Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing
Diazotrophic communities make an essential contribution to the productivity through providing new nitrogen. However, knowledge of the roles that both mangrove tree species and geochemical parameters play in shaping mangove rhizosphere diazotrophic communities is still elusive. Here, a comprehensive...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02032/full |
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| author | Yanying Zhang Yanying Zhang Yanying Zhang Qingsong Yang Qingsong Yang Juan Ling Joy D. Van Nostrand Zhou Shi Jizhong Zhou Junde Dong Junde Dong |
| author_facet | Yanying Zhang Yanying Zhang Yanying Zhang Qingsong Yang Qingsong Yang Juan Ling Joy D. Van Nostrand Zhou Shi Jizhong Zhou Junde Dong Junde Dong |
| author_sort | Yanying Zhang |
| collection | DOAJ |
| description | Diazotrophic communities make an essential contribution to the productivity through providing new nitrogen. However, knowledge of the roles that both mangrove tree species and geochemical parameters play in shaping mangove rhizosphere diazotrophic communities is still elusive. Here, a comprehensive examination of the diversity and structure of microbial communities in the rhizospheres of three mangrove species, Rhizophora apiculata, Avicennia marina, and Ceriops tagal, was undertaken using high-throughput sequencing of the 16S rRNA and nifH genes. Our results revealed a great diversity of both the total microbial composition and the diazotrophic composition specifically in the mangrove rhizosphere. Deltaproteobacteria and Gammaproteobacteria were both ubiquitous and dominant, comprising an average of 45.87 and 86.66% of total microbial and diazotrophic communities, respectively. Sulfate-reducing bacteria belonging to the Desulfobacteraceae and Desulfovibrionaceae were the dominant diazotrophs. Community statistical analyses suggested that both mangrove tree species and additional environmental variables played important roles in shaping total microbial and potential diazotroph communities in mangrove rhizospheres. In contrast to the total microbial community investigated by analysis of 16S rRNA gene sequences, most of the dominant diazotrophic groups identified by nifH gene sequences were significantly different among mangrove species. The dominant diazotrophs of the family Desulfobacteraceae were positively correlated with total phosphorus, but negatively correlated with the nitrogen to phosphorus ratio. The Pseudomonadaceae were positively correlated with the concentration of available potassium, suggesting that diazotrophs potentially play an important role in biogeochemical cycles, such as those of nitrogen, phosphorus, sulfur, and potassium, in the mangrove ecosystem. |
| first_indexed | 2024-12-11T08:56:01Z |
| format | Article |
| id | doaj.art-608b52871d0b48a6a0f49c870ce52885 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T08:56:01Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-608b52871d0b48a6a0f49c870ce528852022-12-22T01:13:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-10-01810.3389/fmicb.2017.02032269735Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput SequencingYanying Zhang0Yanying Zhang1Yanying Zhang2Qingsong Yang3Qingsong Yang4Juan Ling5Joy D. Van Nostrand6Zhou Shi7Jizhong Zhou8Junde Dong9Junde Dong10CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaTropical Marine Biological Research Station in Hainan, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Sanya, ChinaDepartment of Microbiology and Plant Biology, Institute for Environmental Genomics, University of Oklahoma, Norman, OK, United StatesCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaDepartment of Microbiology and Plant Biology, Institute for Environmental Genomics, University of Oklahoma, Norman, OK, United StatesDepartment of Microbiology and Plant Biology, Institute for Environmental Genomics, University of Oklahoma, Norman, OK, United StatesDepartment of Microbiology and Plant Biology, Institute for Environmental Genomics, University of Oklahoma, Norman, OK, United StatesCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaTropical Marine Biological Research Station in Hainan, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Sanya, ChinaDiazotrophic communities make an essential contribution to the productivity through providing new nitrogen. However, knowledge of the roles that both mangrove tree species and geochemical parameters play in shaping mangove rhizosphere diazotrophic communities is still elusive. Here, a comprehensive examination of the diversity and structure of microbial communities in the rhizospheres of three mangrove species, Rhizophora apiculata, Avicennia marina, and Ceriops tagal, was undertaken using high-throughput sequencing of the 16S rRNA and nifH genes. Our results revealed a great diversity of both the total microbial composition and the diazotrophic composition specifically in the mangrove rhizosphere. Deltaproteobacteria and Gammaproteobacteria were both ubiquitous and dominant, comprising an average of 45.87 and 86.66% of total microbial and diazotrophic communities, respectively. Sulfate-reducing bacteria belonging to the Desulfobacteraceae and Desulfovibrionaceae were the dominant diazotrophs. Community statistical analyses suggested that both mangrove tree species and additional environmental variables played important roles in shaping total microbial and potential diazotroph communities in mangrove rhizospheres. In contrast to the total microbial community investigated by analysis of 16S rRNA gene sequences, most of the dominant diazotrophic groups identified by nifH gene sequences were significantly different among mangrove species. The dominant diazotrophs of the family Desulfobacteraceae were positively correlated with total phosphorus, but negatively correlated with the nitrogen to phosphorus ratio. The Pseudomonadaceae were positively correlated with the concentration of available potassium, suggesting that diazotrophs potentially play an important role in biogeochemical cycles, such as those of nitrogen, phosphorus, sulfur, and potassium, in the mangrove ecosystem.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02032/fullmangrovesmicrobial communitydiazotrophsnifHhigh-throughput sequencingsulfate-reducing bacteria |
| spellingShingle | Yanying Zhang Yanying Zhang Yanying Zhang Qingsong Yang Qingsong Yang Juan Ling Joy D. Van Nostrand Zhou Shi Jizhong Zhou Junde Dong Junde Dong Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing Frontiers in Microbiology mangroves microbial community diazotrophs nifH high-throughput sequencing sulfate-reducing bacteria |
| title | Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing |
| title_full | Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing |
| title_fullStr | Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing |
| title_full_unstemmed | Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing |
| title_short | Diversity and Structure of Diazotrophic Communities in Mangrove Rhizosphere, Revealed by High-Throughput Sequencing |
| title_sort | diversity and structure of diazotrophic communities in mangrove rhizosphere revealed by high throughput sequencing |
| topic | mangroves microbial community diazotrophs nifH high-throughput sequencing sulfate-reducing bacteria |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02032/full |
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