The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran
Background: The D antigen is a subset of Rh blood group antigens involved in the hemolytic disease of the newborn [HDFN] and hemolytic transfusion reaction [HTR]. The hybrid Rhesus box that was created after RH gene deletion, was known as a mechanism of the Rh-negative phenotype. Hybrid marker ident...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-10-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1335 |
| _version_ | 1827849977421889536 |
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| author | Younes Sadeghi-Bojd Naser Amirizadeh Arezoo Oodi |
| author_facet | Younes Sadeghi-Bojd Naser Amirizadeh Arezoo Oodi |
| author_sort | Younes Sadeghi-Bojd |
| collection | DOAJ |
| description | Background: The D antigen is a subset of Rh blood group antigens involved in the hemolytic disease of the newborn [HDFN] and hemolytic transfusion reaction [HTR]. The hybrid Rhesus box that was created after RH gene deletion, was known as a mechanism of the Rh-negative phenotype. Hybrid marker identification is used to confirm the deletion of the RHD gene and to determine zygosity. This study aims to detect this marker in Rh-negative and weak D phenotype blood donors of the southeast of Iran.
Materials and Methods: The molecular analysis of the hybrid Rhesus box was performed on the 200 Rh-negative blood donors in Sistan and Baluchestan province, southeast Iran. The presence of alleles responsible for the D variants was assessed by DNA sequencing in 26 weak D phenotype donors.
Results: Of the 200 Rh-negative blood samples, 198 samples were homozygous (99%), and two samples were heterozygous (1%). Heterozygous samples had RHD*01N.73 allele and the RHD*01N.18 allele. Of the 26 samples with weak D phenotype, 16 partial DLO (61%), 4 partial DBT1 (15.3%), 2 partial DV type 2 (7.7%), 1 weak D type 1, 1 weak D type 4.2.3, 1weak D type 105 and 1 RHD (S103P) (4%) were determined.
Conclusion: Since RHD gene deletion is the main mechanism of the Rh-negativity in Sistan and Baluchestan provinces, a hybrid Rhesus box marker can be used in resolving RhD typing discrepancies by RHD genotyping methods. |
| first_indexed | 2024-03-12T10:12:41Z |
| format | Article |
| id | doaj.art-607e971b9fa046fa88097ca840f6b532 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T10:12:41Z |
| publishDate | 2021-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-607e971b9fa046fa88097ca840f6b5322023-09-02T10:43:30ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072021-10-0115410.18502/ijhoscr.v15i4.7476The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast IranYounes Sadeghi-Bojd0Naser Amirizadeh1Arezoo Oodi2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBackground: The D antigen is a subset of Rh blood group antigens involved in the hemolytic disease of the newborn [HDFN] and hemolytic transfusion reaction [HTR]. The hybrid Rhesus box that was created after RH gene deletion, was known as a mechanism of the Rh-negative phenotype. Hybrid marker identification is used to confirm the deletion of the RHD gene and to determine zygosity. This study aims to detect this marker in Rh-negative and weak D phenotype blood donors of the southeast of Iran. Materials and Methods: The molecular analysis of the hybrid Rhesus box was performed on the 200 Rh-negative blood donors in Sistan and Baluchestan province, southeast Iran. The presence of alleles responsible for the D variants was assessed by DNA sequencing in 26 weak D phenotype donors. Results: Of the 200 Rh-negative blood samples, 198 samples were homozygous (99%), and two samples were heterozygous (1%). Heterozygous samples had RHD*01N.73 allele and the RHD*01N.18 allele. Of the 26 samples with weak D phenotype, 16 partial DLO (61%), 4 partial DBT1 (15.3%), 2 partial DV type 2 (7.7%), 1 weak D type 1, 1 weak D type 4.2.3, 1weak D type 105 and 1 RHD (S103P) (4%) were determined. Conclusion: Since RHD gene deletion is the main mechanism of the Rh-negativity in Sistan and Baluchestan provinces, a hybrid Rhesus box marker can be used in resolving RhD typing discrepancies by RHD genotyping methods.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1335Hybrid Rhesus box; D variant;RHD gene deletion; RhD-negative phenotype; Weak D |
| spellingShingle | Younes Sadeghi-Bojd Naser Amirizadeh Arezoo Oodi The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran International Journal of Hematology-Oncology and Stem Cell Research Hybrid Rhesus box; D variant;RHD gene deletion; RhD-negative phenotype; Weak D |
| title | The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran |
| title_full | The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran |
| title_fullStr | The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran |
| title_full_unstemmed | The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran |
| title_short | The RHD Genotyping of Rh-Negative and Weak D Phenotype among Blood Donors in Southeast Iran |
| title_sort | rhd genotyping of rh negative and weak d phenotype among blood donors in southeast iran |
| topic | Hybrid Rhesus box; D variant;RHD gene deletion; RhD-negative phenotype; Weak D |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1335 |
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