New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching
As plant-specific transcription factors, the TIFY family genes are involved in the responses to a series of biotic and abiotic stresses and the regulation of the development of multiple organs. To explore the potential roles of the TIFY gene family in shoot branching, which can shape plant architect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/17114 |
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| author | Yarong Li Qian Zhang Luman Wang Xinfa Wang Jiangwei Qiao Hanzhong Wang |
| author_facet | Yarong Li Qian Zhang Luman Wang Xinfa Wang Jiangwei Qiao Hanzhong Wang |
| author_sort | Yarong Li |
| collection | DOAJ |
| description | As plant-specific transcription factors, the TIFY family genes are involved in the responses to a series of biotic and abiotic stresses and the regulation of the development of multiple organs. To explore the potential roles of the TIFY gene family in shoot branching, which can shape plant architecture and finally determine seed yield, we conducted comprehensive genome-wide analyses of the TIFY gene family in <i>Brassica napus</i>. Here, HMMER search and BLASTp were used to identify the TIFY members. A total of 70 TIFY members were identified and divided into four subfamilies based on the conserved domains and motifs. These TIFY genes were distributed across 19 chromosomes. The predicted subcellular localizations revealed that most TIFY proteins were located in the nucleus. The tissue expression profile analyses indicated that TIFY genes were highly expressed in the stem, flower bud, and silique at the transcriptional level. High-proportioned activation of the dormant axillary buds on stems determined the branch numbers of rapeseed plants. Here, transcriptome analyses were conducted on axillary buds in four sequential developing stages, that is, dormant, temporarily dormant, being activated, and elongating (already activated). Surprisingly, the transcription of the majority of TIFY genes (65 of the 70) significantly decreased on the activation of buds. GO enrichment analysis and hormone treatments indicated that the transcription of TIFY family genes can be strongly induced by jasmonic acid, implying that the TIFY family genes may be involved in the regulation of jasmonic acid-mediated branch development. These results shed light on the roles of TIFY family genes in plant architecture. |
| first_indexed | 2024-03-09T01:48:52Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:48:52Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-607a50f70c7e4bb1af338459aa61fd822023-12-08T15:18:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124231711410.3390/ijms242317114New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot BranchingYarong Li0Qian Zhang1Luman Wang2Xinfa Wang3Jiangwei Qiao4Hanzhong Wang5Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLaboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLaboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLaboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLaboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaLaboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chines Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, ChinaAs plant-specific transcription factors, the TIFY family genes are involved in the responses to a series of biotic and abiotic stresses and the regulation of the development of multiple organs. To explore the potential roles of the TIFY gene family in shoot branching, which can shape plant architecture and finally determine seed yield, we conducted comprehensive genome-wide analyses of the TIFY gene family in <i>Brassica napus</i>. Here, HMMER search and BLASTp were used to identify the TIFY members. A total of 70 TIFY members were identified and divided into four subfamilies based on the conserved domains and motifs. These TIFY genes were distributed across 19 chromosomes. The predicted subcellular localizations revealed that most TIFY proteins were located in the nucleus. The tissue expression profile analyses indicated that TIFY genes were highly expressed in the stem, flower bud, and silique at the transcriptional level. High-proportioned activation of the dormant axillary buds on stems determined the branch numbers of rapeseed plants. Here, transcriptome analyses were conducted on axillary buds in four sequential developing stages, that is, dormant, temporarily dormant, being activated, and elongating (already activated). Surprisingly, the transcription of the majority of TIFY genes (65 of the 70) significantly decreased on the activation of buds. GO enrichment analysis and hormone treatments indicated that the transcription of TIFY family genes can be strongly induced by jasmonic acid, implying that the TIFY family genes may be involved in the regulation of jasmonic acid-mediated branch development. These results shed light on the roles of TIFY family genes in plant architecture.https://www.mdpi.com/1422-0067/24/23/17114<i>Brassica napus</i>TIFY gene familyaxillary budsshoot branchingjasmonic acid |
| spellingShingle | Yarong Li Qian Zhang Luman Wang Xinfa Wang Jiangwei Qiao Hanzhong Wang New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching International Journal of Molecular Sciences <i>Brassica napus</i> TIFY gene family axillary buds shoot branching jasmonic acid |
| title | New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching |
| title_full | New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching |
| title_fullStr | New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching |
| title_full_unstemmed | New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching |
| title_short | New Insights into the TIFY Gene Family of <i>Brassica napus</i> and Its Involvement in the Regulation of Shoot Branching |
| title_sort | new insights into the tify gene family of i brassica napus i and its involvement in the regulation of shoot branching |
| topic | <i>Brassica napus</i> TIFY gene family axillary buds shoot branching jasmonic acid |
| url | https://www.mdpi.com/1422-0067/24/23/17114 |
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