Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics
Background: Uncoupling protein 1 (UCP1) and UCP2 are associated with tumor metabolism and immunity. However, the prognostic value and molecular mechanisms underlying their action in breast cancer (BC) remain unclear.Materials and methods: In TCGA-BRCA cohort, we investigated the expression character...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.891731/full |
| _version_ | 1818469459276333056 |
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| author | Xin Yu Manman Shi Qi Wu Qi Wu Wen Wei Shengrong Sun Shan Zhu |
| author_facet | Xin Yu Manman Shi Qi Wu Qi Wu Wen Wei Shengrong Sun Shan Zhu |
| author_sort | Xin Yu |
| collection | DOAJ |
| description | Background: Uncoupling protein 1 (UCP1) and UCP2 are associated with tumor metabolism and immunity. However, the prognostic value and molecular mechanisms underlying their action in breast cancer (BC) remain unclear.Materials and methods: In TCGA-BRCA cohort, we investigated the expression characteristics of UCP mRNAs, analyzed their prognostic value by Kaplan-Meier survival analysis, their potential molecular functions by gene set enrichment analysis, and their relationship with immune infiltrating cell types using TIMER and CIBERSORT, along with the assessment of their association with mutational profiles. Kaplan-Meier survival analysis was performed for UCPs in our cohort and their association with BC thermogenesis was assessed by thermal tomography.Results: High expression of UCP1 and UCP2 were positive prognostic markers for BC. UCP1 was associated with the impaired glucose metabolism, while UCP2 with enhanced anti-tumor immunity. High expressions of UCP1 and UCP2 were associated with CDH1 mutations. High UCP1 expression was associated with a high rate of thermogenesis in BC.Conclusions: These results implied a key role of UCP1 and UCP2 in prognosis, metabolism, and immune infiltration in BC. Further investigation of the relevant molecular mechanisms may provide new strategies for BC treatment. |
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| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T21:24:03Z |
| publishDate | 2022-07-01 |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-6077d6b287ff4fc09f12f3e24c52772b2022-12-22T02:29:22ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-07-011010.3389/fcell.2022.891731891731Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and BioinformaticsXin Yu0Manman Shi1Qi Wu2Qi Wu3Wen Wei4Shengrong Sun5Shan Zhu6Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaTongji University Cancer Center, Tenth People’s Hospital of Tongji University, Shanghai, ChinaDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaBackground: Uncoupling protein 1 (UCP1) and UCP2 are associated with tumor metabolism and immunity. However, the prognostic value and molecular mechanisms underlying their action in breast cancer (BC) remain unclear.Materials and methods: In TCGA-BRCA cohort, we investigated the expression characteristics of UCP mRNAs, analyzed their prognostic value by Kaplan-Meier survival analysis, their potential molecular functions by gene set enrichment analysis, and their relationship with immune infiltrating cell types using TIMER and CIBERSORT, along with the assessment of their association with mutational profiles. Kaplan-Meier survival analysis was performed for UCPs in our cohort and their association with BC thermogenesis was assessed by thermal tomography.Results: High expression of UCP1 and UCP2 were positive prognostic markers for BC. UCP1 was associated with the impaired glucose metabolism, while UCP2 with enhanced anti-tumor immunity. High expressions of UCP1 and UCP2 were associated with CDH1 mutations. High UCP1 expression was associated with a high rate of thermogenesis in BC.Conclusions: These results implied a key role of UCP1 and UCP2 in prognosis, metabolism, and immune infiltration in BC. Further investigation of the relevant molecular mechanisms may provide new strategies for BC treatment.https://www.frontiersin.org/articles/10.3389/fcell.2022.891731/fullbreast canceruncoupling proteintumor immunitytumor metabolismthermal tomography |
| spellingShingle | Xin Yu Manman Shi Qi Wu Qi Wu Wen Wei Shengrong Sun Shan Zhu Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics Frontiers in Cell and Developmental Biology breast cancer uncoupling protein tumor immunity tumor metabolism thermal tomography |
| title | Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics |
| title_full | Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics |
| title_fullStr | Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics |
| title_full_unstemmed | Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics |
| title_short | Identification of UCP1 and UCP2 as Potential Prognostic Markers in Breast Cancer: A Study Based on Immunohistochemical Analysis and Bioinformatics |
| title_sort | identification of ucp1 and ucp2 as potential prognostic markers in breast cancer a study based on immunohistochemical analysis and bioinformatics |
| topic | breast cancer uncoupling protein tumor immunity tumor metabolism thermal tomography |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.891731/full |
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