Application of ionising radiation for the stabilisation of Trichoderma viride cellulases
The solutions of cellulolytic enzymes designated as standards for the cellulase activity assay were exposed in sealed glass ampoules (containing at least 100 Cx-units per ml in 30% w/w glycerol) to gamma radiation within the dose interval of 0-18 kGy. Glycerol was found to be the best enzyme stabili...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-06-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0004_application-of-ionising-radiation-for-the-stabilisation-of-trichoderma-viride-cellulases.php |
| _version_ | 1797899880497152000 |
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| author | Vít Plaček Karel Vacek Jan Káš Kateřina Demnerová Jarmila Zídková Jiří Sajdok |
| author_facet | Vít Plaček Karel Vacek Jan Káš Kateřina Demnerová Jarmila Zídková Jiří Sajdok |
| author_sort | Vít Plaček |
| collection | DOAJ |
| description | The solutions of cellulolytic enzymes designated as standards for the cellulase activity assay were exposed in sealed glass ampoules (containing at least 100 Cx-units per ml in 30% w/w glycerol) to gamma radiation within the dose interval of 0-18 kGy. Glycerol was found to be the best enzyme stabiliser, however, the dose for the decontamination had to be increased in comparison with the original solution because glycerol protected also the contaminating microflora. The preparation after such treatment (30% of glycerol, dose 7 kGy) retained about 95% of the initial enzymatic activity without any decrease taking place in the following 6 months. The loss of the side activities did not exceed 10.5% and no bacterial contamination was detected either after 6 months of storage following the irradiation. No difference was found in the immunoreactivity of cellulases or in protein chromatografic (FPLC) pattern between the original and the irradiated enzyme preparations. |
| first_indexed | 2024-04-10T08:35:51Z |
| format | Article |
| id | doaj.art-60547dc25681452fa5e989f7d58f75ae |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:51Z |
| publishDate | 2005-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-60547dc25681452fa5e989f7d58f75ae2023-02-23T03:26:58ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172005-06-0123311111510.17221/3379-CJFScjf-200503-0004Application of ionising radiation for the stabilisation of Trichoderma viride cellulasesVít Plaček0Karel Vacek1Jan Káš2Kateřina Demnerová3Jarmila Zídková4Jiří Sajdok5Department of Radiation Applications, Nuclear Research Institute, Řež, Czech RepublicDepartment of Radiation Applications, Nuclear Research Institute, Řež, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech RepublicThe solutions of cellulolytic enzymes designated as standards for the cellulase activity assay were exposed in sealed glass ampoules (containing at least 100 Cx-units per ml in 30% w/w glycerol) to gamma radiation within the dose interval of 0-18 kGy. Glycerol was found to be the best enzyme stabiliser, however, the dose for the decontamination had to be increased in comparison with the original solution because glycerol protected also the contaminating microflora. The preparation after such treatment (30% of glycerol, dose 7 kGy) retained about 95% of the initial enzymatic activity without any decrease taking place in the following 6 months. The loss of the side activities did not exceed 10.5% and no bacterial contamination was detected either after 6 months of storage following the irradiation. No difference was found in the immunoreactivity of cellulases or in protein chromatografic (FPLC) pattern between the original and the irradiated enzyme preparations.https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0004_application-of-ionising-radiation-for-the-stabilisation-of-trichoderma-viride-cellulases.phpcellulase enzymestabilisationgamma irradiation |
| spellingShingle | Vít Plaček Karel Vacek Jan Káš Kateřina Demnerová Jarmila Zídková Jiří Sajdok Application of ionising radiation for the stabilisation of Trichoderma viride cellulases Czech Journal of Food Sciences cellulase enzyme stabilisation gamma irradiation |
| title | Application of ionising radiation for the stabilisation of Trichoderma viride cellulases |
| title_full | Application of ionising radiation for the stabilisation of Trichoderma viride cellulases |
| title_fullStr | Application of ionising radiation for the stabilisation of Trichoderma viride cellulases |
| title_full_unstemmed | Application of ionising radiation for the stabilisation of Trichoderma viride cellulases |
| title_short | Application of ionising radiation for the stabilisation of Trichoderma viride cellulases |
| title_sort | application of ionising radiation for the stabilisation of trichoderma viride cellulases |
| topic | cellulase enzyme stabilisation gamma irradiation |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200503-0004_application-of-ionising-radiation-for-the-stabilisation-of-trichoderma-viride-cellulases.php |
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