Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease
Periodontitis is associated with elevated C-reactive protein (CRP) in both serum and gingival crevicular fluid (GCF). Although the liver is the primary source of CRP, extra-hepatic production of CRP has been reported. This study aimed to determine whether CRP in GCF is produced locally in the gingiv...
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| Format: | Article |
| Language: | English |
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Romanian Society of Oral Rehabilitation
2012-07-01
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| Series: | Romanian Journal of Oral Rehabilitation |
| Subjects: | |
| Online Access: | http://www.rjor.ro/identification-of-c-reactive-protein-from-gingival-crevicular-fluid-in-systemic-disease/?lang=ro |
| _version_ | 1817998543349088256 |
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| author | Amelia Surdu Macovei Liliana Pasarin Oana Potarnichie Silvia Martu |
| author_facet | Amelia Surdu Macovei Liliana Pasarin Oana Potarnichie Silvia Martu |
| author_sort | Amelia Surdu Macovei |
| collection | DOAJ |
| description | Periodontitis is associated with elevated C-reactive protein (CRP) in both serum and gingival crevicular fluid (GCF). Although the liver is the primary source of CRP, extra-hepatic production of CRP has been reported. This study aimed to determine whether CRP in GCF is produced locally in the gingivae. Material and methods Gingivae and GCF were collected from non-periodontitis and periodontitis sites. Presence of CRP in gingivae was assessed by immunohistochemistry. CRP in GCF was measured using ELISA. Gene expression for CRP in gingivae was determined using real-time polymerase chain reaction. Results CRP was found in both the gingivae and GCF. No gingivae had detectable amounts of CRP mRNA. Not all patients with periodontitis had detectable levels of CRP in the GCF. Some non-periodontitis patients had detectable levels of CRP in the GCF. Conclusions CRP in the GCF appears to be of systemic origin, and therefore may be indicative of systemic inflammation from either a periodontal infection or inflammatory disease elsewhere. The correlation between levels of CRP in GCF and serum requires validation in future studies. |
| first_indexed | 2024-04-14T02:54:43Z |
| format | Article |
| id | doaj.art-606753d9d7df4a0cb0150faa48fb0f26 |
| institution | Directory Open Access Journal |
| issn | 2066-7000 2601-4661 |
| language | English |
| last_indexed | 2024-04-14T02:54:43Z |
| publishDate | 2012-07-01 |
| publisher | Romanian Society of Oral Rehabilitation |
| record_format | Article |
| series | Romanian Journal of Oral Rehabilitation |
| spelling | doaj.art-606753d9d7df4a0cb0150faa48fb0f262022-12-22T02:16:08ZengRomanian Society of Oral RehabilitationRomanian Journal of Oral Rehabilitation2066-70002601-46612012-07-01426771Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic DiseaseAmelia Surdu Macovei0Liliana Pasarin1Oana Potarnichie2Silvia Martu3Department of Periodontology, Faculty of Dental Medicine University of Medicine and Pharmacy "Grigore T. Popa" - Iasi, RomaniaDepartment of Periodontology, Faculty of Dental Medicine University of Medicine and Pharmacy "Grigore T. Popa" - Iasi, RomaniaDepartment of Periodontology, Faculty of Dental Medicine University of Medicine and Pharmacy "Grigore T. Popa" - Iasi, RomaniaDepartment of Periodontology, Faculty of Dental Medicine University of Medicine and Pharmacy "Grigore T. Popa" - Iasi, RomaniaPeriodontitis is associated with elevated C-reactive protein (CRP) in both serum and gingival crevicular fluid (GCF). Although the liver is the primary source of CRP, extra-hepatic production of CRP has been reported. This study aimed to determine whether CRP in GCF is produced locally in the gingivae. Material and methods Gingivae and GCF were collected from non-periodontitis and periodontitis sites. Presence of CRP in gingivae was assessed by immunohistochemistry. CRP in GCF was measured using ELISA. Gene expression for CRP in gingivae was determined using real-time polymerase chain reaction. Results CRP was found in both the gingivae and GCF. No gingivae had detectable amounts of CRP mRNA. Not all patients with periodontitis had detectable levels of CRP in the GCF. Some non-periodontitis patients had detectable levels of CRP in the GCF. Conclusions CRP in the GCF appears to be of systemic origin, and therefore may be indicative of systemic inflammation from either a periodontal infection or inflammatory disease elsewhere. The correlation between levels of CRP in GCF and serum requires validation in future studies.http://www.rjor.ro/identification-of-c-reactive-protein-from-gingival-crevicular-fluid-in-systemic-disease/?lang=roC-reactive protein; inflammation; gingival crevicular fluid |
| spellingShingle | Amelia Surdu Macovei Liliana Pasarin Oana Potarnichie Silvia Martu Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease Romanian Journal of Oral Rehabilitation C-reactive protein; inflammation; gingival crevicular fluid |
| title | Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease |
| title_full | Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease |
| title_fullStr | Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease |
| title_full_unstemmed | Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease |
| title_short | Identification of C-Reactive Protein from Gingival Crevicular Fluid in Systemic Disease |
| title_sort | identification of c reactive protein from gingival crevicular fluid in systemic disease |
| topic | C-reactive protein; inflammation; gingival crevicular fluid |
| url | http://www.rjor.ro/identification-of-c-reactive-protein-from-gingival-crevicular-fluid-in-systemic-disease/?lang=ro |
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