Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica
Abstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical met...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1576-7 |
| _version_ | 1818264529369300992 |
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| author | Pratik Prashant Pawar Annamma Anil Odaneth Rajeshkumar Natwarlal Vadgama Arvind Mallinath Lali |
| author_facet | Pratik Prashant Pawar Annamma Anil Odaneth Rajeshkumar Natwarlal Vadgama Arvind Mallinath Lali |
| author_sort | Pratik Prashant Pawar |
| collection | DOAJ |
| description | Abstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory. |
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| format | Article |
| id | doaj.art-6053f1d1c25347dbb8a6fd05b258e3e8 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T19:36:22Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-6053f1d1c25347dbb8a6fd05b258e3e82022-12-22T00:14:18ZengBMCBiotechnology for Biofuels1754-68342019-10-0112111810.1186/s13068-019-1576-7Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolyticaPratik Prashant Pawar0Annamma Anil Odaneth1Rajeshkumar Natwarlal Vadgama2Arvind Mallinath Lali3DBT-ICT Centre for Energy Biosciences, Institute of Chemical TechnologyDBT-ICT Centre for Energy Biosciences, Institute of Chemical TechnologyDBT-ICT Centre for Energy Biosciences, Institute of Chemical TechnologyDBT-ICT Centre for Energy Biosciences, Institute of Chemical TechnologyAbstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory.http://link.springer.com/article/10.1186/s13068-019-1576-7Microbial oilYarrowia lipolyticaContinuous productionOil capturing agent (OCA)Extractive production |
| spellingShingle | Pratik Prashant Pawar Annamma Anil Odaneth Rajeshkumar Natwarlal Vadgama Arvind Mallinath Lali Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica Biotechnology for Biofuels Microbial oil Yarrowia lipolytica Continuous production Oil capturing agent (OCA) Extractive production |
| title | Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica |
| title_full | Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica |
| title_fullStr | Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica |
| title_full_unstemmed | Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica |
| title_short | Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica |
| title_sort | simultaneous lipid biosynthesis and recovery for oleaginous yeast yarrowia lipolytica |
| topic | Microbial oil Yarrowia lipolytica Continuous production Oil capturing agent (OCA) Extractive production |
| url | http://link.springer.com/article/10.1186/s13068-019-1576-7 |
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