Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study
Resveratrol (RES) is a bioactive molecule with potent antioxidant properties, and it constitutes an essential component of herbal medicine. This study was designed to use a nano-syntheses approach to encapsulate RES in Poloxamer 407 nanoparticles. This type of nano-construction has been employed in...
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| Format: | Article |
| Language: | English |
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University of Technology, Baghdad
2021-09-01
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| Series: | Journal of Applied Sciences and Nanotechnology |
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| Online Access: | https://jasn.uotechnology.edu.iq/article_15543_d2cade0db40aa60ced6456e9104ad8e1.pdf |
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| author | Ali Al-Jubori Ghassan Sulaiman Amer Tawfeeq |
| author_facet | Ali Al-Jubori Ghassan Sulaiman Amer Tawfeeq |
| author_sort | Ali Al-Jubori |
| collection | DOAJ |
| description | Resveratrol (RES) is a bioactive molecule with potent antioxidant properties, and it constitutes an essential component of herbal medicine. This study was designed to use a nano-syntheses approach to encapsulate RES in Poloxamer 407 nanoparticles. This type of nano-construction has been employed in a variety of biological applications, both in vitro and in vivo. The contribution of this nano-construct is to increase antioxidant activity over the pure RES was investigate. 1,1-diphenyl-2-picryl hydrazyl (DPPH), and hydrogen peroxide radical scavenging assays were utilized in vitro. RES-loaded poloxamer 407 showed concentration-dependent scavenging action in the range of 20-80 µg.ml<sup>-1</sup>, with a maximum activity of 80.1% at 80 µg.ml<sup>-1</sup>. Whereas pure RES had a decrement of 61.7%. While the activity of positive control (Vit. C) was 93.2%. The magnitude of blood hemolysis examination was 3.9% at 80 µg.ml<sup>-1</sup>. That may indicate RES-loaded poloxamer 407 provide significant red blood cell protection. The pure RES did not prevent the appearance of abnormal cells (echinocytes), and that was cured by RES-loaded poloxamer 407. Finally, the safety of RES-loaded poloxamer 407 was assessed in vivo. Male mice were invested to detect the functions of their liver and kidney. A histopathological study was included as well. The findings showed that RES-loaded poloxamer 407 might have superior characters as a drug delivery system, nutritional supplements, and may be used in pharmaceutical products. |
| first_indexed | 2024-12-22T02:03:07Z |
| format | Article |
| id | doaj.art-60614081b9b444ea8f26c60d0780834b |
| institution | Directory Open Access Journal |
| issn | 2788-6867 |
| language | English |
| last_indexed | 2024-12-22T02:03:07Z |
| publishDate | 2021-09-01 |
| publisher | University of Technology, Baghdad |
| record_format | Article |
| series | Journal of Applied Sciences and Nanotechnology |
| spelling | doaj.art-60614081b9b444ea8f26c60d0780834b2022-12-21T18:42:36ZengUniversity of Technology, BaghdadJournal of Applied Sciences and Nanotechnology2788-68672021-09-011311210.53293/jasn.2021.3809.104615543Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo StudyAli Al-Jubori0Ghassan Sulaiman1Amer Tawfeeq2Division of Biotechnology, Department of Applied Sciences, University of Technology, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, IraqDepartment of Molecular Biology, Iraqi Center for Cancer and Medical Genetics Research, Mustansiriyah University, Iraq.Resveratrol (RES) is a bioactive molecule with potent antioxidant properties, and it constitutes an essential component of herbal medicine. This study was designed to use a nano-syntheses approach to encapsulate RES in Poloxamer 407 nanoparticles. This type of nano-construction has been employed in a variety of biological applications, both in vitro and in vivo. The contribution of this nano-construct is to increase antioxidant activity over the pure RES was investigate. 1,1-diphenyl-2-picryl hydrazyl (DPPH), and hydrogen peroxide radical scavenging assays were utilized in vitro. RES-loaded poloxamer 407 showed concentration-dependent scavenging action in the range of 20-80 µg.ml<sup>-1</sup>, with a maximum activity of 80.1% at 80 µg.ml<sup>-1</sup>. Whereas pure RES had a decrement of 61.7%. While the activity of positive control (Vit. C) was 93.2%. The magnitude of blood hemolysis examination was 3.9% at 80 µg.ml<sup>-1</sup>. That may indicate RES-loaded poloxamer 407 provide significant red blood cell protection. The pure RES did not prevent the appearance of abnormal cells (echinocytes), and that was cured by RES-loaded poloxamer 407. Finally, the safety of RES-loaded poloxamer 407 was assessed in vivo. Male mice were invested to detect the functions of their liver and kidney. A histopathological study was included as well. The findings showed that RES-loaded poloxamer 407 might have superior characters as a drug delivery system, nutritional supplements, and may be used in pharmaceutical products.https://jasn.uotechnology.edu.iq/article_15543_d2cade0db40aa60ced6456e9104ad8e1.pdfresveratrolpoloxamer 407dpphin vivoanimal model |
| spellingShingle | Ali Al-Jubori Ghassan Sulaiman Amer Tawfeeq Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study Journal of Applied Sciences and Nanotechnology resveratrol poloxamer 407 dpph in vivo animal model |
| title | Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study |
| title_full | Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study |
| title_fullStr | Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study |
| title_full_unstemmed | Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study |
| title_short | Antioxidant Activities of Resveratrol Loaded Poloxamer 407: An In Vitro and In Vivo Study |
| title_sort | antioxidant activities of resveratrol loaded poloxamer 407 an in vitro and in vivo study |
| topic | resveratrol poloxamer 407 dpph in vivo animal model |
| url | https://jasn.uotechnology.edu.iq/article_15543_d2cade0db40aa60ced6456e9104ad8e1.pdf |
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