Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo
Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was perfo...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2015-03-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | http://www.e-jarb.org/journal/view.html?uid=1460&vmd=Full |
| _version_ | 1818645277154738176 |
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| author | Chang-Woon Kim Sunkyu Han Changyong Choe |
| author_facet | Chang-Woon Kim Sunkyu Han Changyong Choe |
| author_sort | Chang-Woon Kim |
| collection | DOAJ |
| description | Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen (10 μg/ml) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary. |
| first_indexed | 2024-12-17T00:28:11Z |
| format | Article |
| id | doaj.art-607667e41a124740afc138dfb79948a7 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-17T00:28:11Z |
| publishDate | 2015-03-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-607667e41a124740afc138dfb79948a72022-12-21T22:10:24ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-03-01301172110.12750/JET.2015.30.1.17Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of HanwooChang-Woon Kim0Sunkyu Han1Changyong Choe2Department of Obstetrics and Gynecology, Samsung Changwon Hospital,Sungkyunkwan University School of Medicine, Changwon 630-522, South KoreaDepartment of Molecular Biology, Pusan National University, Busan 609-735, South KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, South KoreaAlteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen (10 μg/ml) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary.http://www.e-jarb.org/journal/view.html?uid=1460&vmd=Fullaquaporinkorean cattlefollicular cystestrogen |
| spellingShingle | Chang-Woon Kim Sunkyu Han Changyong Choe Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo Journal of Animal Reproduction and Biotechnology aquaporin korean cattle follicular cyst estrogen |
| title | Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo |
| title_full | Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo |
| title_fullStr | Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo |
| title_full_unstemmed | Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo |
| title_short | Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo |
| title_sort | patterns of aquaporin 7 expression in normal follicles and follicular cyst follicles of hanwoo |
| topic | aquaporin korean cattle follicular cyst estrogen |
| url | http://www.e-jarb.org/journal/view.html?uid=1460&vmd=Full |
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