Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling
Background/Aims: Autophagy is a cellular degradation process for the recycling of damaged or superfluous intracellular compartments to provide an alternative energy source during periods of metabolic stress for maintaining cell homeostasis and viability. Although autophagy in different contexts have...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2015-01-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/369749 |
| _version_ | 1818216455910457344 |
|---|---|
| author | Rongkai Lin Jiayu Feng Shaoliang Dong Runyang Pan Huiqiang Zhuang Zhenyu Ding |
| author_facet | Rongkai Lin Jiayu Feng Shaoliang Dong Runyang Pan Huiqiang Zhuang Zhenyu Ding |
| author_sort | Rongkai Lin |
| collection | DOAJ |
| description | Background/Aims: Autophagy is a cellular degradation process for the recycling of damaged or superfluous intracellular compartments to provide an alternative energy source during periods of metabolic stress for maintaining cell homeostasis and viability. Although autophagy in different contexts have been shown to use similar signaling pathways, the exact molecular regulation of autophagy has been found to be cell-type dependent. Methods: We used rapamycin to trigger autophagy and used nitric oxide (NO) to inhibit autophagy in prostate cancer cells. IWP-2 was used to inhibit β-catenin signaling. Autophagy-associated proteins were examined by Western blot. Results: We found that nitric oxide (NO), a potent cellular messenger, impaired rapamycin-induced autophagy in prostate cancer cells. Further analyses showed that NO induced nuclear accumulation of β-catenin, a key factor of Wnt signaling pathway, to inhibit autophagy in prostate cancer cells. Conclusions: We demonstrate involvement of β-catenin signaling in the regulation of autophagy of prostate cancer cells. Our results shed light on a previously unappreciated β-catenin signaling pathway for regulating autophagy in prostate cancer. |
| first_indexed | 2024-12-12T06:52:15Z |
| format | Article |
| id | doaj.art-605e2f37d324483e836b47aef7e74f51 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-12T06:52:15Z |
| publishDate | 2015-01-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-605e2f37d324483e836b47aef7e74f512022-12-22T00:34:02ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-01-0135392693210.1159/000369749369749Regulation of Autophagy of Prostate Cancer Cells by β-Catenin SignalingRongkai LinJiayu FengShaoliang DongRunyang PanHuiqiang ZhuangZhenyu DingBackground/Aims: Autophagy is a cellular degradation process for the recycling of damaged or superfluous intracellular compartments to provide an alternative energy source during periods of metabolic stress for maintaining cell homeostasis and viability. Although autophagy in different contexts have been shown to use similar signaling pathways, the exact molecular regulation of autophagy has been found to be cell-type dependent. Methods: We used rapamycin to trigger autophagy and used nitric oxide (NO) to inhibit autophagy in prostate cancer cells. IWP-2 was used to inhibit β-catenin signaling. Autophagy-associated proteins were examined by Western blot. Results: We found that nitric oxide (NO), a potent cellular messenger, impaired rapamycin-induced autophagy in prostate cancer cells. Further analyses showed that NO induced nuclear accumulation of β-catenin, a key factor of Wnt signaling pathway, to inhibit autophagy in prostate cancer cells. Conclusions: We demonstrate involvement of β-catenin signaling in the regulation of autophagy of prostate cancer cells. Our results shed light on a previously unappreciated β-catenin signaling pathway for regulating autophagy in prostate cancer.http://www.karger.com/Article/FullText/369749AutophagyNitric oxideβ-cateninWntProstate cancer |
| spellingShingle | Rongkai Lin Jiayu Feng Shaoliang Dong Runyang Pan Huiqiang Zhuang Zhenyu Ding Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling Cellular Physiology and Biochemistry Autophagy Nitric oxide β-catenin Wnt Prostate cancer |
| title | Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling |
| title_full | Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling |
| title_fullStr | Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling |
| title_full_unstemmed | Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling |
| title_short | Regulation of Autophagy of Prostate Cancer Cells by β-Catenin Signaling |
| title_sort | regulation of autophagy of prostate cancer cells by β catenin signaling |
| topic | Autophagy Nitric oxide β-catenin Wnt Prostate cancer |
| url | http://www.karger.com/Article/FullText/369749 |
| work_keys_str_mv | AT rongkailin regulationofautophagyofprostatecancercellsbybcateninsignaling AT jiayufeng regulationofautophagyofprostatecancercellsbybcateninsignaling AT shaoliangdong regulationofautophagyofprostatecancercellsbybcateninsignaling AT runyangpan regulationofautophagyofprostatecancercellsbybcateninsignaling AT huiqiangzhuang regulationofautophagyofprostatecancercellsbybcateninsignaling AT zhenyuding regulationofautophagyofprostatecancercellsbybcateninsignaling |