Individual faecal and boot swab sampling to determine John's disease status in small cattle herds
Individual faecal samples were collected from adult animals in 275 cattle farms previously positive for Mycobacterium avium subsp. paratuberculosis (MAP). In addition, boot swab samples were collected in 30 randomly chosen farms. Faecal samples were tested for MAP by a combination of bacterial cultu...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2021-05-01
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| Series: | Veterinaria Italiana |
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| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1389 |
| _version_ | 1818888156168060928 |
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| author | Sophie Gschaider Judith Köchler Joachim Spergser Alexander Tichy Christian Mader Matthias Vill Paul Ortner Josef Kössler Johannes Lorenz Khol |
| author_facet | Sophie Gschaider Judith Köchler Joachim Spergser Alexander Tichy Christian Mader Matthias Vill Paul Ortner Josef Kössler Johannes Lorenz Khol |
| author_sort | Sophie Gschaider |
| collection | DOAJ |
| description | Individual faecal samples were collected from adult animals in 275 cattle farms previously positive for Mycobacterium avium subsp. paratuberculosis (MAP). In addition, boot swab samples were collected in 30 randomly chosen farms. Faecal samples were tested for MAP by a combination of bacterial culture and PCR. A logistic regression and the Pearson Correlation were used to calculate the relation between the number of MAP‑positive cows and boot swab results. In 66.9% of all previously tested herds, no positive individual faecal sample was detected, indicating possible fadeout of the infection. In 9 (30.0%) of the 30 selected farms, at least one MAP‑shedding animal was detected in faecal samples individually collected, while only 5 (16.7%) of these farms were found positive when the boot sampling method was used. The sensitivity of the boot swab sampling increased up to 92% (95% CI: 41%‑99%), if at least 12 animals were faecal MAP‑shedders in a herd. The current study shows possible fadeout of JD in a substantial percentage of previously infected herds. Furthermore, in small herds, a relatively high within‑herd prevalence of MAP‑shedding animals is needed to assure reliable positive boot swab results. |
| first_indexed | 2024-12-19T16:48:38Z |
| format | Article |
| id | doaj.art-605e125cca804264b28acf939ff286d6 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-19T16:48:38Z |
| publishDate | 2021-05-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-605e125cca804264b28acf939ff286d62022-12-21T20:13:36ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272021-05-0157110.12834/VetIt.1389.7584.2Individual faecal and boot swab sampling to determine John's disease status in small cattle herdsSophie Gschaider0Judith Köchler1Joachim Spergser2Alexander Tichy3Christian Mader4Matthias Vill5Paul Ortner6Josef Kössler7Johannes Lorenz Khol8University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine ViennaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine ViennaInstitute of Microbiology, Department for Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaBioinformatics and Biostatistics Platform, Department for Biomedical Sciences University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaVeterinary Health Service Tyrol, Wilhelm-Greil-Straße 17, 6020 Innsbruck, AustriaRegional Veterinary Office Tyrol, Wilhelm-Greil-Straße 17, 6020 Innsbruck, Austria.Regional Veterinary Office Tyrol, Wilhelm-Greil-Straße 17, 6020 Innsbruck, Austria.Regional Veterinary Office Tyrol, Wilhelm-Greil-Straße 17, 6020 Innsbruck, Austria.University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine ViennaIndividual faecal samples were collected from adult animals in 275 cattle farms previously positive for Mycobacterium avium subsp. paratuberculosis (MAP). In addition, boot swab samples were collected in 30 randomly chosen farms. Faecal samples were tested for MAP by a combination of bacterial culture and PCR. A logistic regression and the Pearson Correlation were used to calculate the relation between the number of MAP‑positive cows and boot swab results. In 66.9% of all previously tested herds, no positive individual faecal sample was detected, indicating possible fadeout of the infection. In 9 (30.0%) of the 30 selected farms, at least one MAP‑shedding animal was detected in faecal samples individually collected, while only 5 (16.7%) of these farms were found positive when the boot sampling method was used. The sensitivity of the boot swab sampling increased up to 92% (95% CI: 41%‑99%), if at least 12 animals were faecal MAP‑shedders in a herd. The current study shows possible fadeout of JD in a substantial percentage of previously infected herds. Furthermore, in small herds, a relatively high within‑herd prevalence of MAP‑shedding animals is needed to assure reliable positive boot swab results.https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1389Boot swabCattleFaecal sheddingJohne’s diseaseMycobacterium aviumsubsp. paratuberculosis |
| spellingShingle | Sophie Gschaider Judith Köchler Joachim Spergser Alexander Tichy Christian Mader Matthias Vill Paul Ortner Josef Kössler Johannes Lorenz Khol Individual faecal and boot swab sampling to determine John's disease status in small cattle herds Veterinaria Italiana Boot swab Cattle Faecal shedding Johne’s disease Mycobacterium aviumsubsp. paratuberculosis |
| title | Individual faecal and boot swab sampling to determine John's disease status in small cattle herds |
| title_full | Individual faecal and boot swab sampling to determine John's disease status in small cattle herds |
| title_fullStr | Individual faecal and boot swab sampling to determine John's disease status in small cattle herds |
| title_full_unstemmed | Individual faecal and boot swab sampling to determine John's disease status in small cattle herds |
| title_short | Individual faecal and boot swab sampling to determine John's disease status in small cattle herds |
| title_sort | individual faecal and boot swab sampling to determine john s disease status in small cattle herds |
| topic | Boot swab Cattle Faecal shedding Johne’s disease Mycobacterium aviumsubsp. paratuberculosis |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1389 |
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