Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis
<i>Background and Objectives</i>: Most published research has only investigated a single timepoint after the onset of severe acute pancreatitis (SAP), meaning that they have been unable to observe the relationship between the dynamic changes in cytokines and SAP progression. In this stud...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/1648-9144/59/2/321 |
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| author | Rui Zhou Wangjun Bu Yudan Fan Ziwei Du Jian Zhang Shu Zhang Jin Sun Zongfang Li Jun Li |
| author_facet | Rui Zhou Wangjun Bu Yudan Fan Ziwei Du Jian Zhang Shu Zhang Jin Sun Zongfang Li Jun Li |
| author_sort | Rui Zhou |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Most published research has only investigated a single timepoint after the onset of severe acute pancreatitis (SAP), meaning that they have been unable to observe the relationship between the dynamic changes in cytokines and SAP progression. In this study, we attempted to reveal the relationship between dynamic changes in cytokine expression levels and SAP disease progression and the relationship between cytokines, using continuous large-scale cytokine detection. <i>Materials and Methods</i>: Seventy rats were randomly assigned to control (Con), sham operation (SO) and SAP groups. The SAP group was randomly allocated to five subgroups at 3, 6, 9, 12 and 15 h after the operation. In the SAP group, 5% sodium taurocholate was injected retrograde into the pancreatic bile duct. Animals in the SO group received a similar incision, a turning over of the pancreas. Control animals did not receive any treatment. We observed the survival, ascites fluid amount, pancreatic histopathological scores and serum amylase activity of SAP rats. We used the cytokine microarray to simultaneously detect 90 cytokines and the dynamic changes in one experiment and to analyze the correlation between cytokine expression and disease progression. <i>Results</i>: The mortality of SAP rats increased with an increase in time. Serum amylase activity, pancreatic histopathological scores and ascites fluid amount were time-dependent. Compared with normal rats, 69 cytokines in SAP rats were significantly changed for at least one timepoint, and 49 cytokines were significantly changed at different timepoints after SAP induction. The changes in inflammatory cytokines were significantly upregulated at 6 and 9 h and 12 h and then significantly decreased. <i>Conclusions</i>: The trend of cytokine expression in SAP rats was not consistent with the disease progression. The cytokine–cytokine receptor interaction and MAPK signal’s dominant cytokines were always highly expressed at various time points over the course of SAP. |
| first_indexed | 2024-03-11T08:27:16Z |
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| last_indexed | 2024-03-11T08:27:16Z |
| publishDate | 2023-02-01 |
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| series | Medicina |
| spelling | doaj.art-6059783f9e664ea7b57288792f23faaa2023-11-16T22:00:11ZengMDPI AGMedicina1010-660X1648-91442023-02-0159232110.3390/medicina59020321Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute PancreatitisRui Zhou0Wangjun Bu1Yudan Fan2Ziwei Du3Jian Zhang4Shu Zhang5Jin Sun6Zongfang Li7Jun Li8National & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaDepartment of Breast and Thyroid Surgery, The Northwest Women’s and Children’s Hospital, Xi’an 710061, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaNational & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China<i>Background and Objectives</i>: Most published research has only investigated a single timepoint after the onset of severe acute pancreatitis (SAP), meaning that they have been unable to observe the relationship between the dynamic changes in cytokines and SAP progression. In this study, we attempted to reveal the relationship between dynamic changes in cytokine expression levels and SAP disease progression and the relationship between cytokines, using continuous large-scale cytokine detection. <i>Materials and Methods</i>: Seventy rats were randomly assigned to control (Con), sham operation (SO) and SAP groups. The SAP group was randomly allocated to five subgroups at 3, 6, 9, 12 and 15 h after the operation. In the SAP group, 5% sodium taurocholate was injected retrograde into the pancreatic bile duct. Animals in the SO group received a similar incision, a turning over of the pancreas. Control animals did not receive any treatment. We observed the survival, ascites fluid amount, pancreatic histopathological scores and serum amylase activity of SAP rats. We used the cytokine microarray to simultaneously detect 90 cytokines and the dynamic changes in one experiment and to analyze the correlation between cytokine expression and disease progression. <i>Results</i>: The mortality of SAP rats increased with an increase in time. Serum amylase activity, pancreatic histopathological scores and ascites fluid amount were time-dependent. Compared with normal rats, 69 cytokines in SAP rats were significantly changed for at least one timepoint, and 49 cytokines were significantly changed at different timepoints after SAP induction. The changes in inflammatory cytokines were significantly upregulated at 6 and 9 h and 12 h and then significantly decreased. <i>Conclusions</i>: The trend of cytokine expression in SAP rats was not consistent with the disease progression. The cytokine–cytokine receptor interaction and MAPK signal’s dominant cytokines were always highly expressed at various time points over the course of SAP.https://www.mdpi.com/1648-9144/59/2/321severe acute pancreatitiscytokinesinflammationdisease progressionmicroarray |
| spellingShingle | Rui Zhou Wangjun Bu Yudan Fan Ziwei Du Jian Zhang Shu Zhang Jin Sun Zongfang Li Jun Li Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis Medicina severe acute pancreatitis cytokines inflammation disease progression microarray |
| title | Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis |
| title_full | Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis |
| title_fullStr | Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis |
| title_full_unstemmed | Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis |
| title_short | Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis |
| title_sort | dynamic changes in serum cytokine profile in rats with severe acute pancreatitis |
| topic | severe acute pancreatitis cytokines inflammation disease progression microarray |
| url | https://www.mdpi.com/1648-9144/59/2/321 |
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