Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum
The study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, heat shock 70 protein 5, adiponectin receptor 1, and adiponectin receptor 2, in the corpus luteum (CL) during luteal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/20/3221 |
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| author | Granit Thaqi Bajram Berisha Michael W. Pfaffl |
| author_facet | Granit Thaqi Bajram Berisha Michael W. Pfaffl |
| author_sort | Granit Thaqi |
| collection | DOAJ |
| description | The study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, heat shock 70 protein 5, adiponectin receptor 1, and adiponectin receptor 2, in the corpus luteum (CL) during luteal regression, also known as luteolysis, in dairy cows. We selected Fleckvieh cows in the mid-luteal phase (days 8–12, control group) and administered cloprostenol (PGF analog) to experimentally induce luteolysis. We collected CL samples at different time points following PGF application: before treatment (days 8–12, control group) and at 0.5, 2, 4, 12, 24, 48, and 64 h post-treatment (n = 5) per group. The mRNA expression was measured via real-time reverse transcription polymerase chain reaction (RT-qPCR). Vaspin was characterized by high mRNA levels at the beginning of the regression stage, followed by a significant decrease 48 h and 64 h after PGF treatment. Adiponectin mRNA levels were elevated 48 h after PGF. Resistin showed upregulation 4 h post PGF application. In summary, the alterations observed in the adipokine family within experimentally induced regressing CL tissue potentially play an integral role in the local regulatory processes governing the sequence of events culminating in functional luteolysis and subsequent structural changes in the bovine ovary. |
| first_indexed | 2024-03-10T21:30:18Z |
| format | Article |
| id | doaj.art-6060a5e93bc44445b6f84bd41d9bf2e0 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T21:30:18Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6060a5e93bc44445b6f84bd41d9bf2e02023-11-19T15:24:50ZengMDPI AGAnimals2076-26152023-10-011320322110.3390/ani13203221Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus LuteumGranit Thaqi0Bajram Berisha1Michael W. Pfaffl2Chair of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephan, 85354 Munich, GermanyChair of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephan, 85354 Munich, GermanyChair of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephan, 85354 Munich, GermanyThe study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, heat shock 70 protein 5, adiponectin receptor 1, and adiponectin receptor 2, in the corpus luteum (CL) during luteal regression, also known as luteolysis, in dairy cows. We selected Fleckvieh cows in the mid-luteal phase (days 8–12, control group) and administered cloprostenol (PGF analog) to experimentally induce luteolysis. We collected CL samples at different time points following PGF application: before treatment (days 8–12, control group) and at 0.5, 2, 4, 12, 24, 48, and 64 h post-treatment (n = 5) per group. The mRNA expression was measured via real-time reverse transcription polymerase chain reaction (RT-qPCR). Vaspin was characterized by high mRNA levels at the beginning of the regression stage, followed by a significant decrease 48 h and 64 h after PGF treatment. Adiponectin mRNA levels were elevated 48 h after PGF. Resistin showed upregulation 4 h post PGF application. In summary, the alterations observed in the adipokine family within experimentally induced regressing CL tissue potentially play an integral role in the local regulatory processes governing the sequence of events culminating in functional luteolysis and subsequent structural changes in the bovine ovary.https://www.mdpi.com/2076-2615/13/20/3221corpus luteumovary functioninduced luteolysisadipokinesgene expressioncattle reproduction |
| spellingShingle | Granit Thaqi Bajram Berisha Michael W. Pfaffl Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum Animals corpus luteum ovary function induced luteolysis adipokines gene expression cattle reproduction |
| title | Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum |
| title_full | Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum |
| title_fullStr | Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum |
| title_full_unstemmed | Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum |
| title_short | Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum |
| title_sort | local expression dynamics of various adipokines during induced luteal regression luteolysis in the bovine corpus luteum |
| topic | corpus luteum ovary function induced luteolysis adipokines gene expression cattle reproduction |
| url | https://www.mdpi.com/2076-2615/13/20/3221 |
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