Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane
The ability to expand crop plantations without irrigation is a major goal to increase agriculture sustainability. To achieve this end, we need to understand the mechanisms that govern plant growth responses under drought conditions. In this study, we combined physiological, transcriptomic, and genom...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9124 |
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| author | Augusto Lima Diniz Danielle Izilda Rodrigues da Silva Carolina Gimiliani Lembke Maximiller Dal-Bianco Lamas Costa Felipe ten-Caten Forrest Li Romel Duarte Vilela Marcelo Menossi Doreen Ware Lauricio Endres Glaucia Mendes Souza |
| author_facet | Augusto Lima Diniz Danielle Izilda Rodrigues da Silva Carolina Gimiliani Lembke Maximiller Dal-Bianco Lamas Costa Felipe ten-Caten Forrest Li Romel Duarte Vilela Marcelo Menossi Doreen Ware Lauricio Endres Glaucia Mendes Souza |
| author_sort | Augusto Lima Diniz |
| collection | DOAJ |
| description | The ability to expand crop plantations without irrigation is a major goal to increase agriculture sustainability. To achieve this end, we need to understand the mechanisms that govern plant growth responses under drought conditions. In this study, we combined physiological, transcriptomic, and genomic data to provide a comprehensive picture of drought and recovery responses in the leaves and roots of sugarcane. Transcriptomic profiling using oligoarrays and RNA-seq identified 2898 (out of 21,902) and 46,062 (out of 373,869) transcripts as differentially expressed, respectively. Co-expression analysis revealed modules enriched in photosynthesis, small molecule metabolism, alpha-amino acid metabolism, trehalose biosynthesis, serine family amino acid metabolism, and carbohydrate transport. Together, our findings reveal that carbohydrate metabolism is coordinated with the degradation of amino acids to provide carbon skeletons to the tricarboxylic acid cycle. This coordination may help to maintain energetic balance during drought stress adaptation, facilitating recovery after the stress is alleviated. Our results shed light on candidate regulatory elements and pave the way to biotechnology strategies towards the development of drought-tolerant sugarcane plants. |
| first_indexed | 2024-03-10T14:25:12Z |
| format | Article |
| id | doaj.art-608351524af44fa5beaf85af86853268 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:25:12Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-608351524af44fa5beaf85af868532682023-11-20T23:01:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012123912410.3390/ijms21239124Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in SugarcaneAugusto Lima Diniz0Danielle Izilda Rodrigues da Silva1Carolina Gimiliani Lembke2Maximiller Dal-Bianco Lamas Costa3Felipe ten-Caten4Forrest Li5Romel Duarte Vilela6Marcelo Menossi7Doreen Ware8Lauricio Endres9Glaucia Mendes Souza10Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilCold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USACentro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL 57100-000, BrazilInstituto de Biologia, Universidade Estadual de Campinas, Campinas, SP 13083-862, BrazilCold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USACentro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL 57100-000, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilThe ability to expand crop plantations without irrigation is a major goal to increase agriculture sustainability. To achieve this end, we need to understand the mechanisms that govern plant growth responses under drought conditions. In this study, we combined physiological, transcriptomic, and genomic data to provide a comprehensive picture of drought and recovery responses in the leaves and roots of sugarcane. Transcriptomic profiling using oligoarrays and RNA-seq identified 2898 (out of 21,902) and 46,062 (out of 373,869) transcripts as differentially expressed, respectively. Co-expression analysis revealed modules enriched in photosynthesis, small molecule metabolism, alpha-amino acid metabolism, trehalose biosynthesis, serine family amino acid metabolism, and carbohydrate transport. Together, our findings reveal that carbohydrate metabolism is coordinated with the degradation of amino acids to provide carbon skeletons to the tricarboxylic acid cycle. This coordination may help to maintain energetic balance during drought stress adaptation, facilitating recovery after the stress is alleviated. Our results shed light on candidate regulatory elements and pave the way to biotechnology strategies towards the development of drought-tolerant sugarcane plants.https://www.mdpi.com/1422-0067/21/23/9124plant stresstranscriptomeco-expression networktricarboxylic acid cycleTFBS |
| spellingShingle | Augusto Lima Diniz Danielle Izilda Rodrigues da Silva Carolina Gimiliani Lembke Maximiller Dal-Bianco Lamas Costa Felipe ten-Caten Forrest Li Romel Duarte Vilela Marcelo Menossi Doreen Ware Lauricio Endres Glaucia Mendes Souza Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane International Journal of Molecular Sciences plant stress transcriptome co-expression network tricarboxylic acid cycle TFBS |
| title | Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane |
| title_full | Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane |
| title_fullStr | Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane |
| title_full_unstemmed | Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane |
| title_short | Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane |
| title_sort | amino acid and carbohydrate metabolism are coordinated to maintain energetic balance during drought in sugarcane |
| topic | plant stress transcriptome co-expression network tricarboxylic acid cycle TFBS |
| url | https://www.mdpi.com/1422-0067/21/23/9124 |
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