Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations)
Background and purpose: Adipose - drived stem cells are multipotent cells that capable to diffrentiate in to osteogenic cells. On the other hand, the scaffold of chitosan and gelatin are biodegradable and compatible used for tissue repair. This study evaluated the repair of femoral bone defect usin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2015-08-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1989-1&slc_lang=en&sid=1 |
| _version_ | 1818066429592731648 |
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| author | S Salehy shemiran M Sadooghi H Sadraie GR Kaka |
| author_facet | S Salehy shemiran M Sadooghi H Sadraie GR Kaka |
| author_sort | S Salehy shemiran |
| collection | DOAJ |
| description | Background and purpose: Adipose - drived stem cells are multipotent cells that capable to diffrentiate in to osteogenic cells. On the other hand, the scaffold of chitosan and gelatin are biodegradable and compatible used for tissue repair. This study evaluated the repair of femoral bone defect using adipose-derived stem cells on gelatin – chitosan membrane in adult Albino Wistar rats by radiographic and biomechanical tests.
Materials and Methods: In this study, sixty male adult rats were equally divided into six groups. 1)Normal group) that received no treatment and bone intact and is only used to compare the biomechanics, 2(Normal Foramen group) femoral bone were removed from the animal body, and were then created bone defect similar to the experimental groups for biomechanic testing, 3 (control group) received no treatment after bone defect. 4 (sham group) after bone defect, the culture medium was injected at the site of bone defect. 5 (gelatin - chitosan group) that gelatin - chitosan membrane was used in to bone defect. Group 6 that transplanted ADSCs in to bone defect.
Results: Radiograph bone opacity increased in chitosan – gelatin, cell and cell with chitosan - gelatin groups compared to control group but this difference was not significant. Biomechanical results of bone showed mean Fmax was increased in treatment groups compared to control group however the difference was not significant but compared with the normal foramen group significantly increased.
Conclusion: It seems that scaffold of chitosan - gelatin membrane and ADSC cell transplantation appears to be effective in repair of bone defect. |
| first_indexed | 2024-12-10T15:07:39Z |
| format | Article |
| id | doaj.art-608c2bae9a1c4f639c1586773b16e655 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-10T15:07:39Z |
| publishDate | 2015-08-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-608c2bae9a1c4f639c1586773b16e6552022-12-22T01:44:01ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332015-08-01235420431Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations)S Salehy shemiran0M Sadooghi1H Sadraie2GR Kaka3 Islamic Azad University of Tehran Islamic Azad University of Tehran Baqiyatallah University of Medical Sciences Baqiyatallah University of Medical Sciences Background and purpose: Adipose - drived stem cells are multipotent cells that capable to diffrentiate in to osteogenic cells. On the other hand, the scaffold of chitosan and gelatin are biodegradable and compatible used for tissue repair. This study evaluated the repair of femoral bone defect using adipose-derived stem cells on gelatin – chitosan membrane in adult Albino Wistar rats by radiographic and biomechanical tests. Materials and Methods: In this study, sixty male adult rats were equally divided into six groups. 1)Normal group) that received no treatment and bone intact and is only used to compare the biomechanics, 2(Normal Foramen group) femoral bone were removed from the animal body, and were then created bone defect similar to the experimental groups for biomechanic testing, 3 (control group) received no treatment after bone defect. 4 (sham group) after bone defect, the culture medium was injected at the site of bone defect. 5 (gelatin - chitosan group) that gelatin - chitosan membrane was used in to bone defect. Group 6 that transplanted ADSCs in to bone defect. Results: Radiograph bone opacity increased in chitosan – gelatin, cell and cell with chitosan - gelatin groups compared to control group but this difference was not significant. Biomechanical results of bone showed mean Fmax was increased in treatment groups compared to control group however the difference was not significant but compared with the normal foramen group significantly increased. Conclusion: It seems that scaffold of chitosan - gelatin membrane and ADSC cell transplantation appears to be effective in repair of bone defect.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1989-1&slc_lang=en&sid=1: ADSCs Chitosan–Gelatin membrane Cell transplantation Bone Repair Rat |
| spellingShingle | S Salehy shemiran M Sadooghi H Sadraie GR Kaka Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd : ADSCs Chitosan–Gelatin membrane Cell transplantation Bone Repair Rat |
| title | Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) |
| title_full | Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) |
| title_fullStr | Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) |
| title_full_unstemmed | Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) |
| title_short | Study of the repair of femoral bone defects using adipose-derived stem cells (ADSCs) on Chitosan - Gelatin membrane in rats (biomechanical and radiological evaluations) |
| title_sort | study of the repair of femoral bone defects using adipose derived stem cells adscs on chitosan gelatin membrane in rats biomechanical and radiological evaluations |
| topic | : ADSCs Chitosan–Gelatin membrane Cell transplantation Bone Repair Rat |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1989-1&slc_lang=en&sid=1 |
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