High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense
Raquel Rangel Silvares1,*, Evelyn Nunes Goulart da Silva Pereira1,*, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cru...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-09-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Subjects: | |
| Online Access: | https://www.dovepress.com/high-fat-diet-induced-kidney-alterations-in-rats-with-metabolic-syndro-peer-reviewed-article-DMSO |
| _version_ | 1797935315824934912 |
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| author | Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A |
| author_facet | Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A |
| author_sort | Rangel Silvares R |
| collection | DOAJ |
| description | Raquel Rangel Silvares1,*, Evelyn Nunes Goulart da Silva Pereira1,*, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 2Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 3Laboratory of Immunopharmacology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil*These authors contributed equally to this workCorrespondence: Anissa DaliryInstituto Oswaldo Cruz, Fiocruz, Pavilhão Ozório de Almeida, Manguinhos, Rio de Janeiro, CEP: 21.040-360, RJ, BrazilEmail daliry@ioc.fiocruz.brIntroduction: This study aimed to investigate changes in renal function and the AGE-RAGE axis in the kidney of a non-genetic animal model of metabolic syndrome (MetS) induced by high-fat diet (HFD). Additionally, we evaluated the protective effect of pyridoxamine (PM), a vitamin B6 analog with anti-AGE effects, in the context of diet-related renal endothelial dysfunction.Methodology: In Wistar rats, the MetS animal model was induced by 20 or 28 weeks of HFD feeding. When indicated, a subgroup of animals was treated daily with PM (60 mg/kg) for 2 months. Tissue perfusion in renal microcirculation was examined by laser speckle contrast imaging. Oxidative stress was analyzed by thiobarbituric acid reactive species and the inflammatory markers by ELISA (TNF-α and IL-1β). Reverse transcription polymerase chain reaction was used to analyze eNOs, IL-6, vascular cell adhesion molecule (VCAM), NADPH oxidase subunit 47 (N47), catalase, and receptor for AGE (RAGE) gene expression.Results: Wistar rats fed a HFD showed negligible alteration in renal function, decrease in catalase mRNA transcripts and catalase enzyme activity compared to control (CTL) animals. Increased levels of IL-1β were observed in the kidney of MetS-induced rats. HFD-fed rats exhibited kidney endothelial dysfunction, with no significant differences in basal microvascular blood flow. PM significantly improved kidney vasorelaxation in HFD-fed rats. eNOS, VCAM, and RAGE gene expression and AGE content were not altered in kidneys of HFD-induced MetS rats in comparison to CTLs.Conclusions: Our findings suggest that HFD-induced microvascular dysfunction precedes the decline in renal function, and could be related to antioxidant machinery defects and inflammation activation in the kidney. PM showed a vasoprotective effect, and thus, could be an important contributory factor in ameliorating diet-induced renal damage.Keywords: metabolic syndrome, kidney endothelial dysfunction, pyridoxamine, advanced glycation end products |
| first_indexed | 2024-04-10T18:12:33Z |
| format | Article |
| id | doaj.art-605b442423bd452da7f8a6a54ce3800f |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:12:33Z |
| publishDate | 2019-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-605b442423bd452da7f8a6a54ce3800f2023-02-02T10:20:54ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072019-09-01Volume 121773178148432High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defenseRangel Silvares RNunes Goulart da Silva Pereira EEduardo Ilaquita Flores ELino Rodrigues KRibeiro Silva AGonçalves-de-Albuquerque CFDaliry ARaquel Rangel Silvares1,*, Evelyn Nunes Goulart da Silva Pereira1,*, Edgar Eduardo Ilaquita Flores1, Karine Lino Rodrigues1, Adriana Ribeiro Silva2, Cassiano Felipe Gonçalves-de-Albuquerque2,3, Anissa Daliry1 1Laboratory of Cardiovascular Investigation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 2Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil; 3Laboratory of Immunopharmacology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil*These authors contributed equally to this workCorrespondence: Anissa DaliryInstituto Oswaldo Cruz, Fiocruz, Pavilhão Ozório de Almeida, Manguinhos, Rio de Janeiro, CEP: 21.040-360, RJ, BrazilEmail daliry@ioc.fiocruz.brIntroduction: This study aimed to investigate changes in renal function and the AGE-RAGE axis in the kidney of a non-genetic animal model of metabolic syndrome (MetS) induced by high-fat diet (HFD). Additionally, we evaluated the protective effect of pyridoxamine (PM), a vitamin B6 analog with anti-AGE effects, in the context of diet-related renal endothelial dysfunction.Methodology: In Wistar rats, the MetS animal model was induced by 20 or 28 weeks of HFD feeding. When indicated, a subgroup of animals was treated daily with PM (60 mg/kg) for 2 months. Tissue perfusion in renal microcirculation was examined by laser speckle contrast imaging. Oxidative stress was analyzed by thiobarbituric acid reactive species and the inflammatory markers by ELISA (TNF-α and IL-1β). Reverse transcription polymerase chain reaction was used to analyze eNOs, IL-6, vascular cell adhesion molecule (VCAM), NADPH oxidase subunit 47 (N47), catalase, and receptor for AGE (RAGE) gene expression.Results: Wistar rats fed a HFD showed negligible alteration in renal function, decrease in catalase mRNA transcripts and catalase enzyme activity compared to control (CTL) animals. Increased levels of IL-1β were observed in the kidney of MetS-induced rats. HFD-fed rats exhibited kidney endothelial dysfunction, with no significant differences in basal microvascular blood flow. PM significantly improved kidney vasorelaxation in HFD-fed rats. eNOS, VCAM, and RAGE gene expression and AGE content were not altered in kidneys of HFD-induced MetS rats in comparison to CTLs.Conclusions: Our findings suggest that HFD-induced microvascular dysfunction precedes the decline in renal function, and could be related to antioxidant machinery defects and inflammation activation in the kidney. PM showed a vasoprotective effect, and thus, could be an important contributory factor in ameliorating diet-induced renal damage.Keywords: metabolic syndrome, kidney endothelial dysfunction, pyridoxamine, advanced glycation end productshttps://www.dovepress.com/high-fat-diet-induced-kidney-alterations-in-rats-with-metabolic-syndro-peer-reviewed-article-DMSOMetabolic syndromekidney endothelial dysfunctionpyridoxamineadvanced glycation end products. |
| spellingShingle | Rangel Silvares R Nunes Goulart da Silva Pereira E Eduardo Ilaquita Flores E Lino Rodrigues K Ribeiro Silva A Gonçalves-de-Albuquerque CF Daliry A High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense Diabetes, Metabolic Syndrome and Obesity Metabolic syndrome kidney endothelial dysfunction pyridoxamine advanced glycation end products. |
| title | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense |
| title_full | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense |
| title_fullStr | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense |
| title_full_unstemmed | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense |
| title_short | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense |
| title_sort | high fat diet induced kidney alterations in rats with metabolic syndrome endothelial dysfunction and decreased antioxidant defense |
| topic | Metabolic syndrome kidney endothelial dysfunction pyridoxamine advanced glycation end products. |
| url | https://www.dovepress.com/high-fat-diet-induced-kidney-alterations-in-rats-with-metabolic-syndro-peer-reviewed-article-DMSO |
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