Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum
Potassium has an important role to play in multiple cellular processes. In Saccharomyces cerevisiae, the serine/threonine (S/T) kinase Sat4/Hal4 is required for potassium accumulation, and thus, regulates the resistance to sodium salts and helps in the stabilization of other plasma membrane transpor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02311/full |
| _version_ | 1818570680826855424 |
|---|---|
| author | Ji-Yun Yang Yu-Lan Fang Ping Wang Jian-Ren Ye Lin Huang |
| author_facet | Ji-Yun Yang Yu-Lan Fang Ping Wang Jian-Ren Ye Lin Huang |
| author_sort | Ji-Yun Yang |
| collection | DOAJ |
| description | Potassium has an important role to play in multiple cellular processes. In Saccharomyces cerevisiae, the serine/threonine (S/T) kinase Sat4/Hal4 is required for potassium accumulation, and thus, regulates the resistance to sodium salts and helps in the stabilization of other plasma membrane transporters. However, the functions of Sat4 in filamentous phytopathogenic fungi are largely unknown. In this study, ChSat4, the yeast Sat4p homolog in Colletotrichum higginsianum, has been identified. Target deletion of ChSAT4 resulted in defects in mycelial growth and sporulation. Intracellular K+ accumulation was significantly decreased in the ChSAT4 deletion mutant. Additionally, the ΔChsat4 mutant showed defects in cell wall integrity, hyperoxide stress response, and pathogenicity. Localization pattern analysis indicated ChSat4 was localized in the cytoplasm. Furthermore, ChSat4 showed high functional conservation with the homolog FgSat4 in Fusarium graminearum. Taken together, our data indicated that ChSat4 was important for intracellular K+ accumulation and infection morphogenesis in C. higginsianum. |
| first_indexed | 2024-12-14T13:45:08Z |
| format | Article |
| id | doaj.art-6087002311204b93bbb027f87e37cd92 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T13:45:08Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6087002311204b93bbb027f87e37cd922022-12-21T22:59:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02311386278Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianumJi-Yun YangYu-Lan FangPing WangJian-Ren YeLin HuangPotassium has an important role to play in multiple cellular processes. In Saccharomyces cerevisiae, the serine/threonine (S/T) kinase Sat4/Hal4 is required for potassium accumulation, and thus, regulates the resistance to sodium salts and helps in the stabilization of other plasma membrane transporters. However, the functions of Sat4 in filamentous phytopathogenic fungi are largely unknown. In this study, ChSat4, the yeast Sat4p homolog in Colletotrichum higginsianum, has been identified. Target deletion of ChSAT4 resulted in defects in mycelial growth and sporulation. Intracellular K+ accumulation was significantly decreased in the ChSAT4 deletion mutant. Additionally, the ΔChsat4 mutant showed defects in cell wall integrity, hyperoxide stress response, and pathogenicity. Localization pattern analysis indicated ChSat4 was localized in the cytoplasm. Furthermore, ChSat4 showed high functional conservation with the homolog FgSat4 in Fusarium graminearum. Taken together, our data indicated that ChSat4 was important for intracellular K+ accumulation and infection morphogenesis in C. higginsianum.https://www.frontiersin.org/article/10.3389/fmicb.2018.02311/fullColletotrichum higginsianumserine/threonine kinaseK+ accumulationcell wall integritypathogenicity |
| spellingShingle | Ji-Yun Yang Yu-Lan Fang Ping Wang Jian-Ren Ye Lin Huang Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum Frontiers in Microbiology Colletotrichum higginsianum serine/threonine kinase K+ accumulation cell wall integrity pathogenicity |
| title | Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum |
| title_full | Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum |
| title_fullStr | Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum |
| title_full_unstemmed | Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum |
| title_short | Pleiotropic Roles of ChSat4 in Asexual Development, Cell Wall Integrity Maintenance, and Pathogenicity in Colletotrichum higginsianum |
| title_sort | pleiotropic roles of chsat4 in asexual development cell wall integrity maintenance and pathogenicity in colletotrichum higginsianum |
| topic | Colletotrichum higginsianum serine/threonine kinase K+ accumulation cell wall integrity pathogenicity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02311/full |
| work_keys_str_mv | AT jiyunyang pleiotropicrolesofchsat4inasexualdevelopmentcellwallintegritymaintenanceandpathogenicityincolletotrichumhigginsianum AT yulanfang pleiotropicrolesofchsat4inasexualdevelopmentcellwallintegritymaintenanceandpathogenicityincolletotrichumhigginsianum AT pingwang pleiotropicrolesofchsat4inasexualdevelopmentcellwallintegritymaintenanceandpathogenicityincolletotrichumhigginsianum AT jianrenye pleiotropicrolesofchsat4inasexualdevelopmentcellwallintegritymaintenanceandpathogenicityincolletotrichumhigginsianum AT linhuang pleiotropicrolesofchsat4inasexualdevelopmentcellwallintegritymaintenanceandpathogenicityincolletotrichumhigginsianum |