Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis
Objective Explore the role and status of tumor necrosis factor receptor⁃associated factor 6 (TRAF6) in the inflammatory response of human osteoblast⁃like cells MG63 which was triggered by Enterococcus faecalis (E. faeca⁃ lis) and its lipoteichoic acid (LTA). Methods SiRNA technology was applied to...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2017-07-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.07.003 |
| _version_ | 1818036285372104704 |
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| author | LU Yu LIU Chengxia LIU Zhongjun |
| author_facet | LU Yu LIU Chengxia LIU Zhongjun |
| author_sort | LU Yu |
| collection | DOAJ |
| description | Objective Explore the role and status of tumor necrosis factor receptor⁃associated factor 6 (TRAF6) in
the inflammatory response of human osteoblast⁃like cells MG63 which was triggered by Enterococcus faecalis (E. faeca⁃
lis) and its lipoteichoic acid (LTA). Methods SiRNA technology was applied to silence the TRAF6 gene of MG63
cells, Using E.faecelis and its LTA to stimulate the silence MG63 cells with different hours. After that, using real⁃time
PCR technology to detect toll⁃like receptor 2 (TLR2) and TRAF6 gene expression and using ELISA assay to detect pro⁃
inflammatory cytokines interleukin⁃1β and TNF⁃alpha expression levels. Results When MG63 cells was infected by
E. faecalis, its LTA, TLR2 and TRAF6 gene level has increased to varying degrees (P < 0.05); interleukin⁃1β and TNF⁃
alpha expression was significantly higher (P < 0.05). When TRAF6 gene of MG63 cells was silenced by siRNA, pro⁃in⁃
flammatory cytokines interleukin ⁃ 1β, interleukin ⁃6, interleukin ⁃8 and TNF ⁃alpha expression decreased significantly
(P < 0.05). Conclusion E. faecalis and its toxic components is identified by MG63 cells mainly through TLR2 recep⁃
tors. The major virulence factor in periapical infections caused by E. faecalis is LTA. |
| first_indexed | 2024-12-10T07:08:31Z |
| format | Article |
| id | doaj.art-606c692c0e26448ea9ccaf0bd772a67f |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-12-10T07:08:31Z |
| publishDate | 2017-07-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-606c692c0e26448ea9ccaf0bd772a67f2022-12-22T01:58:07ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562017-07-0125742042510.12016/j.issn.2096⁃1456.2017.07.003Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalisLU Yu0LIU Chengxia1 LIU Zhongjun2 Department of Endodontics, Stomatological Hospital, Southern Medical University Department of Endodontics, Stomatological Hospital, Southern Medical University Department of Endodontics, Stomatological Hospital, Southern Medical UniversityObjective Explore the role and status of tumor necrosis factor receptor⁃associated factor 6 (TRAF6) in the inflammatory response of human osteoblast⁃like cells MG63 which was triggered by Enterococcus faecalis (E. faeca⁃ lis) and its lipoteichoic acid (LTA). Methods SiRNA technology was applied to silence the TRAF6 gene of MG63 cells, Using E.faecelis and its LTA to stimulate the silence MG63 cells with different hours. After that, using real⁃time PCR technology to detect toll⁃like receptor 2 (TLR2) and TRAF6 gene expression and using ELISA assay to detect pro⁃ inflammatory cytokines interleukin⁃1β and TNF⁃alpha expression levels. Results When MG63 cells was infected by E. faecalis, its LTA, TLR2 and TRAF6 gene level has increased to varying degrees (P < 0.05); interleukin⁃1β and TNF⁃ alpha expression was significantly higher (P < 0.05). When TRAF6 gene of MG63 cells was silenced by siRNA, pro⁃in⁃ flammatory cytokines interleukin ⁃ 1β, interleukin ⁃6, interleukin ⁃8 and TNF ⁃alpha expression decreased significantly (P < 0.05). Conclusion E. faecalis and its toxic components is identified by MG63 cells mainly through TLR2 recep⁃ tors. The major virulence factor in periapical infections caused by E. faecalis is LTA.http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.07.003E. faecalisLTATLR2Human osteoblast⁃like cellsTRAF6 |
| spellingShingle | LU Yu LIU Chengxia LIU Zhongjun Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis 口腔疾病防治 E. faecalis LTA TLR2 Human osteoblast⁃like cells TRAF6 |
| title | Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis |
| title_full | Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis |
| title_fullStr | Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis |
| title_full_unstemmed | Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis |
| title_short | Role of TRAF6 in inflammatory responses of human osteoblast ⁃like cells with Enterococcus faecalis |
| title_sort | role of traf6 in inflammatory responses of human osteoblast ⁃like cells with enterococcus faecalis |
| topic | E. faecalis LTA TLR2 Human osteoblast⁃like cells TRAF6 |
| url | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2017.07.003 |
| work_keys_str_mv | AT luyu roleoftraf6ininflammatoryresponsesofhumanosteoblastlikecellswithenterococcusfaecalis AT liuchengxia roleoftraf6ininflammatoryresponsesofhumanosteoblastlikecellswithenterococcusfaecalis AT liuzhongjun roleoftraf6ininflammatoryresponsesofhumanosteoblastlikecellswithenterococcusfaecalis |