Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings
Abstract Background The use of maggots, developed from chicken droppings as alternative fish meal might serve as a reservoir and vehicle for the widespread of single and multiple antibiotic resistant bacteria (MARB). This study aimed at investigating antibiotic resistant bacteria from chicken droppi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-02-01
|
| Series: | Environmental Sciences Europe |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12302-024-00840-w |
| _version_ | 1797275482280951808 |
|---|---|
| author | Olumide Alaba Ajibade Adebayonle Olayiwola Akinduro Gbajesu Omojufehinsi Babatunde Odetoyin Oladipo Oladiti Olaniyi |
| author_facet | Olumide Alaba Ajibade Adebayonle Olayiwola Akinduro Gbajesu Omojufehinsi Babatunde Odetoyin Oladipo Oladiti Olaniyi |
| author_sort | Olumide Alaba Ajibade |
| collection | DOAJ |
| description | Abstract Background The use of maggots, developed from chicken droppings as alternative fish meal might serve as a reservoir and vehicle for the widespread of single and multiple antibiotic resistant bacteria (MARB). This study aimed at investigating antibiotic resistant bacteria from chicken droppings and maggots developed from them, and the associated resistance genes and mobile genetic elements in their genomes. Freshly collected poultry droppings obtained from three commercial poultry farms, in Akure, Nigeria and maggots developed from their composting were plated on Luria–Bertani (LB) agar plates that had been incorporated with antibiotics. The emerged MARB were presumptively identified by conventional cultural microbiological methods and then authenticated using 16S rRNA gene sequence analysis. The antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in the genome of MARB were detected by standard molecular tools. Results Potential bacterial pathogens isolated include Escherichia coli, Bacillus cereus, Salmonella enterica, Providencia stuartii, P. rettgeri, Pseudomonas aeruginosa, Staphylococcus aureus, Alcaligenes faecalis, Paelnalcaligenes hominis and Micrococcus luteus. Seventeen ARGs were widely distributed among the 19 ARB/MARB. Tetracycline resistance gene was widely distributed among the ARB/MARB with prevalence of 62%, while macrolides and beta lactam resistance gene had 17% and 13%, respectively. At least six MARB isolates showed the presence of two or more class 1 integron, while five isolates was detected to carry two or more class 2 integron among the 19 MARB. Conclusions The results highlight a much higher risk of ARGs spreading through maggots derived from chicken droppings to humans and environmental microbiota. |
| first_indexed | 2024-03-07T15:15:00Z |
| format | Article |
| id | doaj.art-60781a96323e4230913aa7e192b73ead |
| institution | Directory Open Access Journal |
| issn | 2190-4715 |
| language | English |
| last_indexed | 2024-03-07T15:15:00Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Environmental Sciences Europe |
| spelling | doaj.art-60781a96323e4230913aa7e192b73ead2024-03-05T17:58:25ZengSpringerOpenEnvironmental Sciences Europe2190-47152024-02-0136111510.1186/s12302-024-00840-wMolecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppingsOlumide Alaba Ajibade0Adebayonle Olayiwola Akinduro1Gbajesu OmojufehinsiBabatunde Odetoyin2Oladipo Oladiti Olaniyi3Department of Microbiology, Federal University of TechnologyDepartment of Microbiology, Federal University of TechnologyDepartment of Medical Microbiology and Parasitology, Obafemi Awolowo UniversityDepartment of Microbiology, Federal University of TechnologyAbstract Background The use of maggots, developed from chicken droppings as alternative fish meal might serve as a reservoir and vehicle for the widespread of single and multiple antibiotic resistant bacteria (MARB). This study aimed at investigating antibiotic resistant bacteria from chicken droppings and maggots developed from them, and the associated resistance genes and mobile genetic elements in their genomes. Freshly collected poultry droppings obtained from three commercial poultry farms, in Akure, Nigeria and maggots developed from their composting were plated on Luria–Bertani (LB) agar plates that had been incorporated with antibiotics. The emerged MARB were presumptively identified by conventional cultural microbiological methods and then authenticated using 16S rRNA gene sequence analysis. The antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in the genome of MARB were detected by standard molecular tools. Results Potential bacterial pathogens isolated include Escherichia coli, Bacillus cereus, Salmonella enterica, Providencia stuartii, P. rettgeri, Pseudomonas aeruginosa, Staphylococcus aureus, Alcaligenes faecalis, Paelnalcaligenes hominis and Micrococcus luteus. Seventeen ARGs were widely distributed among the 19 ARB/MARB. Tetracycline resistance gene was widely distributed among the ARB/MARB with prevalence of 62%, while macrolides and beta lactam resistance gene had 17% and 13%, respectively. At least six MARB isolates showed the presence of two or more class 1 integron, while five isolates was detected to carry two or more class 2 integron among the 19 MARB. Conclusions The results highlight a much higher risk of ARGs spreading through maggots derived from chicken droppings to humans and environmental microbiota.https://doi.org/10.1186/s12302-024-00840-wMaggotsChicken fecesAntimicrobial susceptibilityAntibiotic resistanceAntibiotic resistance genes |
| spellingShingle | Olumide Alaba Ajibade Adebayonle Olayiwola Akinduro Gbajesu Omojufehinsi Babatunde Odetoyin Oladipo Oladiti Olaniyi Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings Environmental Sciences Europe Maggots Chicken feces Antimicrobial susceptibility Antibiotic resistance Antibiotic resistance genes |
| title | Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings |
| title_full | Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings |
| title_fullStr | Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings |
| title_full_unstemmed | Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings |
| title_short | Molecular characterization of antibiotic-resistant bacteria associated with maggots obtained from chicken droppings |
| title_sort | molecular characterization of antibiotic resistant bacteria associated with maggots obtained from chicken droppings |
| topic | Maggots Chicken feces Antimicrobial susceptibility Antibiotic resistance Antibiotic resistance genes |
| url | https://doi.org/10.1186/s12302-024-00840-w |
| work_keys_str_mv | AT olumidealabaajibade molecularcharacterizationofantibioticresistantbacteriaassociatedwithmaggotsobtainedfromchickendroppings AT adebayonleolayiwolaakinduro molecularcharacterizationofantibioticresistantbacteriaassociatedwithmaggotsobtainedfromchickendroppings AT gbajesuomojufehinsi molecularcharacterizationofantibioticresistantbacteriaassociatedwithmaggotsobtainedfromchickendroppings AT babatundeodetoyin molecularcharacterizationofantibioticresistantbacteriaassociatedwithmaggotsobtainedfromchickendroppings AT oladipooladitiolaniyi molecularcharacterizationofantibioticresistantbacteriaassociatedwithmaggotsobtainedfromchickendroppings |