ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT
Objective: To investigate the effect of exposure to chlorpyryphos, carbofuran, and cypermethrin on spermatogenesis in adolescent rat. Material & Methods: This study is an experimental study with a post-test only control group design. In this study, 30 adolescent rats aged 30-45 days were random...
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| Format: | Article |
| Language: | English |
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IKATAN AHLI UROLOGI INDONESIA
2023-05-01
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| Series: | Jurnal Urologi Indonesia |
| Subjects: | |
| Online Access: | https://juri.urologi.or.id/juri/article/view/803 |
| _version_ | 1797831680659030016 |
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| author | Libri Wira Indra Janu Septa Surya Wahyudi Kristianningrum Dian Sofiana |
| author_facet | Libri Wira Indra Janu Septa Surya Wahyudi Kristianningrum Dian Sofiana |
| author_sort | Libri Wira Indra Janu |
| collection | DOAJ |
| description |
Objective: To investigate the effect of exposure to chlorpyryphos, carbofuran, and cypermethrin on spermatogenesis in adolescent rat. Material & Methods: This study is an experimental study with a post-test only control group design. In this study, 30 adolescent rats aged 30-45 days were randomly divided into 5 groups. Group I was a normal control or without treatment for 21 days (K0), group II was a solvent control group, namely DMSO 5% which was administered subcutaneously for 21 days (KI), group III was a group that received chlorpyrifos 20 mg/kgBW dissolved with DMSO 5% subcutaneously for 21 days (P1), group IV was the group that received carbofuran 0.2 mg/kgBW dissolved with DMSO 5% subcutaneously for 21 days (P2), group V was the group that received cypermethrin 20 mg/kg. kgBW dissolved with 5% DMSO subcutaneously for 21 days (P3). Ipsilateral testicular orchidectomy was performed on all rats to remove the left testicular organ for preparation with Haematoxylin Eosin staining. The spermatogenesis score was calculated based on the Johnsen score in 5 seminiferous tubule visual fields. Data were analyzed using Kruskal Wallis followed by Mann Mitney test. The difference was considered significant at p<0.05. Results: There was a significant decrease in spermatogenesis scores between P1, P2, and P3 against K0 and K1 (p<0.05). On the other hand, there was no significant difference in the decrease in spermatogenesis scores between P1 and P2, P1 and P3, P2 and P3 and K0 and K1 (p>0.05). Conclusion: Exposure to chlorpyrifos, carbofuran, and cypermethrin had an effect on reducing spermatogenesis scores in adolescent rats.
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| first_indexed | 2024-04-09T13:55:42Z |
| format | Article |
| id | doaj.art-607efdfbd23345f7809a6eac6d88fcf5 |
| institution | Directory Open Access Journal |
| issn | 0853-442X 2355-1402 |
| language | English |
| last_indexed | 2024-04-09T13:55:42Z |
| publishDate | 2023-05-01 |
| publisher | IKATAN AHLI UROLOGI INDONESIA |
| record_format | Article |
| series | Jurnal Urologi Indonesia |
| spelling | doaj.art-607efdfbd23345f7809a6eac6d88fcf52023-05-08T05:38:02ZengIKATAN AHLI UROLOGI INDONESIAJurnal Urologi Indonesia0853-442X2355-14022023-05-0130210.32421/juri.v30i2.803ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RATLibri Wira Indra Janu0Septa Surya Wahyudi1Kristianningrum Dian Sofiana2Faculty of Medicine/University of Jember, Jember. Staff of Medical Faculty, Faculty of Medicine/University of Jember, Jember.Staff of Medical Faculty, Faculty of Medicine/University of Jember, Jember. Objective: To investigate the effect of exposure to chlorpyryphos, carbofuran, and cypermethrin on spermatogenesis in adolescent rat. Material & Methods: This study is an experimental study with a post-test only control group design. In this study, 30 adolescent rats aged 30-45 days were randomly divided into 5 groups. Group I was a normal control or without treatment for 21 days (K0), group II was a solvent control group, namely DMSO 5% which was administered subcutaneously for 21 days (KI), group III was a group that received chlorpyrifos 20 mg/kgBW dissolved with DMSO 5% subcutaneously for 21 days (P1), group IV was the group that received carbofuran 0.2 mg/kgBW dissolved with DMSO 5% subcutaneously for 21 days (P2), group V was the group that received cypermethrin 20 mg/kg. kgBW dissolved with 5% DMSO subcutaneously for 21 days (P3). Ipsilateral testicular orchidectomy was performed on all rats to remove the left testicular organ for preparation with Haematoxylin Eosin staining. The spermatogenesis score was calculated based on the Johnsen score in 5 seminiferous tubule visual fields. Data were analyzed using Kruskal Wallis followed by Mann Mitney test. The difference was considered significant at p<0.05. Results: There was a significant decrease in spermatogenesis scores between P1, P2, and P3 against K0 and K1 (p<0.05). On the other hand, there was no significant difference in the decrease in spermatogenesis scores between P1 and P2, P1 and P3, P2 and P3 and K0 and K1 (p>0.05). Conclusion: Exposure to chlorpyrifos, carbofuran, and cypermethrin had an effect on reducing spermatogenesis scores in adolescent rats. https://juri.urologi.or.id/juri/article/view/803Chlorpyryphoscarbofurancypermethrinspermatogenesis |
| spellingShingle | Libri Wira Indra Janu Septa Surya Wahyudi Kristianningrum Dian Sofiana ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT Jurnal Urologi Indonesia Chlorpyryphos carbofuran cypermethrin spermatogenesis |
| title | ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT |
| title_full | ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT |
| title_fullStr | ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT |
| title_full_unstemmed | ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT |
| title_short | ANALYSIS OF THE EFFECT OF EXPOSURE TO CHLORPYRYPHOS, CARBOFURAN, AND CYPERMETHRIN ON SPERMATOGENESIS IN ADOLESCENT RAT |
| title_sort | analysis of the effect of exposure to chlorpyryphos carbofuran and cypermethrin on spermatogenesis in adolescent rat |
| topic | Chlorpyryphos carbofuran cypermethrin spermatogenesis |
| url | https://juri.urologi.or.id/juri/article/view/803 |
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