Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations
The objective of this work was to characterize the phytochemical composition of essential oil from Juniperus thurifera (L.) fruits (EOFT) and study its antioxidant, antibacterial, and antifungal effects. EOFT was extracted by hydrodistillation and fingerprinted by using GC–MS. The antioxidant effect...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-05-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2022-0332 |
| _version_ | 1797814207492653056 |
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| author | Lafraxo Soufyane Zouirech Otmane El Barnossi Azeddin Chelouati Tarik Chebaibi Mohamed Chebbac Khalid Nafidi Hiba-Allah Salamatullah Ahmad Mohammad Bourhia Mohammed Aboul-Soud Mourad A. M. Bari Amina |
| author_facet | Lafraxo Soufyane Zouirech Otmane El Barnossi Azeddin Chelouati Tarik Chebaibi Mohamed Chebbac Khalid Nafidi Hiba-Allah Salamatullah Ahmad Mohammad Bourhia Mohammed Aboul-Soud Mourad A. M. Bari Amina |
| author_sort | Lafraxo Soufyane |
| collection | DOAJ |
| description | The objective of this work was to characterize the phytochemical composition of essential oil from Juniperus thurifera (L.) fruits (EOFT) and study its antioxidant, antibacterial, and antifungal effects. EOFT was extracted by hydrodistillation and fingerprinted by using GC–MS. The antioxidant effect of EOFT was evaluated using 2,2-diphenylpicrylhydrazyl (DPPH), ferric iron reduction assay (FRAP), and total antioxidant capacity (TAC) assays. Importantly, the antimicrobial activity of EOFT was performed against Candida albicans, Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Proteus mirabilis. In addition, the inhibitory capacity of NADPH oxidase and human acetylcholinesterase was also investigated using molecular docking. The results of the chemical composition reveal that EOFT constituted 11 terpenic compounds with dominance of elemol (33.86%), terpinen-4-ol (27.80%), and cryptomeridiol (18.36%). The antioxidant power of EOFT recorded IC50 values of 197.07 ± 0.09 μg/mL (DPPH) and 216.34 ± 0.06 μg/mL (FRAP), while TAC of EOFT was determined to be 181.06 μg AAE/mg. The antibacterial potency on solid medium revealed that EOFT induced inhibition zone diameters reaching 14 mm, and a minimum concentration up to 2.78 µg/mL against the studied bacterial strains. The EOFT also showed an important antifungal effect as the inhibition reached 42%, and the MIC was between 7.50 and 22.25 µg/mL. The in silico study showed that o-Cymene was the most active molecule against NAD(P)H oxidase followed by cadinol with a Glide score of −5.344 and −5.143 kcal/mol, respectively. Due to their promising results, the outcome of this work suggests that EOFT could be used as an interesting natural weapon to control microbial and freed radical-related diseases. |
| first_indexed | 2024-03-13T08:04:12Z |
| format | Article |
| id | doaj.art-608b32afa408448ca4b2c1e3917de63c |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-03-13T08:04:12Z |
| publishDate | 2023-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-608b32afa408448ca4b2c1e3917de63c2023-06-01T09:42:09ZengDe GruyterOpen Chemistry2391-54202023-05-012116184010.1515/chem-2022-0332Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigationsLafraxo Soufyane0Zouirech Otmane1El Barnossi Azeddin2Chelouati Tarik3Chebaibi Mohamed4Chebbac Khalid5Nafidi Hiba-Allah6Salamatullah Ahmad Mohammad7Bourhia Mohammed8Aboul-Soud Mourad A. M.9Bari Amina10Laboratory of Biotechnology and Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), 30000Fez, MoroccoLaboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Biotechnology and Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), 30000Fez, MoroccoLaboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Biomedical and Translational Research, Faculty of Medicine and Pharmacy of Fez, University of Sidi Mohamed Ben Abdellah, BP 1893, Km 22, Road of Sidi Harazem, Fez, MoroccoLaboratory of Biotechnology, Conservation, and Valorisation of Natural Resources, Faculty of Sciences Dhar ElMahraz, Sidi Mohammed Ben Abdallah University, P.O. Box 1796 (Atlas), Fez30000, MoroccoDepartment of Food Science, Faculty of Agricultural and Food Sciences, Laval University, 2325 Quebec City, QC G1V 0A6, CanadaDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, 11 P.O. Box 2460, Riyadh11451, Saudi ArabiaDepartment of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune70000, MoroccoDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh11433, Saudi ArabiaLaboratory of Biotechnology and Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), 30000Fez, MoroccoThe objective of this work was to characterize the phytochemical composition of essential oil from Juniperus thurifera (L.) fruits (EOFT) and study its antioxidant, antibacterial, and antifungal effects. EOFT was extracted by hydrodistillation and fingerprinted by using GC–MS. The antioxidant effect of EOFT was evaluated using 2,2-diphenylpicrylhydrazyl (DPPH), ferric iron reduction assay (FRAP), and total antioxidant capacity (TAC) assays. Importantly, the antimicrobial activity of EOFT was performed against Candida albicans, Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Proteus mirabilis. In addition, the inhibitory capacity of NADPH oxidase and human acetylcholinesterase was also investigated using molecular docking. The results of the chemical composition reveal that EOFT constituted 11 terpenic compounds with dominance of elemol (33.86%), terpinen-4-ol (27.80%), and cryptomeridiol (18.36%). The antioxidant power of EOFT recorded IC50 values of 197.07 ± 0.09 μg/mL (DPPH) and 216.34 ± 0.06 μg/mL (FRAP), while TAC of EOFT was determined to be 181.06 μg AAE/mg. The antibacterial potency on solid medium revealed that EOFT induced inhibition zone diameters reaching 14 mm, and a minimum concentration up to 2.78 µg/mL against the studied bacterial strains. The EOFT also showed an important antifungal effect as the inhibition reached 42%, and the MIC was between 7.50 and 22.25 µg/mL. The in silico study showed that o-Cymene was the most active molecule against NAD(P)H oxidase followed by cadinol with a Glide score of −5.344 and −5.143 kcal/mol, respectively. Due to their promising results, the outcome of this work suggests that EOFT could be used as an interesting natural weapon to control microbial and freed radical-related diseases.https://doi.org/10.1515/chem-2022-0332spanish juniperterpene compoundsgc/msmedicinal plantantifungalantibacterial |
| spellingShingle | Lafraxo Soufyane Zouirech Otmane El Barnossi Azeddin Chelouati Tarik Chebaibi Mohamed Chebbac Khalid Nafidi Hiba-Allah Salamatullah Ahmad Mohammad Bourhia Mohammed Aboul-Soud Mourad A. M. Bari Amina Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations Open Chemistry spanish juniper terpene compounds gc/ms medicinal plant antifungal antibacterial |
| title | Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations |
| title_full | Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations |
| title_fullStr | Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations |
| title_full_unstemmed | Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations |
| title_short | Promising antioxidant and antimicrobial effects of essential oils extracted from fruits of Juniperus thurifera: In vitro and in silico investigations |
| title_sort | promising antioxidant and antimicrobial effects of essential oils extracted from fruits of juniperus thurifera in vitro and in silico investigations |
| topic | spanish juniper terpene compounds gc/ms medicinal plant antifungal antibacterial |
| url | https://doi.org/10.1515/chem-2022-0332 |
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