miR-708-5p Regulates Myoblast Proliferation and Differentiation
MicroRNAs (miRNAs) are key regulators involved in the myogenic process in skeletal muscles. miR-708-5p plays an important role in various biochemical and physiological processes, but its function in skeletal myogenesis remain unclear. In this study, we first explored the effects of miR-708-5p on C2C...
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MDPI AG
2022-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/11/641 |
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| author | Xueli Xu Hui Lu Dong Xu Zonggang Yu Nini Ai Kaiming Wang Xintong Li Jun He Jun Jiang Haiming Ma Yuebo Zhang |
| author_facet | Xueli Xu Hui Lu Dong Xu Zonggang Yu Nini Ai Kaiming Wang Xintong Li Jun He Jun Jiang Haiming Ma Yuebo Zhang |
| author_sort | Xueli Xu |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are key regulators involved in the myogenic process in skeletal muscles. miR-708-5p plays an important role in various biochemical and physiological processes, but its function in skeletal myogenesis remain unclear. In this study, we first explored the effects of miR-708-5p on C2C12 proliferation and differentiation by overexpression and interference experiments. Then, we predicted the target genes of miR-708-5p and analyzed their function. We found that miR-708-5p was gradually increased during myoblast differentiation. Overexpression of miR-708-5p significantly inhibited cell proliferation and promoted the differentiation of myoblasts. A total of 253 target genes were predicted using a bioinformatics approach. These genes were significantly enriched in muscle growth-related GO terms and KEGG pathways, such as actin filament organization, actin cytoskeleton organization, PI3K-Akt pathway, insulin pathway, and Jak-STAT pathway. Among them, <i>Pik3ca</i>, <i>Pik3r3</i>, and <i>Irs1</i> were considered to be the key target genes of miR-708-5p. To sum up, miR-708-5p inhibited C2C12 cells proliferation and promoted C2C12 cells differentiation. Its target genes significantly enriched in GO terms and KEGG pathways related to the development and growth of muscle. Our results provided a basis for studies on the function and mechanism of miR-708-5p regulating skeletal muscle growth and development. |
| first_indexed | 2024-03-09T17:56:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T17:56:11Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-607f2575a1ec45f4aa5a2484c1da30072023-11-24T10:17:52ZengMDPI AGVeterinary Sciences2306-73812022-11-0191164110.3390/vetsci9110641miR-708-5p Regulates Myoblast Proliferation and DifferentiationXueli Xu0Hui Lu1Dong Xu2Zonggang Yu3Nini Ai4Kaiming Wang5Xintong Li6Jun He7Jun Jiang8Haiming Ma9Yuebo Zhang10College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaBiological and Environmental Engineering, Yueyang Vocational Technical College, Yueyang 414000, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaMicroRNAs (miRNAs) are key regulators involved in the myogenic process in skeletal muscles. miR-708-5p plays an important role in various biochemical and physiological processes, but its function in skeletal myogenesis remain unclear. In this study, we first explored the effects of miR-708-5p on C2C12 proliferation and differentiation by overexpression and interference experiments. Then, we predicted the target genes of miR-708-5p and analyzed their function. We found that miR-708-5p was gradually increased during myoblast differentiation. Overexpression of miR-708-5p significantly inhibited cell proliferation and promoted the differentiation of myoblasts. A total of 253 target genes were predicted using a bioinformatics approach. These genes were significantly enriched in muscle growth-related GO terms and KEGG pathways, such as actin filament organization, actin cytoskeleton organization, PI3K-Akt pathway, insulin pathway, and Jak-STAT pathway. Among them, <i>Pik3ca</i>, <i>Pik3r3</i>, and <i>Irs1</i> were considered to be the key target genes of miR-708-5p. To sum up, miR-708-5p inhibited C2C12 cells proliferation and promoted C2C12 cells differentiation. Its target genes significantly enriched in GO terms and KEGG pathways related to the development and growth of muscle. Our results provided a basis for studies on the function and mechanism of miR-708-5p regulating skeletal muscle growth and development.https://www.mdpi.com/2306-7381/9/11/641miR-708-5pC2C12 cellsproliferationdifferentiationtarget genes |
| spellingShingle | Xueli Xu Hui Lu Dong Xu Zonggang Yu Nini Ai Kaiming Wang Xintong Li Jun He Jun Jiang Haiming Ma Yuebo Zhang miR-708-5p Regulates Myoblast Proliferation and Differentiation Veterinary Sciences miR-708-5p C2C12 cells proliferation differentiation target genes |
| title | miR-708-5p Regulates Myoblast Proliferation and Differentiation |
| title_full | miR-708-5p Regulates Myoblast Proliferation and Differentiation |
| title_fullStr | miR-708-5p Regulates Myoblast Proliferation and Differentiation |
| title_full_unstemmed | miR-708-5p Regulates Myoblast Proliferation and Differentiation |
| title_short | miR-708-5p Regulates Myoblast Proliferation and Differentiation |
| title_sort | mir 708 5p regulates myoblast proliferation and differentiation |
| topic | miR-708-5p C2C12 cells proliferation differentiation target genes |
| url | https://www.mdpi.com/2306-7381/9/11/641 |
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