres are commonly used in the biomedical field to support cell growth. For this study, the cell viability of wool keratin-based nanofibres was tested. Membranes were obtained by electrospinning using formic acid, hexafluoroisopropanol, and water as solvents. For aqueous solutions, polyethylene oxide blended with keratin was employed, and their use to support in vitro cell interactions was also validated. Morphological characterization and secondary structure quantification were carried out by SEM and FTIR analyses. Although formic acid produced the best nanofibres from a morphological point of view, the results showed a better response to cell proliferation after 14 days in the case of fibres from hexafluoroisopropanol solution. Polyethylene oxide in keratin nanofibres was demonstrated, over time, to influence in vitro cell interactions, modifying membranes-wettability and reducing the contact between keratin chains and water molecules, respectively. |
| first_indexed | 2024-03-10T04:34:41Z |
| format | Article |
| id | doaj.art-6050214830b34f4b8c09139c07c49af0 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T04:34:41Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-6050214830b34f4b8c09139c07c49af02023-11-23T03:52:34ZengMDPI AGBioengineering2306-53542021-12-0181222410.3390/bioengineering8120224Wool Keratin-Based Nanofibres—In Vitro ValidationDiego Omar Sanchez Ramirez0Iriczalli Cruz-Maya1Claudia Vineis2Vincenzo Guarino3Cinzia Tonetti4Alessio Varesano5National Research Council-Institute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (CNR-STIIMA), Corso Giuseppe Pella 16, 13900 Biella, ItalyNational Research Council-Institute for Polymers, Composites and Biomaterials (CNR-IPCB), Mostra d’Oltremare, Pad. 20, V.le J.F. Kennedy 54, 80125 Napoli, ItalyNational Research Council-Institute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (CNR-STIIMA), Corso Giuseppe Pella 16, 13900 Biella, ItalyNational Research Council-Institute for Polymers, Composites and Biomaterials (CNR-IPCB), Mostra d’Oltremare, Pad. 20, V.le J.F. Kennedy 54, 80125 Napoli, ItalyNational Research Council-Institute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (CNR-STIIMA), Corso Giuseppe Pella 16, 13900 Biella, ItalyNational Research Council-Institute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (CNR-STIIMA), Corso Giuseppe Pella 16, 13900 Biella, ItalyProtein-based nanofibres are commonly used in the biomedical field to support cell growth. For this study, the cell viability of wool keratin-based nanofibres was tested. Membranes were obtained by electrospinning using formic acid, hexafluoroisopropanol, and water as solvents. For aqueous solutions, polyethylene oxide blended with keratin was employed, and their use to support in vitro cell interactions was also validated. Morphological characterization and secondary structure quantification were carried out by SEM and FTIR analyses. Although formic acid produced the best nanofibres from a morphological point of view, the results showed a better response to cell proliferation after 14 days in the case of fibres from hexafluoroisopropanol solution. Polyethylene oxide in keratin nanofibres was demonstrated, over time, to influence in vitro cell interactions, modifying membranes-wettability and reducing the contact between keratin chains and water molecules, respectively.https://www.mdpi.com/2306-5354/8/12/224wool-keratinelectrospinningcell growthscaffolds |
| spellingShingle | Diego Omar Sanchez Ramirez Iriczalli Cruz-Maya Claudia Vineis Vincenzo Guarino Cinzia Tonetti Alessio Varesano Wool Keratin-Based Nanofibres—In Vitro Validation Bioengineering wool-keratin electrospinning cell growth scaffolds |
| title | Wool Keratin-Based Nanofibres—In Vitro Validation |
| title_full | Wool Keratin-Based Nanofibres—In Vitro Validation |
| title_fullStr | Wool Keratin-Based Nanofibres—In Vitro Validation |
| title_full_unstemmed | Wool Keratin-Based Nanofibres—In Vitro Validation |
| title_short | Wool Keratin-Based Nanofibres—In Vitro Validation |
| title_sort | wool keratin based nanofibres in vitro validation |
| topic | wool-keratin electrospinning cell growth scaffolds |
| url | https://www.mdpi.com/2306-5354/8/12/224 |
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