Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens
The objective of this study was to investigate the effects of tributyrin supplementation on liver fat metabolism in broiler chickens. Two hundred and forty broilers were randomly allocated into two experimental groups (6 replicates per treatment; 20 chickens in each replicate): the control group (CN...
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MDPI AG
2022-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/12/2219 |
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| author | Tiantian Gu Mingcai Duan Jinyu Liu Li Chen Yong Tian Wenwu Xu Tao Zeng Lizhi Lu |
| author_facet | Tiantian Gu Mingcai Duan Jinyu Liu Li Chen Yong Tian Wenwu Xu Tao Zeng Lizhi Lu |
| author_sort | Tiantian Gu |
| collection | DOAJ |
| description | The objective of this study was to investigate the effects of tributyrin supplementation on liver fat metabolism in broiler chickens. Two hundred and forty broilers were randomly allocated into two experimental groups (6 replicates per treatment; 20 chickens in each replicate): the control group (CN), which received a basal diet, and the tributyrin group (TB), which received a basal diet supplemented with 1 g/kg of tributyrin. The experimental period lasted 37 days. The results showed that in the liver, broilers supplemented with tributyrin had higher content of high-density lipoprotein cholesterol (HDL-C) (<i>p</i> < 0.05). Liver hepatic lipase (HL), lipoprotein lipase (LPL) and total lipid (TL) activity were significantly lower than in the TB group than that in the NC group. Meanwhile, the diet supplemented with tributyrin had more lipid droplets than the NC group, whereas the TB and NC groups showed no histological abnormalities in the liver. Furthermore, the mRNA expression levels of peroxisome proliferators-activated receptor α (<i>PPARα</i>), proliferators-activated receptor γ (<i>PPARγ</i>), fatty acid synthase (<i>FAS</i>), <i>LPL</i> and adipose triglyceride lipase (<i>ATGL</i>) in the liver were significantly upregulated in the TB group (<i>p</i> < 0.05), while those of the long-chain acyl-CoA-synthetase 1 (<i>ACSL1</i>) mRNA between the TB group and the NC group were not different (<i>p</i> > 0.05). These findings indicated that the diet supplemented with tributyrin could increase fat deposition appropriately by promoting fat synthesis without causing liver tissue damage, which demonstrated that tributyrin can be considered a valid feed additive for broiler chickens. |
| first_indexed | 2024-03-09T16:30:24Z |
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| id | doaj.art-60821977df0640c7b843908208e3300c |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:30:24Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-60821977df0640c7b843908208e3300c2023-11-24T15:03:01ZengMDPI AGGenes2073-44252022-11-011312221910.3390/genes13122219Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler ChickensTiantian Gu0Mingcai Duan1Jinyu Liu2Li Chen3Yong Tian4Wenwu Xu5Tao Zeng6Lizhi Lu7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe objective of this study was to investigate the effects of tributyrin supplementation on liver fat metabolism in broiler chickens. Two hundred and forty broilers were randomly allocated into two experimental groups (6 replicates per treatment; 20 chickens in each replicate): the control group (CN), which received a basal diet, and the tributyrin group (TB), which received a basal diet supplemented with 1 g/kg of tributyrin. The experimental period lasted 37 days. The results showed that in the liver, broilers supplemented with tributyrin had higher content of high-density lipoprotein cholesterol (HDL-C) (<i>p</i> < 0.05). Liver hepatic lipase (HL), lipoprotein lipase (LPL) and total lipid (TL) activity were significantly lower than in the TB group than that in the NC group. Meanwhile, the diet supplemented with tributyrin had more lipid droplets than the NC group, whereas the TB and NC groups showed no histological abnormalities in the liver. Furthermore, the mRNA expression levels of peroxisome proliferators-activated receptor α (<i>PPARα</i>), proliferators-activated receptor γ (<i>PPARγ</i>), fatty acid synthase (<i>FAS</i>), <i>LPL</i> and adipose triglyceride lipase (<i>ATGL</i>) in the liver were significantly upregulated in the TB group (<i>p</i> < 0.05), while those of the long-chain acyl-CoA-synthetase 1 (<i>ACSL1</i>) mRNA between the TB group and the NC group were not different (<i>p</i> > 0.05). These findings indicated that the diet supplemented with tributyrin could increase fat deposition appropriately by promoting fat synthesis without causing liver tissue damage, which demonstrated that tributyrin can be considered a valid feed additive for broiler chickens.https://www.mdpi.com/2073-4425/13/12/2219tributyrinfat depositionlipid metabolismgene expressionbroiler chicken |
| spellingShingle | Tiantian Gu Mingcai Duan Jinyu Liu Li Chen Yong Tian Wenwu Xu Tao Zeng Lizhi Lu Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens Genes tributyrin fat deposition lipid metabolism gene expression broiler chicken |
| title | Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens |
| title_full | Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens |
| title_fullStr | Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens |
| title_full_unstemmed | Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens |
| title_short | Effects of Tributyrin Supplementation on Liver Fat Deposition, Lipid Levels and Lipid Metabolism-Related Gene Expression in Broiler Chickens |
| title_sort | effects of tributyrin supplementation on liver fat deposition lipid levels and lipid metabolism related gene expression in broiler chickens |
| topic | tributyrin fat deposition lipid metabolism gene expression broiler chicken |
| url | https://www.mdpi.com/2073-4425/13/12/2219 |
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