Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME)
Most of <i>R</i> (resistance) genes encode the protein containing NBS-LRR (nucleotide binding site and leucine-rich repeat) domains. Here, <i>N. benthamiana</i> plants were used for transient expression assays at 3–4 weeks of age. We identified a TNL (TIR-NBS-LRR) encoding ge...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/10/2032 |
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| author | Pengfei Diao Hongyu Sun Zhuo Bao Wenxia Li Niu Niu Weimin Li Hada Wuriyanghan |
| author_facet | Pengfei Diao Hongyu Sun Zhuo Bao Wenxia Li Niu Niu Weimin Li Hada Wuriyanghan |
| author_sort | Pengfei Diao |
| collection | DOAJ |
| description | Most of <i>R</i> (resistance) genes encode the protein containing NBS-LRR (nucleotide binding site and leucine-rich repeat) domains. Here, <i>N. benthamiana</i> plants were used for transient expression assays at 3–4 weeks of age. We identified a TNL (TIR-NBS-LRR) encoding gene <i>GmRUN1</i> that was resistant to both soybean mosaic virus (SMV) and tobacco mosaic virus (TMV). Truncation analysis indicated the importance of all three canonical domains for GmRUN1-mediated antiviral activity. Promoter-GUS analysis showed that <i>GmRUN1</i> expression is inducible by both salicylic acid (SA) and a transcription factor <i>GmDREB3</i> via the cis-elements as-1 and ERE (ethylene response element), which are present in its promoter region. Interestingly, <i>GmRUN1</i> gDNA (genomic DNA) shows higher viral resistance than its cDNA (complementary DNA), indicating the existence of intron-mediated enhancement (IME) for <i>GmRUN1</i> regulation. We provided evidence that intron2 of <i>GmRUN1</i> increased the mRNA level of native gene <i>GmRUN1</i>, a soybean antiviral gene <i>SRC7</i> and also a reporter gene Luciferase, indicating the general transcriptional enhancement of intron2 in different genes. In summary, we identified an antiviral <i>TNL</i> type soybean gene <i>GmRUN1</i>, expression of which was regulated at different layers. The investigation of <i>GmRUN1</i> gene regulatory network would help to explore the mechanism underlying soybean-SMV interactions. |
| first_indexed | 2024-03-10T06:08:28Z |
| format | Article |
| id | doaj.art-60654f4c4d924b7daa5ce2dba8cad31d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T06:08:28Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-60654f4c4d924b7daa5ce2dba8cad31d2023-11-22T20:19:45ZengMDPI AGViruses1999-49152021-10-011310203210.3390/v13102032Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME)Pengfei Diao0Hongyu Sun1Zhuo Bao2Wenxia Li3Niu Niu4Weimin Li5Hada Wuriyanghan6Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaMost of <i>R</i> (resistance) genes encode the protein containing NBS-LRR (nucleotide binding site and leucine-rich repeat) domains. Here, <i>N. benthamiana</i> plants were used for transient expression assays at 3–4 weeks of age. We identified a TNL (TIR-NBS-LRR) encoding gene <i>GmRUN1</i> that was resistant to both soybean mosaic virus (SMV) and tobacco mosaic virus (TMV). Truncation analysis indicated the importance of all three canonical domains for GmRUN1-mediated antiviral activity. Promoter-GUS analysis showed that <i>GmRUN1</i> expression is inducible by both salicylic acid (SA) and a transcription factor <i>GmDREB3</i> via the cis-elements as-1 and ERE (ethylene response element), which are present in its promoter region. Interestingly, <i>GmRUN1</i> gDNA (genomic DNA) shows higher viral resistance than its cDNA (complementary DNA), indicating the existence of intron-mediated enhancement (IME) for <i>GmRUN1</i> regulation. We provided evidence that intron2 of <i>GmRUN1</i> increased the mRNA level of native gene <i>GmRUN1</i>, a soybean antiviral gene <i>SRC7</i> and also a reporter gene Luciferase, indicating the general transcriptional enhancement of intron2 in different genes. In summary, we identified an antiviral <i>TNL</i> type soybean gene <i>GmRUN1</i>, expression of which was regulated at different layers. The investigation of <i>GmRUN1</i> gene regulatory network would help to explore the mechanism underlying soybean-SMV interactions.https://www.mdpi.com/1999-4915/13/10/2032<i>GmDREB3</i><i>GmRUN1</i>intron-mediated enhancementresistance genesalicylic acidsoybean mosaic virus |
| spellingShingle | Pengfei Diao Hongyu Sun Zhuo Bao Wenxia Li Niu Niu Weimin Li Hada Wuriyanghan Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) Viruses <i>GmDREB3</i> <i>GmRUN1</i> intron-mediated enhancement resistance gene salicylic acid soybean mosaic virus |
| title | Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) |
| title_full | Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) |
| title_fullStr | Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) |
| title_full_unstemmed | Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) |
| title_short | Expression of an Antiviral Gene <i>GmRUN1</i> from Soybean Is Regulated via Intron-Mediated Enhancement (IME) |
| title_sort | expression of an antiviral gene i gmrun1 i from soybean is regulated via intron mediated enhancement ime |
| topic | <i>GmDREB3</i> <i>GmRUN1</i> intron-mediated enhancement resistance gene salicylic acid soybean mosaic virus |
| url | https://www.mdpi.com/1999-4915/13/10/2032 |
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