Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine
A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1411469 |
| _version_ | 1819039740585836544 |
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| author | Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang |
| author_facet | Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang |
| author_sort | Wei Jiang |
| collection | DOAJ |
| description | A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples. |
| first_indexed | 2024-12-21T08:58:01Z |
| format | Article |
| id | doaj.art-607b9a049401496bb02ca1489481fe96 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-21T08:58:01Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-607b9a049401496bb02ca1489481fe962022-12-21T19:09:30ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129153654710.1080/09540105.2017.14114691411469Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urineWei Jiang0Lu Zeng1Liqiang Liu2Shanshan Song3Hua Kuang4State Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, Jiangnan UniversityA clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples.http://dx.doi.org/10.1080/09540105.2017.1411469clorprenalineantibodypig urineimmunochromatographic strip |
| spellingShingle | Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine Food and Agricultural Immunology clorprenaline antibody pig urine immunochromatographic strip |
| title | Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| title_full | Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| title_fullStr | Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| title_full_unstemmed | Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| title_short | Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| title_sort | development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine |
| topic | clorprenaline antibody pig urine immunochromatographic strip |
| url | http://dx.doi.org/10.1080/09540105.2017.1411469 |
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