In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC)
Enterotoxigenic <i>Escherichia coli</i> (ETEC) causing post-weaning diarrhea (PWD) in piglets have a detrimental impact on animal health and economy in pig production. ETEC strains can adhere to the host’s small intestinal epithelial cells using fimbriae such as F4 and F18. Phage therapy...
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MDPI AG
2023-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/5/1053 |
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| author | Margaux Navez Céline Antoine Fanny Laforêt Elizabeth Goya-Jorge Caroline Douny Marie-Louise Scippo Marjorie Vermeersch Jean-Noël Duprez Georges Daube Jacques Mainil Bernard Taminiau Véronique Delcenserie Damien Thiry |
| author_facet | Margaux Navez Céline Antoine Fanny Laforêt Elizabeth Goya-Jorge Caroline Douny Marie-Louise Scippo Marjorie Vermeersch Jean-Noël Duprez Georges Daube Jacques Mainil Bernard Taminiau Véronique Delcenserie Damien Thiry |
| author_sort | Margaux Navez |
| collection | DOAJ |
| description | Enterotoxigenic <i>Escherichia coli</i> (ETEC) causing post-weaning diarrhea (PWD) in piglets have a detrimental impact on animal health and economy in pig production. ETEC strains can adhere to the host’s small intestinal epithelial cells using fimbriae such as F4 and F18. Phage therapy could represent an interesting alternative to antimicrobial resistance against ETEC infections. In this study, four bacteriophages, named vB_EcoS_ULIM2, vB_EcoM_ULIM3, vB_EcoM_ULIM8 and vB_EcoM_ULIM9, were isolated against an O8:F18 <i>E. coli</i> strain (A-I-210) and selected based on their host range. These phages were characterized in vitro, showing a lytic activity over a pH (4–10) and temperature (25–45 °C) range. According to genomic analysis, these bacteriophages belong to the <i>Caudoviricetes</i> class. No gene related to lysogeny was identified. The in vivo <i>Galleria mellonella</i> larvae model suggested the therapeutic potential of one selected phage, vB_EcoS_ULIM2, with a statistically significant increase in survival compared to non-treated larvae. To assess the effect of this phage on the piglet gut microbiota, vB_EcoS_ULIM2 was inoculated in a static model simulating the piglet intestinal microbial ecosystem for 72 h. This study shows that this phage replicates efficiently both in vitro and in vivo in a <i>Galleria mellonella</i> model and reveals the safety of the phage-based treatment on the piglet microbiota. |
| first_indexed | 2024-03-11T03:14:22Z |
| format | Article |
| id | doaj.art-607002d6c1f242bd92165eb8100800ae |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T03:14:22Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-607002d6c1f242bd92165eb8100800ae2023-11-18T03:38:15ZengMDPI AGViruses1999-49152023-04-01155105310.3390/v15051053In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC)Margaux Navez0Céline Antoine1Fanny Laforêt2Elizabeth Goya-Jorge3Caroline Douny4Marie-Louise Scippo5Marjorie Vermeersch6Jean-Noël Duprez7Georges Daube8Jacques Mainil9Bernard Taminiau10Véronique Delcenserie11Damien Thiry12Laboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Food Quality Management, Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liege, BelgiumLaboratory of Food Analysis, Department of Food Sciences, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liege, BelgiumLaboratory of Food Analysis, Department of Food Sciences, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liege, BelgiumCenter for Microscopy and Molecular Imaging, Electron Microscopy Laboratory, ULB, 6041 Gosselies, BelgiumLaboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Food Microbiology, Fundamental and Applied Research for Animals & Health (FARAH), Department of Food Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Food Microbiology, Fundamental and Applied Research for Animals & Health (FARAH), Department of Food Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumLaboratory of Food Quality Management, Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liege, BelgiumLaboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liege, 4000 Liege, BelgiumEnterotoxigenic <i>Escherichia coli</i> (ETEC) causing post-weaning diarrhea (PWD) in piglets have a detrimental impact on animal health and economy in pig production. ETEC strains can adhere to the host’s small intestinal epithelial cells using fimbriae such as F4 and F18. Phage therapy could represent an interesting alternative to antimicrobial resistance against ETEC infections. In this study, four bacteriophages, named vB_EcoS_ULIM2, vB_EcoM_ULIM3, vB_EcoM_ULIM8 and vB_EcoM_ULIM9, were isolated against an O8:F18 <i>E. coli</i> strain (A-I-210) and selected based on their host range. These phages were characterized in vitro, showing a lytic activity over a pH (4–10) and temperature (25–45 °C) range. According to genomic analysis, these bacteriophages belong to the <i>Caudoviricetes</i> class. No gene related to lysogeny was identified. The in vivo <i>Galleria mellonella</i> larvae model suggested the therapeutic potential of one selected phage, vB_EcoS_ULIM2, with a statistically significant increase in survival compared to non-treated larvae. To assess the effect of this phage on the piglet gut microbiota, vB_EcoS_ULIM2 was inoculated in a static model simulating the piglet intestinal microbial ecosystem for 72 h. This study shows that this phage replicates efficiently both in vitro and in vivo in a <i>Galleria mellonella</i> model and reveals the safety of the phage-based treatment on the piglet microbiota.https://www.mdpi.com/1999-4915/15/5/1053bacteriophagesphage therapyETEC<i>Galleria mellonella</i>weaning pigletsmicrobiota |
| spellingShingle | Margaux Navez Céline Antoine Fanny Laforêt Elizabeth Goya-Jorge Caroline Douny Marie-Louise Scippo Marjorie Vermeersch Jean-Noël Duprez Georges Daube Jacques Mainil Bernard Taminiau Véronique Delcenserie Damien Thiry In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) Viruses bacteriophages phage therapy ETEC <i>Galleria mellonella</i> weaning piglets microbiota |
| title | In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) |
| title_full | In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) |
| title_fullStr | In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) |
| title_full_unstemmed | In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) |
| title_short | In Vitro Effect on Piglet Gut Microbiota and In Vivo Assessment of Newly Isolated Bacteriophages against F18 Enterotoxigenic <i>Escherichia coli</i> (ETEC) |
| title_sort | in vitro effect on piglet gut microbiota and in vivo assessment of newly isolated bacteriophages against f18 enterotoxigenic i escherichia coli i etec |
| topic | bacteriophages phage therapy ETEC <i>Galleria mellonella</i> weaning piglets microbiota |
| url | https://www.mdpi.com/1999-4915/15/5/1053 |
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