Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1411469 |
| Summary: | A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples. |
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| ISSN: | 0954-0105 1465-3443 |