Optic nerve head astrocytes contribute to vascular associated effects
PurposeThis study was conducted in order to test the expression of vasoactive substances within rat lamina cribrosa (LC) and optic nerve head (ONH) astrocytes, so as to investigate the role and potential mechanism of ONH astrocytes in vascular associated effects.MethodsLC tissue sections and primary...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.943986/full |
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| author | Yanmin Dong Yanmin Dong Yue Fu Xiaobing Qian Leilei Lin Yongguang Yuan Yujie Li Wanwen Shao Qianying Gao |
| author_facet | Yanmin Dong Yanmin Dong Yue Fu Xiaobing Qian Leilei Lin Yongguang Yuan Yujie Li Wanwen Shao Qianying Gao |
| author_sort | Yanmin Dong |
| collection | DOAJ |
| description | PurposeThis study was conducted in order to test the expression of vasoactive substances within rat lamina cribrosa (LC) and optic nerve head (ONH) astrocytes, so as to investigate the role and potential mechanism of ONH astrocytes in vascular associated effects.MethodsLC tissue sections and primary cultured ONH astrocytes were obtained from adult Sprague-Dawley (SD) rats. Immunofluorescent staining was then used to detect the expression of vasoactive substances. Hyperoxia exposure was carried out both in vivo and in vitro, after which nitric oxide (NO) levels in LC tissue and cell supernatant were detected. The variations of protein and gene expression associated with vasoactive substances were subsequently tested. ONH astrocytes and vascular smooth muscle cells (VSMCs) were then incubated in a direct co-culture manner. Morphological parameters of VSMCs were finally analyzed in order to evaluate cell contraction.ResultsEndothelin-1 (ET-1), nitric oxide synthase (NOS) and renin-angiotensin system (RAS) were detected in both LC tissue and ONH astrocytes. Retinal vessel diameter was found obviously decreased following hyperoxia exposure. Moreover, hyperoxia inhibited NO production both in vivo and in vitro. ET-1 and RAS elements were observed to be upregulated, whereas NOS was downregulated. In ONH astrocytes and VSMCs co-culture system, the length-to-width ratio of VSMCs was shown to significantly increase on days 3 and 7 in hyperoxia compared with normoxia.ConclusionsThere is an abundance of expression of vasoactive substances within LC tissue and ONH astrocytes. The contractile response of VSMCs in the co-culture system provided direct evidence for the involvement of ONH astrocytes in vascular associated effects, which may signify a potentially novel direction for future research. |
| first_indexed | 2024-12-10T09:01:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-10T09:01:59Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-608c74a1627b471b87bad94c784f79f42022-12-22T01:55:15ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-07-01910.3389/fmed.2022.943986943986Optic nerve head astrocytes contribute to vascular associated effectsYanmin Dong0Yanmin Dong1Yue Fu2Xiaobing Qian3Leilei Lin4Yongguang Yuan5Yujie Li6Wanwen Shao7Qianying Gao8Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, ChinaPurposeThis study was conducted in order to test the expression of vasoactive substances within rat lamina cribrosa (LC) and optic nerve head (ONH) astrocytes, so as to investigate the role and potential mechanism of ONH astrocytes in vascular associated effects.MethodsLC tissue sections and primary cultured ONH astrocytes were obtained from adult Sprague-Dawley (SD) rats. Immunofluorescent staining was then used to detect the expression of vasoactive substances. Hyperoxia exposure was carried out both in vivo and in vitro, after which nitric oxide (NO) levels in LC tissue and cell supernatant were detected. The variations of protein and gene expression associated with vasoactive substances were subsequently tested. ONH astrocytes and vascular smooth muscle cells (VSMCs) were then incubated in a direct co-culture manner. Morphological parameters of VSMCs were finally analyzed in order to evaluate cell contraction.ResultsEndothelin-1 (ET-1), nitric oxide synthase (NOS) and renin-angiotensin system (RAS) were detected in both LC tissue and ONH astrocytes. Retinal vessel diameter was found obviously decreased following hyperoxia exposure. Moreover, hyperoxia inhibited NO production both in vivo and in vitro. ET-1 and RAS elements were observed to be upregulated, whereas NOS was downregulated. In ONH astrocytes and VSMCs co-culture system, the length-to-width ratio of VSMCs was shown to significantly increase on days 3 and 7 in hyperoxia compared with normoxia.ConclusionsThere is an abundance of expression of vasoactive substances within LC tissue and ONH astrocytes. The contractile response of VSMCs in the co-culture system provided direct evidence for the involvement of ONH astrocytes in vascular associated effects, which may signify a potentially novel direction for future research.https://www.frontiersin.org/articles/10.3389/fmed.2022.943986/fullastrocytesoptic nerve headvascular regulationendothelin-1nitric oxide synthaserenin-angiotensin system |
| spellingShingle | Yanmin Dong Yanmin Dong Yue Fu Xiaobing Qian Leilei Lin Yongguang Yuan Yujie Li Wanwen Shao Qianying Gao Optic nerve head astrocytes contribute to vascular associated effects Frontiers in Medicine astrocytes optic nerve head vascular regulation endothelin-1 nitric oxide synthase renin-angiotensin system |
| title | Optic nerve head astrocytes contribute to vascular associated effects |
| title_full | Optic nerve head astrocytes contribute to vascular associated effects |
| title_fullStr | Optic nerve head astrocytes contribute to vascular associated effects |
| title_full_unstemmed | Optic nerve head astrocytes contribute to vascular associated effects |
| title_short | Optic nerve head astrocytes contribute to vascular associated effects |
| title_sort | optic nerve head astrocytes contribute to vascular associated effects |
| topic | astrocytes optic nerve head vascular regulation endothelin-1 nitric oxide synthase renin-angiotensin system |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.943986/full |
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