Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers
Crystalline amino acids (AAs) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to inve...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120306520 |
| _version_ | 1818429447068450816 |
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| author | Mingfa Sun Hongchao Jiao Xiaojuan Wang Victoria A. Uyanga Jingpeng Zhao Hai Lin |
| author_facet | Mingfa Sun Hongchao Jiao Xiaojuan Wang Victoria A. Uyanga Jingpeng Zhao Hai Lin |
| author_sort | Mingfa Sun |
| collection | DOAJ |
| description | Crystalline amino acids (AAs) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl (Lys) and DL-methionine (DL-Met) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets (80CLM, 60CLM), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group (P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group (P < 0.05), but increased villi height and B0AT mRNA expression level (P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group (P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens. |
| first_indexed | 2024-12-14T15:17:39Z |
| format | Article |
| id | doaj.art-6070096919c743ebbe1444334bad8b85 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-14T15:17:39Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-6070096919c743ebbe1444334bad8b852022-12-21T22:56:15ZengElsevierPoultry Science0032-57912020-12-01991269146924Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilersMingfa Sun0Hongchao Jiao1Xiaojuan Wang2Victoria A. Uyanga3Jingpeng Zhao4Hai Lin5Department of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaDepartment of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaDepartment of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaDepartment of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaCorresponding authors:; Department of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaCorresponding authors:; Department of Animal Science, Shandong Agricultural University, Shandong Key Lab for Animal Biotechnology and Disease Control and Prevention, Taian City, Shandong Province, 271018, ChinaCrystalline amino acids (AAs) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl (Lys) and DL-methionine (DL-Met) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets (80CLM, 60CLM), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group (P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group (P < 0.05), but increased villi height and B0AT mRNA expression level (P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group (P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens.http://www.sciencedirect.com/science/article/pii/S0032579120306520broilerencapsulated amino acidlysineDL-methionine |
| spellingShingle | Mingfa Sun Hongchao Jiao Xiaojuan Wang Victoria A. Uyanga Jingpeng Zhao Hai Lin Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers Poultry Science broiler encapsulated amino acid lysine DL-methionine |
| title | Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers |
| title_full | Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers |
| title_fullStr | Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers |
| title_full_unstemmed | Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers |
| title_short | Encapsulated crystalline lysine and DL-methionine have higher efficiency than the crystalline form in broilers |
| title_sort | encapsulated crystalline lysine and dl methionine have higher efficiency than the crystalline form in broilers |
| topic | broiler encapsulated amino acid lysine DL-methionine |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120306520 |
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