The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG

Twenty cattles were used in this experiment to determine the quantity and quality of embryo after injected FSH (follicle stimulating hormone ) and PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and...

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Main Authors: Adriani Adriani, B Rosadi, Depison
Format: Article
Language:English
Published: Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science 2009-05-01
Series:Animal Production: Indonesian Journal of Animal Production
Subjects:
Online Access:http://www.animalproduction.net/index.php/JAP/article/view/229
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author Adriani Adriani
B Rosadi
Depison
author_facet Adriani Adriani
B Rosadi
Depison
author_sort Adriani Adriani
collection DOAJ
description Twenty cattles were used in this experiment to determine the quantity and quality of embryo after injected FSH (follicle stimulating hormone ) and PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and 4 replications. The treatments were T1 = 4 mg of FSH twice a day intra-ovary decreased doses, T2 = 8 mg of FSH twice a day intra-ovary decreased doses, T3 = 300 IU of PMSG single dose intra-ovary, T4 = 600 IU of PMSG single dose intra-ovary, T5 = 40 mg of FSH twice a day intramuscular decreased doses. Trial cattle were oestrus synchronized using 15 mg of PGF2α that gave twice at 11-daily intervals. One day after giving FSH and PMSG was detected the cattle’s oestrus. Washing uterus was done at day 7 after AI using mixture of PBS, FCS and streptomicyn. Data observed were cow performances, embryo quantity and embryo quality. Results of experiment showed that 19 cattle (95%) responded oestrus synchronized treatment and super ovulation, whereas 1 cattle (5%) did not respond oestrus synchronized treatment and super ovulation. Generally, cattle showed oestrus at 2 – 3 days after giving PGF2α. Eleven cattle (57,90%) showed oestrus at 2 days after giving PGF2α whereas the others (8 cattle, 42,10%) showed oestrus 3 days giving PGF2α. The treatment of giving FSH and PMSG could increase (P<0,05) embryo. T5 was highest compared the others ( T1, T2, T3 and T4), while T2 and T4 were higher than T1 and T3. Produced total embryo was 82 with average 4,3 ± 5,67 using FSH and PMSG. 8 embryo (9,76%), 9 embryos (10,90), 20 embryo (24,40%), 16 embryo (19,50%) and 29 embryos (35,40%) were grade A, B, C, D and E respectively. It is concluded that giving of 40 mg FSH intramusculer produce the best embryo donor whereas and giving of FSH 8 mg intraovari was the best effeciency. (Animal Production 11(2): 96-102 (2009) Key Words : Brahman Cross Cattle, embryo, PGF2α PMSG, FSH
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spelling doaj.art-605bfa40277e41afb6792f63ed7d439a2024-04-02T23:47:37ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production1411-20272009-05-0111296102The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSGAdriani AdrianiB RosadiDepisonTwenty cattles were used in this experiment to determine the quantity and quality of embryo after injected FSH (follicle stimulating hormone ) and PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and 4 replications. The treatments were T1 = 4 mg of FSH twice a day intra-ovary decreased doses, T2 = 8 mg of FSH twice a day intra-ovary decreased doses, T3 = 300 IU of PMSG single dose intra-ovary, T4 = 600 IU of PMSG single dose intra-ovary, T5 = 40 mg of FSH twice a day intramuscular decreased doses. Trial cattle were oestrus synchronized using 15 mg of PGF2α that gave twice at 11-daily intervals. One day after giving FSH and PMSG was detected the cattle’s oestrus. Washing uterus was done at day 7 after AI using mixture of PBS, FCS and streptomicyn. Data observed were cow performances, embryo quantity and embryo quality. Results of experiment showed that 19 cattle (95%) responded oestrus synchronized treatment and super ovulation, whereas 1 cattle (5%) did not respond oestrus synchronized treatment and super ovulation. Generally, cattle showed oestrus at 2 – 3 days after giving PGF2α. Eleven cattle (57,90%) showed oestrus at 2 days after giving PGF2α whereas the others (8 cattle, 42,10%) showed oestrus 3 days giving PGF2α. The treatment of giving FSH and PMSG could increase (P<0,05) embryo. T5 was highest compared the others ( T1, T2, T3 and T4), while T2 and T4 were higher than T1 and T3. Produced total embryo was 82 with average 4,3 ± 5,67 using FSH and PMSG. 8 embryo (9,76%), 9 embryos (10,90), 20 embryo (24,40%), 16 embryo (19,50%) and 29 embryos (35,40%) were grade A, B, C, D and E respectively. It is concluded that giving of 40 mg FSH intramusculer produce the best embryo donor whereas and giving of FSH 8 mg intraovari was the best effeciency. (Animal Production 11(2): 96-102 (2009) Key Words : Brahman Cross Cattle, embryo, PGF2α PMSG, FSHhttp://www.animalproduction.net/index.php/JAP/article/view/229Brahman Cross CattleembryoPGF2α PMSGFSH
spellingShingle Adriani Adriani
B Rosadi
Depison
The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
Animal Production: Indonesian Journal of Animal Production
Brahman Cross Cattle
embryo
PGF2α PMSG
FSH
title The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
title_full The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
title_fullStr The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
title_full_unstemmed The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
title_short The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG
title_sort quantity and quality of brahman cross cattle embryo after injected fsh and pmsg
topic Brahman Cross Cattle
embryo
PGF2α PMSG
FSH
url http://www.animalproduction.net/index.php/JAP/article/view/229
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