Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development
Intrathymic differentiation of T lymphocytes begins as early as intrauterine stage, yet the T cell lineage decisions of human fetal thymocytes at different gestational ages are not currently understood. Here, we performed integrative single-cell analyses of thymocytes across gestational ages. We ide...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.679616/full |
| _version_ | 1819148905018818560 |
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| author | Yuchen Li Yuchen Li Yuchen Li Weihong Zeng Weihong Zeng Weihong Zeng Tong Li Tong Li Tong Li Yanyan Guo Yanyan Guo Yanyan Guo Guangyong Zheng Xiaoying He Xiaoying He Lilian Bai Lilian Bai Lilian Bai Guolian Ding Guolian Ding Guolian Ding Li Jin Li Jin Xinmei Liu Xinmei Liu Xinmei Liu |
| author_facet | Yuchen Li Yuchen Li Yuchen Li Weihong Zeng Weihong Zeng Weihong Zeng Tong Li Tong Li Tong Li Yanyan Guo Yanyan Guo Yanyan Guo Guangyong Zheng Xiaoying He Xiaoying He Lilian Bai Lilian Bai Lilian Bai Guolian Ding Guolian Ding Guolian Ding Li Jin Li Jin Xinmei Liu Xinmei Liu Xinmei Liu |
| author_sort | Yuchen Li |
| collection | DOAJ |
| description | Intrathymic differentiation of T lymphocytes begins as early as intrauterine stage, yet the T cell lineage decisions of human fetal thymocytes at different gestational ages are not currently understood. Here, we performed integrative single-cell analyses of thymocytes across gestational ages. We identified conserved candidates underlying the selection of T cell receptor (TCR) lineages in different human fetal stages. The trajectory of early thymocyte commitment during fetal growth was also characterized. Comparisons with mouse data revealed conserved and species-specific transcriptional dynamics of thymocyte proliferation, apoptosis and selection. Genome-wide association study (GWAS) data associated with multiple autoimmune disorders were analyzed to characterize susceptibility genes that are highly expressed at specific stages during fetal thymocyte development. In summary, our integrative map describes previously underappreciated aspects of human thymocyte development, and provides a comprehensive reference for understanding T cell lymphopoiesis in a self-tolerant and functional adaptive immune system. |
| first_indexed | 2024-12-22T13:53:08Z |
| format | Article |
| id | doaj.art-606aae261f194f01a2442427e33713fc |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-22T13:53:08Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-606aae261f194f01a2442427e33713fc2022-12-21T18:23:37ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-07-011210.3389/fgene.2021.679616679616Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte DevelopmentYuchen Li0Yuchen Li1Yuchen Li2Weihong Zeng3Weihong Zeng4Weihong Zeng5Tong Li6Tong Li7Tong Li8Yanyan Guo9Yanyan Guo10Yanyan Guo11Guangyong Zheng12Xiaoying He13Xiaoying He14Lilian Bai15Lilian Bai16Lilian Bai17Guolian Ding18Guolian Ding19Guolian Ding20Li Jin21Li Jin22Xinmei Liu23Xinmei Liu24Xinmei Liu25International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaBio-Med Big Data Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaShanghai Key Laboratory of Embryo Original Disease, Shanghai, ChinaResearch Units of Embryo Original Diseases, Chinese Academy of Medical Sciences, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaIntrathymic differentiation of T lymphocytes begins as early as intrauterine stage, yet the T cell lineage decisions of human fetal thymocytes at different gestational ages are not currently understood. Here, we performed integrative single-cell analyses of thymocytes across gestational ages. We identified conserved candidates underlying the selection of T cell receptor (TCR) lineages in different human fetal stages. The trajectory of early thymocyte commitment during fetal growth was also characterized. Comparisons with mouse data revealed conserved and species-specific transcriptional dynamics of thymocyte proliferation, apoptosis and selection. Genome-wide association study (GWAS) data associated with multiple autoimmune disorders were analyzed to characterize susceptibility genes that are highly expressed at specific stages during fetal thymocyte development. In summary, our integrative map describes previously underappreciated aspects of human thymocyte development, and provides a comprehensive reference for understanding T cell lymphopoiesis in a self-tolerant and functional adaptive immune system.https://www.frontiersin.org/articles/10.3389/fgene.2021.679616/fullfetal thymushuman and murinesingle-cell RNA-seqT lymphopoiesistranscriptional dynamics |
| spellingShingle | Yuchen Li Yuchen Li Yuchen Li Weihong Zeng Weihong Zeng Weihong Zeng Tong Li Tong Li Tong Li Yanyan Guo Yanyan Guo Yanyan Guo Guangyong Zheng Xiaoying He Xiaoying He Lilian Bai Lilian Bai Lilian Bai Guolian Ding Guolian Ding Guolian Ding Li Jin Li Jin Xinmei Liu Xinmei Liu Xinmei Liu Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development Frontiers in Genetics fetal thymus human and murine single-cell RNA-seq T lymphopoiesis transcriptional dynamics |
| title | Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development |
| title_full | Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development |
| title_fullStr | Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development |
| title_full_unstemmed | Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development |
| title_short | Integrative Single-Cell Transcriptomic Analysis of Human Fetal Thymocyte Development |
| title_sort | integrative single cell transcriptomic analysis of human fetal thymocyte development |
| topic | fetal thymus human and murine single-cell RNA-seq T lymphopoiesis transcriptional dynamics |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.679616/full |
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